Abstract：
:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize a wide range of biologically active natural compounds that are pharmacologically important. Twenty-nine plant-associated culturable bacteria were screened for the presence of the NRPS gene, of which seven bacterial NRPS gene fragments were successfully detected. According to our findings the presence of NRPS gene among the isolates does not always equate to their antagonistic ability. Phylogenetic analysis of the NRPS and 16S rRNA-encoding genes was used to predict HGT that may have occurred during gene evolution. The occurrence of HGT was demonstrated in the isolates (one inter-phylum and four intra-phyla) and was supported by phylogenetic analysis, mol% G+C content, and tetranucleotide usage pattern and codon usage frequency. Among the four intra-phyla HGT, one isolate showed inter-class HGT and three other isolates showed intra-class HGT.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Nongkhlaw FM,Joshi SRdoi
10.1007/s00284-015-0910-ysubject
Has Abstractpub_date
2016-01-01 00:00:00pages
1-11issue
1eissn
0343-8651issn
1432-0991pii
10.1007/s00284-015-0910-yjournal_volume
72pub_type
杂志文章abstract：:Burkholderia cepacia is an opportunistic pathogen that causes serious pulmonary infections in cystic fibrosis patients. Although several potential virulence factors-a protease, lipase, and two phospholipases C (one hemolytic and one nonhemolytic)-have been identified, only two, the protease and the lipase, have been d...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006793
更新日期：1999-04-01 00:00:00
abstract：:Because of heavy use of antimicrobials, antimicrobial resistance in bacteria has become of great concern. The effect of some widely used food additives such as sucralose on bacteria in the gut and the environment has also drawn increasing attention. In this study, we investigated the interaction between antimicrobials...
journal_title：Current microbiology
pub_type： 信件
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更新日期：2017-07-01 00:00:00
abstract：:The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4358-8
更新日期：2004-10-01 00:00:00
abstract：:It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the p...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0177-9
更新日期：2006-01-01 00:00:00
abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0095-4
更新日期：2002-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00314583
更新日期：1995-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0026-y
更新日期：2012-01-01 00:00:00
abstract：:A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% iden...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4467-z
更新日期：2006-04-01 00:00:00
abstract：:Two strains of the well-known insect pathogen and biopesticide, Bacillus thuringiensis (Bt), belonging to subspecies alesti (strain Bt5) and kurstaki (strain Bt213), were chosen for genetic characterization. The two strains belong to different serotypes and are currently classified into different subspecies, although ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0055-z
更新日期：2002-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0224-6
更新日期：2006-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02113-0
更新日期：2020-10-01 00:00:00
abstract：:Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900452
更新日期：1999-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0023-1
更新日期：2011-12-01 00:00:00
abstract：:Bacteria were isolated from rabbit faeces using equine caecal fluid as a growth medium. Two new isolates of the genus Streptococcus are described in terms of their biochemical properties. One of these has a 16S rRNA gene with 97.7%, and the other 98.5%, identity to Streptococcus thoraltensis. While S. thoraltensis has...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9619-0
更新日期：2010-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01772-y
更新日期：2019-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010159
更新日期：2000-12-01 00:00:00
abstract：:The regulation of the recA gene expression in the obligately anaerobic rumen bacterium Prevotella ruminicola was investigated by monitoring the recA-specific transcript level. P. ruminicola recA forms a monocistronic unit, but no SOS-box sequences resembling those of Escherichia coli or Bacillus subtilis can be identi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900306
更新日期：1998-05-01 00:00:00
abstract：:Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294281
更新日期：1995-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900325
更新日期：1998-06-01 00:00:00
abstract：:Biofilm-forming bacteria Pseudomonas aeruginosa is a common pathogen in mechanically ventilated newborns, which can cause life-threatening infections. Alginate of mucoid Pseudomonas aeruginosa biofilms is considered an important virulence factor which contributes to the resistance to antibiotics. Traditionally, ambrox...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9142-8
更新日期：2008-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900268
更新日期：1997-12-01 00:00:00
abstract：:Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900438
更新日期：1999-09-01 00:00:00
abstract：:Yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental pH. Previous results have indicated that secretion of the alkaline protease is under control of the pH signaling Pal/Rim pathway originally described in Aspergillus nidulans. Several Y. lipol...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0070-6
更新日期：2006-07-01 00:00:00
abstract：:To understand the mechanism of development of cross-resistance in food pathogen Bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. Pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1090-0
更新日期：2016-10-01 00:00:00
abstract：:The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900258
更新日期：1997-11-01 00:00:00
abstract：:To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9960-y
更新日期：2011-08-01 00:00:00
abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010186
更新日期：2001-02-01 00:00:00
abstract：:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation ti...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0006-8
更新日期：2002-04-01 00:00:00
abstract：:In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0317-6
更新日期：2013-06-01 00:00:00
abstract：:We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remot...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0435-1
更新日期：2014-01-01 00:00:00
