Abstract:
:To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from Streptomyces hygroscopicus was used as the selection marker. Transformants which showed a significant increase in chitosanase production (approximately 2.1-fold than control) were obtained. Southern blot analysis indicated that most transformants had a single-copy T-DNA integration.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Liu H,Bao Xdoi
10.1007/s00284-008-9334-2subject
Has Abstractpub_date
2009-03-01 00:00:00pages
279-82issue
3eissn
0343-8651issn
1432-0991journal_volume
58pub_type
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