SACE_0012, a TetR-family transcriptional regulator, affects the morphogenesis of Saccharopolyspora erythraea.

Abstract:

:Saccharopolyspora erythraea, a mycelium-forming actinomycete, produces a clinically important antibiotic erythromycin. Extensive investigations have provided insights into erythromycin biosynthesis in S. erythraea, but knowledge of its morphogenesis remains limited. By gene inactivation and complementation strategies, the TetR-family transcriptional regulator SACE_0012 was identified to be a negative regulator of mycelium formation of S. erythraea A226. Detected by quantitative real-time PCR, the relative transcription of SACE_7115, the amfC homolog for an aerial mycelium formation protein, was dramatically increased in SACE_0012 mutant, whereas erythromycin biosynthetic gene eryA, a pleiotropic regulatory gene bldD, and the genes SACE_2141, SACE_6464, SACE_6040, that are the homologs to the sporulation regulators WhiA, WhiB, WhiG, were not differentially expressed. SACE_0012 disruption could not restore its defect of aerial development in bldD mutant, and also did not further accelerate the mycelium formation in the mutant of SACE_7040 gene, that was previously identified to be a morphogenesis repressor. Furthermore, the transcriptional level of SACE_0012 had not markedly changed in bldD and SACE_7040 mutant over A226. Taken together, these results suggest that SACE_0012 is a negative regulator of S. erythraea morphogenesis by mainly increasing the transcription of amfC gene, independently of the BldD regulatory system.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Yin X,Xu X,Wu H,Yuan L,Huang X,Zhang B

doi

10.1007/s00284-013-0410-x

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

647-51

issue

6

eissn

0343-8651

issn

1432-0991

journal_volume

67

pub_type

杂志文章
  • Mutants of the cyanobacterium anabaena sp. PCC 7120 altered in nitrate transport and reduction

    abstract::Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900452

    authors: Rai AK,Tiwari SP

    更新日期:1999-11-01 00:00:00

  • Complete Genome Sequences of Leclercia sp. W6 and W17 Isolated from a Gastric Cancer Patient.

    abstract::Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02075-3

    authors: Xu YY,Huang CJ,Xu L,Jiang XW,Xu XW,Xu XW

    更新日期:2020-10-01 00:00:00

  • Three new species of Aspergillus from Amazonian forest soil (Ecuador).

    abstract::From an undisturbed natural forest soil in Ecuador, three fungal strains of the genus Aspergillus were isolated. Based on molecular and morphological features they are described as three new species, named A. quitensis, A. amazonicus, and A. ecuadorensis. ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9178-9

    authors: Mares D,Andreotti E,Maldonado ME,Pedrini P,Colalongo C,Romagnoli C

    更新日期:2008-09-01 00:00:00

  • Effect of chlorhexidine on multi-species biofilms.

    abstract::With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900272

    authors: Wilson M,Patel H,Noar JH

    更新日期:1998-01-01 00:00:00

  • PCR methods for identification and specific detection of probiotic lactic acid bacteria.

    abstract::Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900082

    authors: Castellanos MI,Chauvet A,Deschamps A,Barreau C

    更新日期:1996-08-01 00:00:00

  • Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus.

    abstract::The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0352-7

    authors: Zheng D,Valdez-Cruz NA,Armengol G,Sevrez C,Munoz-Olaya JM,Yuan Z,Orduz S,Crickmore N

    更新日期:2007-01-01 00:00:00

  • pH and Not Cell Morphology Modulate pLIP2 Induction in the Dimorphic Yeast Yarrowia lipolytica.

    abstract::The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unc...

    journal_title:Current microbiology

    pub_type: 信件

    doi:10.1007/s00284-017-1207-0

    authors: Sassi H,Delvigne F,Kallel H,Fickers P

    更新日期:2017-03-01 00:00:00

  • Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing.

    abstract::Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) an...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-01922-7

    authors: Pati S,Maity S,Dash A,Jema S,Mohapatra S,Das S,Samantaray DP

    更新日期:2020-07-01 00:00:00

  • Multi-copy repression of Serratia marcescens nuclease expression by dinI.

    abstract::The dinI homolog of S. marcescens was cloned from a plasmid library by virtue of its ability to inhibit nuclease expression from the S. marcescens nucA gene integrated in the genome of E. coli. The S. marcescens DinI protein is 68% identical to DinI of E. coli. It has a similar effect on other SOS regulated genes and ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-001-0072-y

    authors: Berkmen M,Benedik MJ

    更新日期:2002-01-01 00:00:00

  • Purification and identification of a novel leucine aminopeptidase from Bacillus thuringiensis israelensis.

