Abstract：
:Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases in other organisms and is close to those from some filamentous fungi. Under nitrogen-starvation condition, the transcription of pcamy1 was accordingly upregulated or downregulated when soluble starch or glucose is sole carbon source. Addition of oxygen to nitrogen-limited media led to pcamy1 transcription and removal of glucose metabolic repression. The result indicated that the pcamy1 transcript was not only regulated by nutrients such as the carbon source but also by the cultivation environment, such as oxygen. This coordinate-regulatory model is likely common in P. chrysosporium. The expressed product of this gene in Escherichia coli could hydrolyze soluble starch, and its enzymatic activity was determined. As far as we know, this is the first report about cloning and expression study on the alpha-amylase in P. chrysosporium.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Wu B,Hu GK,Feng H,Wu JM,Zhang YZdoi
10.1007/s00284-006-0600-xsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
105-13issue
2eissn
0343-8651issn
1432-0991journal_volume
55pub_type
杂志文章abstract：:The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0056-5
更新日期：2012-03-01 00:00:00
abstract：:A Gram-positive, yellow pigmented actinobacterium, strain S2-48(T) was isolated from water sample collected from the river Brahmaputra, Assam, India and subjected to a polyphasic taxonomic study. Most of the physiological and biochemical properties, major fatty acids (C(15:0) Anteiso, iso C(16:0) and C(17:0) Anteiso),...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9822-z
更新日期：2011-03-01 00:00:00
abstract：:A broad-host-range vector that expresses a unique artificial RNA in Pseudomonas putida has been developed. This vector was derived from the plasmid pBBR1MCS and incorporates regulatory regions from the Escherichia coli ribosomal operon, rrnB. These include the promoters P1 and P2, and the terminators T1 and T2. The ge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910017
更新日期：2000-02-01 00:00:00
abstract：:Yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental pH. Previous results have indicated that secretion of the alkaline protease is under control of the pH signaling Pal/Rim pathway originally described in Aspergillus nidulans. Several Y. lipol...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0070-6
更新日期：2006-07-01 00:00:00
abstract：:An in vitro study was conducted to examine the metabolism of histidine (His) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP). Rumen microorganisms were collected from fistulated goats fed with lucerne cubes (Medicago sativa) and a concentrate mixture twice a day. Microbial susp...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010170
更新日期：2001-01-01 00:00:00
abstract：:The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0021-9
更新日期：2002-05-01 00:00:00
abstract：:A Gram-negative, non-motile, rod-shaped, aerobic bacterial strain, designated S7-3-3T, was isolated from apple orchard soil in Gyeongsangnam-do province, South Korea, and was characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S7-3-3T belong...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1434-z
更新日期：2018-06-01 00:00:00
abstract：:Mycoplasma penetrans invades HeLa cells and survives within them for prolonged periods of time. The intracellular distribution of M. penetrans within HeLa cells was studied utilizing the acidotropic dye LysoTracker (green), which permeates cell membranes and upon protonation remains trapped in acidic compartments. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4298-3
更新日期：2004-08-01 00:00:00
abstract：:The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0490-7
更新日期：2014-03-01 00:00:00
abstract：:In this study, vegetative insecticidal proteins vip3 genes from Bacillus thuringiensis strains were detected based on polymerase chain reaction-high resolution melt (PCR-HRM) analysis. A pair of primers was designed according to the conservative sequences in 150 bp region of the known vip3 subfamily. The 150 bp region...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0092-9
更新日期：2012-05-01 00:00:00
abstract：:Several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of Prevotella (formerly Bacteroides) ruminicola M384 and P. ruminicola B(1)4. The rates of breakdown of most peptides were similar in the rumen fl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01576265
更新日期：1993-06-01 00:00:00
abstract：:The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira inte...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9865-1
更新日期：2011-04-01 00:00:00
abstract：:A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900035
更新日期：1996-04-01 00:00:00
abstract：:Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-014-0651-3
更新日期：2014-11-01 00:00:00
abstract：:With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900272
更新日期：1998-01-01 00:00:00
abstract：:Interactions between Bacillus anthracis (B. anthracis) and host cells are of particular interest given the implications of anthrax as a biological weapon. Inhaled B. anthracis endospores encounter alveolar macrophages as the first line of defense in the innate immune response. Yet, the consequences of this interaction...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9654-x
更新日期：2010-12-01 00:00:00
abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0910-y
更新日期：2016-01-01 00:00:00
abstract：:The rates of urinary tract infection with multidrug-resistant (MDR) Escherichia coli have dramatically increased and the treatment of these infections with single and double antibiotic combinations became limited or ineffective. The present study aimed at finding effective antibiotic combinations against MDR uropathog...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01871-w
更新日期：2020-05-01 00:00:00
abstract：:Biofilm-forming bacteria Pseudomonas aeruginosa is a common pathogen in mechanically ventilated newborns, which can cause life-threatening infections. Alginate of mucoid Pseudomonas aeruginosa biofilms is considered an important virulence factor which contributes to the resistance to antibiotics. Traditionally, ambrox...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9142-8
更新日期：2008-07-01 00:00:00
abstract：:AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus ...
