Construction of plasmid vectors for screening replicons from gram-positive bacteria and their use as shuttle cloning vectors.

abstract：:The archaeal diversity in salt pan sediment from Mumbai, India, was investigated by 16S rDNA-dependent molecular phylogeny. Small-subunit rRNA (16S rDNA) from salt pan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. Thirty-two unique phylotypes were o...

journal_title：Current microbiology

authors： Ahmad N,Sharma S,Khan FG,Kumar R,Johri S,Abdin MZ,Qazi GN

更新日期：2008-08-01 00:00:00

abstract：:To date, a few works have performed a correlation of metabolic variables in bacteria; however specific correlations with these variables have not been reported. In this work, we included 36 human pathogenic bacteria and 18 non- or less-pathogenic-related bacteria and obtained all metabolic variables, including enzymes...

journal_title：Current microbiology

authors： Brambila-Tapia AJ,Poot-Hernández AC,Garcia-Guevara JF,Rodríguez-Vázquez K

更新日期：2016-06-01 00:00:00

abstract：:Nine structurally similar macrolide antibiotics were tested at a concentration of 0.5 microg/ml for their relative inhibitory effects on ribosome functions in Staphylococcus aureus cells. Eight of the compounds examined inhibited protein synthesis at this concentration. Seven of the nine compounds were also effective ...

journal_title：Current microbiology

authors： Champney WS,Tober CL,Burdine R

更新日期：1998-12-01 00:00:00

abstract：:A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...

journal_title：Current microbiology

authors： Stackebrandt E,Frühling A,Cousin S,Brambilla E,Lünsdorf H,Verbarg S

更新日期：2008-04-01 00:00:00

abstract：:Aminopeptidases (APN) may play a role in host colonization of M. gallinarum. Characterization of endogenous APN activity suggests that the leucine APN (LAP) of M. gallinarum is a metallo-aminopeptidase activated by Mn2+ and is present in the cytosol and possibly associated with the inner leaflet of the membrane. A 1.3...

journal_title：Current microbiology

authors： Wan X,Branton SL,Hanson LA,Pharr GT

更新日期：2004-01-01 00:00:00

abstract：:Several rhizobial strains possess the ability to modulate leguminous plants ethylene levels by producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase. While the effect of ACC deaminase has been studied in several rhizobia belonging to the Alphaproteobacteria class, not much is understood about its impa...

journal_title：Current microbiology

authors： Nascimento FX,Tavares MJ,Glick BR,Rossi MJ

更新日期：2018-08-01 00:00:00

abstract：:Fragments of Synechocystis-DNA driving salt-induced gene expression in E. coli were isolated with translational fusions to a 'lacZ gene. One fragment (fragment 19) showed a NaCl-dependent activation of betaGal expression with the maximum of a ninefold increase in enzyme activity. A similar induction was triggered by t...

journal_title：Current microbiology

authors： Milkowski C,Quinones A,Hagemann M

更新日期：1998-08-01 00:00:00

abstract：:Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...

journal_title：Current microbiology

authors： Martel MB,Letoublon R,Fevre M

更新日期：1996-10-01 00:00:00

abstract：:Avian pathogenic Escherichia coli (APEC) is one of the pathogens that most concerns the poultry industry worldwide due to the economic losses it can cause. APEC persistence and survival, both in the environment and in the host, may be a consequence of biofilm-producing capabilities. The aim of this study was to evalua...

journal_title：Current microbiology

authors： Rodrigues SV,Laviniki V,Borges KA,Furian TQ,Moraes HLS,Nascimento VP,Salle CTP

更新日期：2019-02-01 00:00:00

abstract：:Grasslands are suffering from long-term overgrazing because of the population inflation. Furthermore, nitrous oxide (N2O) is a major greenhouse gas that also depletes stratospheric ozone. However, the emission rate of grassland N2O and underlying mechanisms remained unclear under different grazing intensities. We cond...

journal_title：Current microbiology

authors： Du Y,Shu K,Guo X,Pengjin Z

更新日期：2019-05-01 00:00:00

abstract：:The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...

journal_title：Current microbiology

authors： Jensen B,Olsen J

更新日期：1996-09-01 00:00:00

abstract：:The interactions between the proteolytic X2L strain of Oenococcus oeni and the non-proteolytic 12p strain of Pediococcus pentosaceus were assayed. The characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. O. oeni showed poor cell grow...