    abstract::A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-007-9004-9

    authors: Cahan R,Hetzroni E,Nisnevitch M,Nitzan Y

    更新日期:2007-11-01 00:00:00

  • Aureimonas leprariae sp. nov., Isolated from a Lepraria sp. Lichen.

    abstract::A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain YIM132180T, was isolated from a Lepraria sp. lichen collected from Pu'er, Yunnan Province, China. The strain grew at 15-35 °C (optimum, 25-28 °C), at 0-2% (w/v) NaCl (optimum, 0-1%) and at pH 6.0-9.0 (optimum, pH 7.0). The 16S rRNA ge...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01826-1

    authors: Zhang K,Jiang LQ,Wang LS,An DF,Lang L,Li GD,Wang XY,Shi SB,Li QY,Jiang CL,Jiang Y

    更新日期:2020-02-01 00:00:00

  • Isolation of Pasteurella haemolytica from grass, drinking water, and straw bedding used by sheep.

    abstract::Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...

    journal_title:Current microbiology

    pub_type: 新闻

    doi:10.1007/s002849900261

    authors: Burriel AR

    更新日期:1997-11-01 00:00:00

  • Animal Viruses Probe dataset (AVPDS) for microarray-based diagnosis and identification of viruses.

    abstract::AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus ...

    journal_title:Current microbiology

    pub_type: 信件

    doi:10.1007/s00284-013-0477-4

    authors: Yadav BS,Pokhriyal M,Vasishtha DP,Sharma B

    更新日期:2014-03-01 00:00:00

  • Restriction polymorphisms in the internal transcribed spacers and 5.8S rDNA of Saccharomyces.

    abstract::Polymorphisms in enzymatically amplified ribosomal DNA (rDNA) were examined in 18 strains of Saccharomyces. Restriction patterns generated from the region spanning the internal transcribed spacers (ITS) and the 5.8S rDNA produced two clusters corresponding to S. bayanus and S. cerevisiae. The type culture of S. carlsb...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01575857

    authors: Molina FI,Inoue T,Jong SC

    更新日期:1992-11-01 00:00:00

  • Periplasmic PQQ-dependent glucose oxidation in free-living and symbiotic rhizobia.

    abstract::The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacte...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002840010222

    authors: Bernardelli CE,Luna MF,Galar ML,Boiardi JL

    更新日期:2001-05-01 00:00:00

  • Thermophilin 110: a bacteriocin of Streptococcus thermophilus ST110.

    abstract::A screen of thermophilic lactic acid bacteria identified Streptococcus thermophilus strain ST110 as the putative producer of a bacteriocin with high level of activity against pediococci. Thermophilin 110 was isolated from culture supernatant after 16 h of growth and partially purified by a chloroform extraction proced...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-4540-7

    authors: Gilbreth SE,Somkuti GA

    更新日期:2005-09-01 00:00:00

  • Codon usage methods for horizontal gene transfer detection generate an abundance of false positive and false negative results.

    abstract::Bacteria acquire new DNA in a process known as horizontal gene transfer (HGT). To investigate the evolutionary impact of this transfer of DNA, various methods have been developed to detect past HGT events. For example, codon usage-based methods detect the presence of transferred genes by identifying atypical patterns ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-012-0205-5

    authors: Friedman R,Ely B

    更新日期:2012-11-01 00:00:00

  • F-like plasmid sequences in enteric bacteria of diverse origin, with implication of horizontal transfer and plasmid host range.

    abstract::Seventy-eight bacterial isolates from human, animal, and plant hosts, representing eight species of the family Enterobacteriaceae, were screened for F-like plasmid sequences. Of the examined human Escherichia coli strains, 28% harbored one or two of the three F-like, RepFI replication regions, while 35% of the examine...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-001-0039-7

    authors: Mulec J,Starcic M,Zgur-Bertok D

    更新日期:2002-04-01 00:00:00

  • A Bacillus thuringiensis isolate possessing a spore-associated filament.

    abstract::We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-003-4103-8

    authors: Rampersad J,Khan A,Ammons D

    更新日期:2003-10-01 00:00:00

  • Characteristics of the sigK Deletion Mutant from Bacillus thuringiensis var. israelensis Strain Bt-59.

