Abstract：
:High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation time had a profound effect on protease yields at all the concentrations of carbon and nitrogen used. The response surface raised and flattened with increase in time of incubation, and maximum protease production up to 1939 U/ml was obtained after 96 h of incubation. The model equation obtained was validated experimentally at maximum starch (15 mg/ml) and peptone (7.5 mg/ml) concentration with increased incubation time up to 144 h in the presence of minimum inoculum density (1%). An overall 2.6-fold increase in protease production was obtained as compared with mean observed response (750 U/ml) at zero level of all variables.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Puri S,Beg QK,Gupta Rdoi
10.1007/s00284-001-0006-8keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
286-90issue
4eissn
0343-8651issn
1432-0991journal_volume
44pub_type
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