Detection and identification of vegetative insecticidal proteins vip3 genes of Bacillus thuringiensis strains using polymerase chain reaction-high resolution melt analysis.

abstract：:It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the p...

journal_title：Current microbiology

authors： Song D,Shi B,Xue H,Li Y,Yu B,Xu Z,Liu F,Li J

更新日期：2006-01-01 00:00:00

abstract：:By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...

journal_title：Current microbiology

authors： Dai Y,Liu J,Zheng C,Wu A,Zeng J,Qiu G

更新日期：2009-11-01 00:00:00

abstract：:Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main fl...

journal_title：Current microbiology

authors： Castañón-Rodríguez JF,Pérez-Rodríguez N,de Souza Oliveira RP,Aguilar-Uscanga MG,Domínguez JM

更新日期：2016-10-01 00:00:00

abstract：:Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli....

journal_title：Current microbiology

authors： Specht KM,Sheetz KH,Alexander CM,Lamech LT,O'Connor LH,Walker DM,Stevenson HP

更新日期：2010-04-01 00:00:00

abstract：:Butterfly stain, common in Chilean tepa (Laureliopsis philippiana [Looser] Schodde) trees, appears in cross-section of the stem as a series of partially overlapping orange-brown arcs that in early stages resembles a butterfly. Bacterial isolates from stained tepa wood samples cultured under aerobic and anaerobic condi...

journal_title：Current microbiology

authors： McLemore JB,Tainter FH,McElreath SD

更新日期：2000-02-01 00:00:00

abstract：:Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

journal_title：Current microbiology

authors： Iwaki H,Nakai E,Nakamura S,Hasegawa Y

更新日期：2008-08-01 00:00:00

abstract：:To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...

journal_title：Current microbiology

authors： Kim HS,Lee DW,Woo SD,Yu YM,Kang SK

更新日期：1998-07-01 00:00:00

abstract：:A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldo...

journal_title：Current microbiology

authors： Palacios GC,Timmons BC,Eskew EK,Solórzano F,Mattingly SJ

更新日期：2003-10-01 00:00:00

abstract：:The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A to...

journal_title：Current microbiology

authors： Al-Talib H,Yean CY,Al-khateeb A,Singh KK,Hasan H,Al-Jashamy K,Ravichandran M

更新日期：2010-07-01 00:00:00

abstract：:Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding s...

journal_title：Current microbiology

authors： Kiser KB,Schmidt MG

更新日期：1999-02-01 00:00:00

abstract：:The evolution of the symbiotic association with microbes allowed termites to decompose ingested lignocellulose from plant-derived substrates, including herbivore dung and soil humus. Representatives of the Syntermitinae (Termitidae) range in their feeding habits from wood and litter-feeding to humus-feeding species. H...

journal_title：Current microbiology

authors： Moreira EA,Alvarez TM,Persinoti GF,Paixão DAA,Menezes LR,Cairo JPF,Squina FM,Costa-Leonardo AM,Carrijo T,Arab A

更新日期：2018-12-01 00:00:00

abstract：:Adenosine 5'-phosphosulfate (APS) reductase is a key enzyme involved in the pathways of sulfate reduction and sulfide oxidation in the biological sulfur cycle. In this study, the gene of APS reductase from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the soluble protein was purified by ...

journal_title：Current microbiology

authors： Zheng C,Zhang Y,Liu Y,Wu A,Xia L,Zeng J,Liu J,Qiu G

更新日期：2009-06-01 00:00:00

abstract：:Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...

journal_title：Current microbiology

authors： Yu X,Liu T,Sun Z,Guan P,Zhu J,Wang S,Li S,Deng Q,Wang L,Zheng A,Li P

更新日期：2012-04-01 00:00:00

abstract：:Biofouling leads to water quality deterioration and higher maintenance cost for cleaning of membranes. The present study has demonstrated the application of a biomolecule (vanillin) in scrubbing and destabilizing biofilms of drinking water reverse osmosis (RO) membrane module in lab scale reactor set-up. Reverse osmos...

journal_title：Current microbiology

authors： Pal S,Qureshi A,Purohit HJ

更新日期：2020-09-01 00:00:00

abstract：:The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...

journal_title：Current microbiology

authors： Prasad S,Pratibha MS,Manasa P,Buddhi S,Begum Z,Shivaji S

更新日期：2013-01-01 00:00:00

abstract：:This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...