journal_title：Current microbiology
pub_type： 信件
doi：10.1007/s00284-013-0477-4
更新日期：2014-03-01 00:00:00
abstract：:A Chinese HCV cDNA clone, designated as Q379, 379 nucleotides in length, was selected by recombinant immunoscreening from a random-primed Chinese HCV lambda gt11 library. DNA sequencing demonstrated that Q379 situated from positions 7314 to 7695 at NS5 region corresponded with the American prototype HCV nucleotide seq...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01571058
更新日期：1994-03-01 00:00:00
abstract：:A total, 58 strains of Bacillus thuringiensis were isolated from soils of various regions in Korea. Serological tests showed that B. thuringiensis isolates represented 10 H serotypes, indicating a varied flora of B. thuringiensis. But the H serotypes did not have a significantly uneven distribution, ranging from 1 to ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900363
更新日期：1998-09-01 00:00:00
abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
abstract：:A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella and Spodoptera exigua was isolated from a Korean soil sample and characterized. The isolate, named B. thuringiensis K1, was determined to belong to ssp. kurstaki (H3a3b3c) type by an H antisera agglutination test and prod...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3755-0
更新日期：2002-10-01 00:00:00
abstract：:The effect of disodium fumarate (DF) on the ruminal fermentation profiles, the accumulation of lipopolysaccharide (LPS) and bioamines, and the composition of the ruminal bacterial community was investigated by in vitro rumen fermentation. The addition of DF increased the total gas production; the concentrations of pro...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1322-y
更新日期：2017-11-01 00:00:00
abstract：:A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty ac...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01802-9
更新日期：2020-01-01 00:00:00
abstract：:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0355-9
更新日期：2006-05-01 00:00:00
abstract：:Bipolaris oryzae is a filamentous ascomycetous fungus that causes brown leaf spot disease in rice. We isolated and characterized BLR2, a gene that encodes a putative blue-light regulator similar to Neurospora crassa white collar-2 (WC-2). The deduced amino acid sequence of the BLR2 showed significant homology to other...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9080-x
更新日期：2008-02-01 00:00:00
abstract：:The evolution of the symbiotic association with microbes allowed termites to decompose ingested lignocellulose from plant-derived substrates, including herbivore dung and soil humus. Representatives of the Syntermitinae (Termitidae) range in their feeding habits from wood and litter-feeding to humus-feeding species. H...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1567-0
更新日期：2018-12-01 00:00:00
abstract：:Rhodobacter sphaeroides is a bacterium that can produce hydrogen by interaction with hydrogenase and nitrogenase. We report a hydrogen production system using co-cultivation of hydrogenase in liquid medium and immobilized nitrogenase in Escherichia coli. The recombinant plasmid has been constructed to analyze the effe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0941-4
更新日期：2016-03-01 00:00:00
