Abstract：
:Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (Ca(2+) and Mg(2+)) in order to investigate the effect of cations on the activity of these ostricacins on Gram-negative bacteria and Gram-positive bacteria. The radial diffusion assay method showed that both ostricacins were sensitive to the presence of cations. The divalent cations showed more antagonized effect on the activity against Gram-negative bacteria than the monovalent cations, as the ostricacins lost ability to inhibit bacterial growth at very low concentration (5 mM). When viewed in the context of other defensins activity, our data support a hypothesis that defensins' overall net positive charge determine the sensitivity to cations.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sugiarto H,Yu PLdoi
10.1007/s00284-006-0554-zsubject
Has Abstractpub_date
2007-07-01 00:00:00pages
36-41issue
1eissn
0343-8651issn
1432-0991journal_volume
55pub_type
杂志文章abstract：:The growth-inhibitory effect of 23 carbohydrate monoesters synthesized by lipases and proteases were assayed to obtain antibacterial agents that suppress the cell growth of Streptococcus mutans. Among the carbohydrate esters synthesized, galactose and fructose laurates showed the highest growth-inhibitory effect, whil...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010121
更新日期：2000-09-01 00:00:00
abstract：:Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...
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journal_title：Current microbiology
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更新日期：2010-07-01 00:00:00
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更新日期：2007-11-01 00:00:00
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更新日期：2015-02-01 00:00:00
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更新日期：2015-10-01 00:00:00
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更新日期：2007-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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journal_title：Current microbiology
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更新日期：2019-04-01 00:00:00
