Abstract：
:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crystals of the same bipyramidal shape; however, they revealed a variety of number and type of cry genes. Genes cry1I, cry2Ab, and cry9B were the most frequently observed in epizootic strains. Gene cry1I was noted in of 50% epizootic isolates. Eighty-three percent of them harbored gene cry2Ab. Gene cry9B was found for 42% of strains isolated during epizootics. Three isolates showed the largest number of cry genes and their variety; hence, they were chosen for the toxicity assay of their crystals and spores on C. pomonella larvae. One of them had approximately sixfold higher insecticidal activity than the reference strain B. thuringiensis subsp. kurstaki BTK STANDARD.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Konecka E,Kaznowski A,Ziemnicka J,Ziemnicki K,Paetz Hdoi
10.1007/s00284-007-0085-2subject
Has Abstractpub_date
2007-09-01 00:00:00pages
217-22issue
3eissn
0343-8651issn
1432-0991journal_volume
55pub_type
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