Abstract：
:The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expression in Escherichia coli revealed that each of the genes encodes fructose-1,6-bisphosphatase (FBPase). A closely related activity likewise operating in the Calvin carbon reduction cycle, sedoheptulose-1,7-bisphosphatase, was also catalyzed by the two isoenzymes which were purified from autotrophically grown cells of A. eutrophus. Two-dimensional gel electrophoresis allowed the separation of the cbbF gene products. Preliminary physical evidence by Southern hybridization with a heterologous gene probe was obtained for the existence of a third FBPase gene, fbp, on the chromosome of the organism. Its product is probably involved in the heterotrophic carbon metabolism.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Yoo JG,Bowien Bdoi
10.1007/BF00294635subject
Has Abstractpub_date
1995-07-01 00:00:00pages
55-61issue
1eissn
0343-8651issn
1432-0991journal_volume
31pub_type
杂志文章abstract：:Bacterial communities communicate, regulate and coordinate their cooperative activities and physiological process by releasing, sensing and responding to small diffusible signal molecules such as acyl homoserine lactones (AHLs), auto-inducing oligo-peptides (AIPs) and autoinducer-2, a process referred to as Quorum sen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02183-0
更新日期：2020-11-01 00:00:00
abstract：:The ability of non-tuberculous mycobacteria to form biofilms may allow for their increased resistance to currently used biocides in medical and industrial settings. This study examines the biofilm growth of Mycobacterium fortuitum and Mycobacterium marinum, using the MBEC trade mark assay system, and compares the susc...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3796-4
更新日期：2003-01-01 00:00:00
abstract：:Bipolaris oryzae is a filamentous ascomycetous fungus that causes brown leaf spot disease in rice. We isolated and characterized BLR2, a gene that encodes a putative blue-light regulator similar to Neurospora crassa white collar-2 (WC-2). The deduced amino acid sequence of the BLR2 showed significant homology to other...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9080-x
更新日期：2008-02-01 00:00:00
abstract：:A new cry gene (cry1Ca9) was cloned and sequenced from a Bacillus thuringiensis isolate native to Taiwan (G10-01A). The cry1C-type gene, designated cry1Ca9, consisted of an open reading frame of 3,567 bp, encoding a protein of 1,189 amino acid residues. The polypeptide has the deduced amino acid sequences predicting m...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3982-4
更新日期：2003-10-01 00:00:00
abstract：:Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1262-6
更新日期：2017-08-01 00:00:00
abstract：:A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900035
更新日期：1996-04-01 00:00:00
abstract：:Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0483-6
更新日期：2014-03-01 00:00:00
abstract：:This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was exa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9438-3
更新日期：2009-09-01 00:00:00
abstract：:A Gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated S7-3-3T, was isolated from apple orchard soil in Gyeongsangnam-do province, South Korea, and was characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S7-3-3T belong...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1434-z
更新日期：2018-06-01 00:00:00
abstract：:A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71T, was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high sim...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01778-6
更新日期：2019-12-01 00:00:00
abstract：:Clostridium (Clostridioides) difficile ribotype 027 (RT027) was detected in Germany for the first time in 2007 during an outbreak in the region of Trier, Rhineland-Palatinate and is today the most prevalent ribotype (RT) in Europe. We aimed to determine the changes in RT distribution and corresponding antimicrobial re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01654-3
更新日期：2019-04-01 00:00:00
abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
abstract：:The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9107-y
更新日期：2008-05-01 00:00:00
abstract：:Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and ex...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0114-5
更新日期：2002-09-01 00:00:00
abstract：:The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006784
更新日期：1999-03-01 00:00:00
abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0977-5
更新日期：2016-04-01 00:00:00
abstract：:Stenotrophomonas maltophilia is an emerging opportunistic pathogen linked not only to bacteremia, sepsis, and pneumonia but also to severe chronic enteritis. Persons with the impaired immune system are prone to be infected by S. maltophilia since its pathogenicity seems to be more associated with the host immune syste...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1559-0
更新日期：2018-12-01 00:00:00
abstract：:An isolate of Lactococcus lactis from fermented milk was found to produce a bacteriocin peptide. The isolate could grow in a medium with an initial pH of 11.0, in which it produced the bacteriocin extracellularly at the highest level. The level of the bacteriocin in the medium increased in parallel to the bacterial gr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4545-2
更新日期：2005-09-01 00:00:00
abstract：:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294703
更新日期：1995-12-01 00:00:00
abstract：:Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title：Current microbiology
pub_type： 新闻
doi：10.1007/s002849900261
更新日期：1997-11-01 00:00:00
abstract：:Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0600-x
更新日期：2007-08-01 00:00:00
abstract：:A Chinese HCV cDNA clone, designated as Q379, 379 nucleotides in length, was selected by recombinant immunoscreening from a random-primed Chinese HCV lambda gt11 library. DNA sequencing demonstrated that Q379 situated from positions 7314 to 7695 at NS5 region corresponded with the American prototype HCV nucleotide seq...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01571058
更新日期：1994-03-01 00:00:00
abstract：:The rates of urinary tract infection with multidrug-resistant (MDR) Escherichia coli have dramatically increased and the treatment of these infections with single and double antibiotic combinations became limited or ineffective. The present study aimed at finding effective antibiotic combinations against MDR uropathog...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01871-w
更新日期：2020-05-01 00:00:00
abstract：:Spontaneous white mutants from sectors of Streptomyces hygroscopicus 111-81 were isolated. The comparison of morphological, cultural, and biochemical properties of the mutants and ancestor showed the differences in colors of aerial, substrate mycelia, and sporulation. Changes in resistance to antibiotics and sensitivi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9165-1
更新日期：2008-08-01 00:00:00
abstract：:Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9815-y
更新日期：2011-03-01 00:00:00
abstract：:Strains of Vibrio cholerae O1 (Ogawa, Inaba) and non-O1 serogroups have been found to produce an elastolytic protease that can be detected on 0.3% elastin agar plates or in broth cultures. The elastase enzyme appears to be maximally expressed in late log phase (14-18 h postinoculation) and has optimum activity at a pH...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900421
更新日期：1999-08-01 00:00:00
abstract：:The adhesive property to the intestinal mucin of Bifidobacterium lactis LKM512, B. longum, B. breve, B. bifidum, B. adolescentis, B. infantis, Bacteroides vulgatus, Bacteroides distasonis, Eubacterium aerofaciens, Clostridium perfringens, Escherichia coli, and Lactobacillus acidophilus were examined. Adhesive rate of ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0087-4
更新日期：2002-03-01 00:00:00
abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1217-y
更新日期：2017-04-01 00:00:00
abstract：:Bradysia difformis is one of the most damaging pests in mushroom production in China. In this study, eight Bacillus thuringiensis strains were analyzed for insecticidal activity in B. difformis. The strain JW-1 showed the highest insecticidal activity against B. difformis larvae, but did not inhibit the mycelial growt...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02018-y
更新日期：2020-10-01 00:00:00
abstract：:It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the p...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0177-9
更新日期：2006-01-01 00:00:00
