Abstract:
:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors that contain all four structural genes, the chromosomal promoter ST(P2201) from Streptococcus thermophilus, and repA from the 2-kbp S. thermophilus plasmid pER8. The recombinant plasmid, pPC318, expressed and secreted active pediocin in Escherichia coli. Streptococcus thermophilus, Lactococcus lactis subsp. lactis, and Enterococcus faecalis were electrotransformed with pPC418, a modified vector fitted with an erythromycin resistance tracking gene. Pediocin was produced and secreted in each of the lactic acid bacteria, and production was stable for up to ten passages. The expression of pediocin in dairy fermentation microbes has important implications for bacteriocins as food preservatives in dairy products.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Coderre PE,Somkuti GAdoi
10.1007/s002849900462keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
295-301issue
5eissn
0343-8651issn
1432-0991pii
3076journal_volume
39pub_type
杂志文章abstract::The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3767-9
更新日期:2002-12-01 00:00:00
abstract::Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1284-0
更新日期:2017-09-01 00:00:00
abstract::The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0295-z
更新日期:2007-03-01 00:00:00
abstract::A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1553-6
更新日期:2018-11-01 00:00:00
abstract::Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and construct...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0500-9
更新日期:2014-04-01 00:00:00
abstract::All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02150-9
更新日期:2020-11-01 00:00:00
abstract::Magainin 2 is pore-forming antimicrobial peptide on lipid matrix of bacterial membrane, secreted from the skin of the African clawed frog Xenopus laevis. The aim of this study was to investigate a new concept for antibacterial mechanisms called bacterial apoptosis-like cell death. We examined the morphological changes...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0657-x
更新日期:2014-12-01 00:00:00
abstract::The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3812-8
更新日期:2003-02-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02201-1
更新日期:2020-11-01 00:00:00
abstract::Bacteria were isolated from rabbit faeces using equine caecal fluid as a growth medium. Two new isolates of the genus Streptococcus are described in terms of their biochemical properties. One of these has a 16S rRNA gene with 97.7%, and the other 98.5%, identity to Streptococcus thoraltensis. While S. thoraltensis has...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9619-0
更新日期:2010-10-01 00:00:00
abstract::The Gram-stain-negative, rod-shaped, aerobic strain, designated YC1039T, was isolated from a seamount northern Mariana Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC1039T was related to the genus Mesoflavibacter and had highest 16S rRNA gene sequence...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1500-6
更新日期:2018-09-01 00:00:00
abstract::The 5' untranslated region and the orf1 sequence from the cry2Aa1 operon from Bacillus thuringiensis subsp. kurstaki NRD-12 were sequenced and compared to that from strain HD-1. The start codon described in HD-1 does not yield in NRD-12 a protein of the expected size of 20 kDa, but a 10-amino acid peptide. A second, h...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006828
更新日期:1999-07-01 00:00:00
abstract::Rhodobacter sphaeroides is a bacterium that can produce hydrogen by interaction with hydrogenase and nitrogenase. We report a hydrogen production system using co-cultivation of hydrogenase in liquid medium and immobilized nitrogenase in Escherichia coli. The recombinant plasmid has been constructed to analyze the effe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0941-4
更新日期:2016-03-01 00:00:00
abstract::Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many species, including pigs, horses and humans. Biofilm formation is essential for pathogenesis, and the ability to resist antibiotic treatment results in difficult-to-treat and persistent infections. However, the ability o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0574-z
更新日期:2014-09-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02270-2
更新日期:2021-01-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4111-8
更新日期:2004-03-01 00:00:00
abstract::Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14-60 days a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0237-x
更新日期:2013-02-01 00:00:00
abstract::In past years, the significance of microbial resistance to biocides has increased. Twenty biocide-resistant bacterial strains were isolated from dental-unit water line biofilm. All strains resisted high biocide concentrations (up to 100 microg ml(-1)): sodium dodecyl sulphate, hydrogen peroxide, sodium hypochlorite, p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9200-2
更新日期:2008-10-01 00:00:00
abstract::We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...
journal_title:Current microbiology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Current microbiology
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journal_title:Current microbiology
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9692-4
更新日期:2011-01-01 00:00:00
abstract::Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02075-3
更新日期:2020-10-01 00:00:00
abstract::Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (C...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0554-z
更新日期:2007-07-01 00:00:00
abstract::This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9038-z
更新日期:2008-01-01 00:00:00
abstract::The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0490-7
更新日期:2014-03-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02113-0
更新日期:2020-10-01 00:00:00
abstract::The natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, enviro...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0023-1
更新日期:2011-12-01 00:00:00
abstract::We present a novel light-dependent metabolism of an aromatic compound (trans-cinnamate) that is assimilatory rather than dissimilatory. Light-dependent assimilation of trans-cinnamate was observed by both growing and resting cells of Rhodobacter sphaeroides OU5. Trans-cinnamate assimilation could be correlated with si...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0440-8
更新日期:2007-06-01 00:00:00
abstract::The archaeal diversity in salt pan sediment from Mumbai, India, was investigated by 16S rDNA-dependent molecular phylogeny. Small-subunit rRNA (16S rDNA) from salt pan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. Thirty-two unique phylotypes were o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9167-z
更新日期:2008-08-01 00:00:00