    abstract::All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02150-9

    authors: Xu L,Han G,Fan X,Lv J,Zhang X,Peng Q,Zhang J,Xu J,Song F

    更新日期:2020-11-01 00:00:00

  • Culturable heterotrophic bacteria associated with healthy and bleached scleractinian Madracis decactis and the fireworm Hermodice carunculata from the remote St. Peter and St. Paul Archipelago, Brazil.

    abstract::We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remot...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0435-1

    authors: Moreira AP,Tonon LA,Pereira Cdo V,Alves N Jr,Amado-Filho GM,Francini-Filho RB,Paranhos R,Thompson FL

    更新日期:2014-01-01 00:00:00

  • Response of heat-shock protein (HSP) genes to temperature and salinity stress in the antarctic psychrotrophic bacterium Psychrobacter sp. G.

    abstract::Temperature and salinity fluctuations are two of the most important factors affecting the growth of polar bacteria. In an attempt to better understand the function of heat-shock proteins (HSPs) in the adaptive mechanisms of the Antarctic psychrotrophic bacterium Psychrobacter sp. G to such conditions, genes Hsp845, Hs...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0409-3

    authors: Che S,Song W,Lin X

    更新日期:2013-11-01 00:00:00

  • Analysis of the Endophytic Bacteria Community Structure and Function of Panax notoginseng Based on High-Throughput Sequencing.

    abstract::Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02068-2

    authors: Zhang C,Ma X,Zhu R,Liu Z,Gu M,Zhang J,Li Y,Xu Y,Zhu D

    更新日期:2020-10-01 00:00:00

  • iTRAQ-coupled 2D LC-MS/MS analysis of secreted proteome of HBV-replicating HepG2 cells: potential in biomarkers for prognosis of HCC.

    abstract::Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9608-3

    authors: Feng H,Wang M,Chen WN

    更新日期:2010-10-01 00:00:00

  • Enhancement in ex vivo phagocytic capacity of peritoneal leukocytes in mice by oral delivery of various lactic-acid-producing bacteria.

    abstract::Lactic-acid-producing bacteria (LABs) are known to have immunomodulating activity. In the current study, various LABs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. Viable but not heat-killed cells of Weissella kimchii strain PL9001, Lactobacillus fermentum strain ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-004-4377-5

    authors: Lee Y,Lee TS

    更新日期:2005-01-01 00:00:00

  • Immunological relationships of glutamine synthetases from marine and terrestrial enterobacteria.

    abstract::Glutamine synthetase (GS) fromBeneckea alginolytica strain 90 was purified to homogeneity as indicated by sodium dodecyl sulface polyacrylamide gel electrophoresis. The purified enzyme was injected into rabbits, and the antiserum, as well as previously prepared antiserum against the GS fromEscherichia coli, was used i...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF02602446

    authors: Baumann L,Baumann P

    更新日期:1980-07-01 00:00:00

  • Study of Molecular Karyotypes in Amoeboaphelidium protococcarum,the Endotrophic Parasite of Chlorophycean alga Scenedesmus

    abstract::On the basis of the pulsed field gel electrophoresis(PFGE) technique, molecular karyotypes in Amoeboaphelidiumprotococcarum (the endotrophic parasite of chlorophycean algae, whichcombines protozoon- and fungus-type characters) were determined. Molecularkaryotypes in the strains X1, X5, and X31, which differ in the hos...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900155

    authors: Pinevich A,Gromov B,Mamkaeva K,Nasonova E

    更新日期:1997-02-01 00:00:00

  • Development of a Novel Allele-Specific PCR Method for Rapid Assessment of Nervous Necrosis Virus Genotypes.

    abstract::Viral nervous necrosis infections are causing severe problems on aquaculture industry due to ecological and economic impacts. Their causal agent is nervous necrosis virus or nodavirus, which has been classified into four genotypes. Different genotypes correlate with differences in viral pathogenicity. Therefore, ratio...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0880-0

    authors: Toubanaki DK,Margaroni M,Karagouni E

    更新日期:2015-11-01 00:00:00

  • Characterization of Comamonas thiooxidans sp. nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti.

    abstract::Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9602-9

    authors: Narayan KD,Pandey SK,Das SK

    更新日期:2010-10-01 00:00:00

  • Correction to: Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.

    abstract::The authors would like to correct the errors in the publication of the original article. ...

    journal_title:Current microbiology

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s00284-020-01947-y

    authors: Neveling DP,Ahire JJ,Laubscher W,Rautenbach M,Dicks LMT

    更新日期:2020-08-01 00:00:00