Abstract：
:Trioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with L-isoleucine as a unique starter unit, as well as many complex post-PKS tailoring enzymes and resistance and regulatory proteins. In this work, two regulatory genes, txn9 coding for a Streptomyces antibiotic regulatory protein family regulator and txn11 for a two-component response regulator, were revealed to be absolutely required for trioxacarcin production by individually inactivating all the six annotated regulatory genes in the txn cluster. Complementation assay suggested that these two activators do not have a regulatory cascade relationship. Moreover, transcriptional analysis showed that they activate 15 of the 28 txn operons, indicating that a complicated regulatory network is involved in the trioxacarcin production. Information gained from this study may be useful for improving the production of the highly potent trioxacarcin A.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Yang K,Qi LH,Zhang M,Hou XF,Pan HX,Tang GL,Wang W,Yuan Hdoi
10.1007/s00284-015-0868-9subject
Has Abstractpub_date
2015-10-01 00:00:00pages
458-64issue
4eissn
0343-8651issn
1432-0991journal_volume
71pub_type
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