iTRAQ-coupled 2D LC-MS/MS analysis of secreted proteome of HBV-replicating HepG2 cells: potential in biomarkers for prognosis of HCC.

Abstract:

:Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This work aims to investigate the secreted proteins in our recently established cell-based HBV replication system, using isobaric tags for relative and absolute quantitation (iTRAQ)-coupled 2D LC-MS/MS proteomics approach. Such proteins are reflective of early events of HBV infection and thus may have potential as prognostic biomarkers for development of liver diseases.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Feng H,Wang M,Chen WN

doi

10.1007/s00284-010-9608-3

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

280-4

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

61

pub_type

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