journal_title：Current microbiology

authors： Fernández PA,de Nadra MC

更新日期：2006-02-01 00:00:00

abstract：:The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...

journal_title：Current microbiology

authors： Garcia M,Procópio L

更新日期：2020-06-01 00:00:00

abstract：:Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml(-1) in SF and 21.5 U g(-1) in SSF; these values were approximately ...

journal_title：Current microbiology

authors： De Azeredo LA,De Lima MB,Coelho RR,Freire DM

更新日期：2006-10-01 00:00:00

abstract：:Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...

journal_title：Current microbiology

authors： Gu Q,Yang ZH,Zhao DM,Zhang D,Wang Q,Ma LS,Zhu JH

更新日期：2017-09-01 00:00:00

abstract：:Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and posse...

journal_title：Current microbiology

authors： Yang H,Deng J,Yuan Y,Fan D,Zhang Y,Zhang R,Han B

更新日期：2015-02-01 00:00:00

abstract：:The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...

journal_title：Current microbiology

authors： Guerrini S,Mangani S,Granchi L,Vincenzini M

更新日期：2002-05-01 00:00:00

abstract：:A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...

journal_title：Current microbiology

authors： Sørensen SJ,Sørensen AH,Hansen LH,Oregaard G,Veal D

更新日期：2003-08-01 00:00:00

abstract：:The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...

journal_title：Current microbiology

authors： Chauvatcharin N,Vattanaviboon P,Switala J,Loewen PC,Mongkolsuk S

更新日期：2003-02-01 00:00:00

abstract：:In the last few years, numerous cases of multidrug-resistant Achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. To gain insights into the molecular mechanisms and mobile elements related to multidrug resistance in this bacterium, we studied 24 non-epidemiologi...

journal_title：Current microbiology

authors： Traglia GM,Almuzara M,Merkier AK,Adams C,Galanternik L,Vay C,Centrón D,Ramírez MS

更新日期：2012-12-01 00:00:00

abstract：:Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment w...

journal_title：Current microbiology

authors： Midhun SJ,Neethu S,Vysakh A,Radhakrishnan EK,Jyothis M

更新日期：2018-09-01 00:00:00

abstract：:Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cy...

journal_title：Current microbiology

authors： Jia J,Li Z,Cao J,Jiang Y,Liang C,Liu M

更新日期：2013-10-01 00:00:00

abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...

journal_title：Current microbiology

authors： Liu Z,Xie Y,Zhang X,Hu X,Li Y,Ding X,Xia L,Hu S

更新日期：2016-04-01 00:00:00

abstract：:Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus pr...

journal_title：Current microbiology

authors： Abdallah AI,Commander NJ,Woodward MJ,Spencer S,Hart CA,Winstanley C

更新日期：2003-04-01 00:00:00

abstract：:One of the main requirements for probiotics is their ability to survive during passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibito...

journal_title：Current microbiology

authors： Hakobyan L,Harutyunyan K,Harutyunyan N,Melik-Andreasyan G,Trchounian A

更新日期：2016-06-01 00:00:00

abstract：:This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the ons...

journal_title：Current microbiology

authors： Brzezinska MS,Lalke-Porczyk E,Kalwasińska A

更新日期：2012-12-01 00:00:00

abstract：:Multi-drug resistance and transfer of mobile genetic elements among enteric pathogens is being reported to have increased rapidly. Commensal Escherichia coli was previously known to acquire mobile genetic elements from other genus/species. E. coli is also capable of disseminating these elements containing antimicrobia...

journal_title：Current microbiology

authors： Devanga Ragupathi NK,Muthuirulandi Sethuvel DP,Gajendran R,Anandan S,Walia K,Veeraraghavan B

更新日期：2019-06-01 00:00:00

abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...

journal_title：Current microbiology

authors： Nongkhlaw FM,Joshi SR

更新日期：2016-01-01 00:00:00

abstract：:The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...

journal_title：Current microbiology

authors： Strohl WR,Larkin JM

更新日期：1978-01-01 00:00:00

abstract：:The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylor...

journal_title：Current microbiology

authors： Simala-Grant JL,Lam E,Keelan M,Taylor DE

更新日期：2004-07-01 00:00:00