Abstract：
:A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldolase was identified as responsible for this characteristic. In the present study, the HVC was sought in a collection of 57 GBS isolates by hybridization with a probe containing a putative aldolase gene on genomic DNA restriction enzyme digests. Isolates were initially classified as HVC or non-HVC by their inability to grow at 40 degrees C. Three serotype III invasive isolates had the HVC control restriction/hybridization pattern. They were also unable to grow at 40 degrees C. The remaining 11 invasive and all carrier isolates showed a pattern identical to that of the non-HVC control. These results provide additional support for the existence of a highly virulent clonal group among serotype III isolates and suggest that hybridization with a probe containing the aldolase gene on DNA restriction enzyme digests can be an alternative method for identifying highly virulent isolates.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Palacios GC,Timmons BC,Eskew EK,Solórzano F,Mattingly SJdoi
10.1007/s00284-002-3957-5keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
319-22issue
4eissn
0343-8651issn
1432-0991journal_volume
47pub_type
杂志文章abstract：:A new antagonistic Burkholderia strain, designated MP-1 and producing antifungal activities against various filamentous plant pathogenic fungi, was isolated from the rhizoshere in the Naju area. Cultural characteristic studies strongly suggested that this strain belongs to the genus Burkholderia. The nucleotide sequen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0333-2
更新日期：2006-11-01 00:00:00
abstract：:Lectins LI and LII, localized on the surface of the nitrogen-fixing soil bacterium Bacillus polymyxa 1460, were shown to possess proteolytic activity. A relationship was found between the proteolytic and hemagglutinating activities of the lectins. Blocking of hemagglutinating activity with specific carbohydrate hapten...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010128
更新日期：2000-10-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9608-3
更新日期：2010-10-01 00:00:00
abstract：:Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological control activities, promote plant health or cause plant disease. Here, we present the draft genome sequence of the kiwifrui...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01655-2
更新日期：2019-05-01 00:00:00
abstract：:Pseudomonas aeruginosa is a leading cause of hospital-acquired infections and difficult to treat due to acquired-resistance to multiple antibiotics. A pair of strains, M38100A and M38100B, previously identified from a single clinical isolate of P. aeruginosa was investigated to understand phenotypic and genotypic char...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9570-0
更新日期：2010-07-01 00:00:00
abstract：:Potassium ion pool was studied in glycolyzing Enterococcus hirae, grown at high or low alkaline pH (pH 9.5 and 8.0, respectively). Energy-dependent increase of K+ pool was lower for the wild-type cells, grown at pH 9.5, than that for the cells grown at pH 8.0. It was inhibited by N,N'-dicyclohexylcarbodiimide (DCCD). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900289
更新日期：1998-02-01 00:00:00
abstract：:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0085-2
更新日期：2007-09-01 00:00:00
abstract：:A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71T, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high sim...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01778-6
更新日期：2019-12-01 00:00:00
abstract：:A Gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated S7-3-3T, was isolated from apple orchard soil in Gyeongsangnam-do province, South Korea, and was characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S7-3-3T belong...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1434-z
更新日期：2018-06-01 00:00:00
abstract：:Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studie...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3740-7
更新日期：2002-10-01 00:00:00
abstract：:The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4358-8
更新日期：2004-10-01 00:00:00
abstract：:This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4491-z
更新日期：2005-07-01 00:00:00
abstract：:We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4103-8
更新日期：2003-10-01 00:00:00
abstract：:Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, patho...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01647-2
更新日期：2019-04-01 00:00:00
abstract：:Low-substrate regulated microaerophilic behavior (LSRMB) was observed in 10-54% of the bacteria isolated from several fresh-water lakes or ponds, subsurface soils, activated sludge, and Antarctic dry valley soils. Five Pseudomonas and two Bacillus type species showed LSRMB. A subsurface Pseudomonas jessenii strain was...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010097
更新日期：2000-08-01 00:00:00
abstract：:In the present study, EMA (ethidium monoazide) treatment was applied to a silty-sand reference soil prior to DNA extraction to enable a differentiation between dead and living cells. For this purpose, a reference soil was spiked with Listeria monocytogenes cells or cell equivalents, respectively. With the purpose of e...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01772-y
更新日期：2019-12-01 00:00:00
abstract：:Moutai Jiuqu is a famous aromatic raw material of Maotai flavor liquor in China. It is brewed at high temperature and contains many kinds of bacteria, molds, and yeasts. There are many useful glycoside hydrolases in these microfloras, from which efficient glycoside hydrolases can be screened for biotransformation of n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02078-0
更新日期：2020-10-01 00:00:00
abstract：:Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1284-0
更新日期：2017-09-01 00:00:00
abstract：:A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0584-x
更新日期：2014-09-01 00:00:00
abstract：:We constructed recombinant phage particles displaying the Bacillus thuringiensis Cry1Ba4 active toxin using the pfUSE5 and pComb3X phagemid vectors. The recombinant phage particles were screened and evaluated for displayed biologically active Cry1Ba4 toxin against the target insect larvae. Concurrent expression of Cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0164-9
更新日期：2006-11-01 00:00:00
abstract：:The interactions between the proteolytic X2L strain of Oenococcus oeni and the non-proteolytic 12p strain of Pediococcus pentosaceus were assayed. The characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. O. oeni showed poor cell grow...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0079-x
更新日期：2006-02-01 00:00:00
abstract：:In this article, we investigated the effect of exogenous calcium on improving viability of Debaryomyces hansenii and Pichia membranaefaciens under heat stress, and evaluated the role of calcium in reducing oxidant damage of proteins in the yeast cells. The results indicated that high concentration of exogenous calcium...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0133-4
更新日期：2012-08-01 00:00:00
abstract：:Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900438
更新日期：1999-09-01 00:00:00
abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0910-y
更新日期：2016-01-01 00:00:00
abstract：:A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1553-6
更新日期：2018-11-01 00:00:00
abstract：:A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella and Spodoptera exigua was isolated from a Korean soil sample and characterized. The isolate, named B. thuringiensis K1, was determined to belong to ssp. kurstaki (H3a3b3c) type by an H antisera agglutination test and prod...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3755-0
更新日期：2002-10-01 00:00:00
abstract：:Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9347-x
更新日期：2009-04-01 00:00:00
abstract：:In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0192-x
更新日期：2006-06-01 00:00:00
abstract：:Biofouling leads to water quality deterioration and higher maintenance cost for cleaning of membranes. The present study has demonstrated the application of a biomolecule (vanillin) in scrubbing and destabilizing biofilms of drinking water reverse osmosis (RO) membrane module in lab scale reactor set-up. Reverse osmos...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02003-5
更新日期：2020-09-01 00:00:00
abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