journal_title：Current microbiology

authors： Shi Y,Wu S,Li W,Liu D,Ma G,Zhang Z,Yuan L,Li H,Zhang S,Mushtaq N,Xu J

更新日期：2020-12-01 00:00:00

abstract：:(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control ...

journal_title：Current microbiology

authors： Appanna VD,Hamel RD,Lévasseur R

更新日期：2003-07-01 00:00:00

abstract：:In the last few years, numerous cases of multidrug-resistant Achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. To gain insights into the molecular mechanisms and mobile elements related to multidrug resistance in this bacterium, we studied 24 non-epidemiologi...

journal_title：Current microbiology

authors： Traglia GM,Almuzara M,Merkier AK,Adams C,Galanternik L,Vay C,Centrón D,Ramírez MS

更新日期：2012-12-01 00:00:00

abstract：:The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...

journal_title：Current microbiology

authors： Strohl WR,Larkin JM

更新日期：1978-01-01 00:00:00

abstract：:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...

journal_title：Current microbiology

authors： Puri S,Beg QK,Gupta R

更新日期：2002-04-01 00:00:00

abstract：:Soil microcosm experiments were used to investigate the effects on growth and activity of soil microorganisms of an herbicide combination (60% bromoxynil + 3% prosulfuron) frequently used to provide a broad spectrum control of weed species. Culturable aerobic bacteria, fungi, and actinomycetes, the fundamental groups ...

journal_title：Current microbiology

authors： Pampulha ME,Oliveira A

更新日期：2006-09-01 00:00:00

abstract：:Biofilm-forming bacteria Pseudomonas aeruginosa is a common pathogen in mechanically ventilated newborns, which can cause life-threatening infections. Alginate of mucoid Pseudomonas aeruginosa biofilms is considered an important virulence factor which contributes to the resistance to antibiotics. Traditionally, ambrox...

journal_title：Current microbiology

authors： Li F,Yu J,Yang H,Wan Z,Bai D

更新日期：2008-07-01 00:00:00

abstract：:Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce t...

journal_title：Current microbiology

authors： Dawson HN,Burlingame R,Cannons AC

更新日期：1997-12-01 00:00:00

abstract：:Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encodin...

journal_title：Current microbiology

authors： Chen YC,Chang MC,Chuang YC,Jeang CL

更新日期：2004-09-01 00:00:00

abstract：:Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...

journal_title：Current microbiology

authors： Gu Q,Yang ZH,Zhao DM,Zhang D,Wang Q,Ma LS,Zhu JH

更新日期：2017-09-01 00:00:00

abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...

journal_title：Current microbiology

authors： Bhatti M,MacRobert A,Henderson B,Wilson M

更新日期：2002-08-01 00:00:00

abstract：:The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. The gene of ferred...

journal_title：Current microbiology

authors： Zeng J,Huang X,Liu Y,Liu J,Qiu G

更新日期：2007-12-01 00:00:00

abstract：:Production of carbon dioxide, as one of the ultimate products of fungal metabolism, can be used to quantify and measure their metabolic rate under different conditions, thus aiding in finding the optimal substrate and environment for cultivation of wood-destroying fungi. This study is focused on species Pleurotus ostr...

journal_title：Current microbiology

authors： Pavlík M,Fleischer P,Fleischer P Jr,Pavlík M Jr,Šuleková M

更新日期：2020-09-01 00:00:00

abstract：:Ansamitocins are extraordinarily potent antitumor agents. Ansamitocin P-3 (AP-3), which is produced by Actinosynnema pretiosum, has been developed as a cytotoxic drug for breast cancer. Despite its importance, AP-3 is of limited applicability because of the low production yield. A. pretiosum strain X47 was developed f...

journal_title：Current microbiology

authors： Zhong C,Zong G,Qian S,Liu M,Fu J,Zhang P,Li J,Cao G

更新日期：2019-08-01 00:00:00

abstract：:The nucleotide sequences of 16S rDNAs (coding for the small subunit ribosomal RNAs) were used to identify Xylella fastidiosa, a nutritionally fastidious plant pathogenic bacterium. The near-complete 16S rDNAs from nine strains of Xyl. fastidiosa, including seven pathotypes and one strain of Xanthomonas campestris pv. ...

journal_title：Current microbiology

authors： Chen J,Banks D,Jarret RL,Chang CJ,Smith BJ

更新日期：2000-01-01 00:00:00