Abstract:
:Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml(-1) in SF and 21.5 U g(-1) in SSF; these values were approximately 86% and 39% higher, respectively, than those obtained previously when yeast extract was used in place of CSL. The proteases, which belong to the serine and metalloproteinase classes, were active at high temperatures (55 degrees C to 90 degrees C) and over a wide range of pH values (5.0 to 10.0). Thus, these thermophilic proteases have shown interesting properties for industrial purposes. As far as we are concerned, this is the first contribution toward the microbial production of thermophilic proteases by a streptomycete using a low-cost medium composed of industrial poultry (FM) and corn processing by-products (CSL).
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
De Azeredo LA,De Lima MB,Coelho RR,Freire DMdoi
10.1007/s00284-006-0163-xsubject
Has Abstractpub_date
2006-10-01 00:00:00pages
335-9issue
4eissn
0343-8651issn
1432-0991journal_volume
53pub_type
杂志文章abstract::To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9426-7
更新日期:2009-09-01 00:00:00
abstract::This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9098-8
更新日期:2008-09-01 00:00:00
abstract::The regulation of the recA gene expression in the obligately anaerobic rumen bacterium Prevotella ruminicola was investigated by monitoring the recA-specific transcript level. P. ruminicola recA forms a monocistronic unit, but no SOS-box sequences resembling those of Escherichia coli or Bacillus subtilis can be identi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900306
更新日期:1998-05-01 00:00:00
abstract::A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9004-9
更新日期:2007-11-01 00:00:00
abstract::Interactions between Bacillus anthracis (B. anthracis) and host cells are of particular interest given the implications of anthrax as a biological weapon. Inhaled B. anthracis endospores encounter alveolar macrophages as the first line of defense in the innate immune response. Yet, the consequences of this interaction...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9654-x
更新日期:2010-12-01 00:00:00
abstract::Nine domestic wine samples collected from a Japanese winery were examined for the presence of fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3), as well as ochratoxin A (OTA) and ochratoxin B (OTB). Wine samples spiked with 13C-labeled internal standards (13C34-FB1 and 13C20-OTA) were diluted with phospha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02113-0
更新日期:2020-10-01 00:00:00
abstract::Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900319
更新日期:1998-06-01 00:00:00
abstract::A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900470
更新日期:1999-12-01 00:00:00
abstract::The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010199
更新日期:2001-03-01 00:00:00
abstract::Lysobacter enzymogenes OH11 produces heat-stable antifungal factor (HSAF) and lytic enzymes possessing antifungal activity. This study bio-prospected for other potential antifungal factors besides those above. The cells and extracellular metabolites of L. enzymogenes OH11 and the mutants ΔchiA, ΔchiB, ΔchiC, Δclp, Δpk...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1206-1
更新日期:2017-04-01 00:00:00
abstract::Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900438
更新日期:1999-09-01 00:00:00
abstract::The immunosuppressant leflunomide (LFM) inhibits the growth of cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of eukaryotic microorganism, Saccharomyces cerevisiae. As a first step to elucidate the molecular mechanism of LFM action in human, yeast gene which suppresses the an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010267
更新日期:2001-08-01 00:00:00
abstract::Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title:Current microbiology
pub_type: 新闻
doi:10.1007/s002849900261
更新日期:1997-11-01 00:00:00
abstract::A total of 216 bacterial strains were isolated from rice rhizospheric soils in Northern Thailand. The bacterial strains were initially tested for solubilization of inorganic phosphate, indole acetic acid (IAA) production, selected strains were then tested for optimized conditions for IAA production and whether these c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9674-6
更新日期:2011-01-01 00:00:00
abstract::Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00294521
更新日期:1995-01-01 00:00:00
abstract::An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02130-z
更新日期:2020-10-01 00:00:00
abstract::With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900272
更新日期:1998-01-01 00:00:00
abstract::Butterfly stain, common in Chilean tepa (Laureliopsis philippiana [Looser] Schodde) trees, appears in cross-section of the stem as a series of partially overlapping orange-brown arcs that in early stages resembles a butterfly. Bacterial isolates from stained tepa wood samples cultured under aerobic and anaerobic condi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910016
更新日期:2000-02-01 00:00:00
abstract::A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9095-3
更新日期:2008-04-01 00:00:00
abstract::A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910027
更新日期:2000-02-01 00:00:00
abstract::The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expre...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00294635
更新日期:1995-07-01 00:00:00
abstract::The first cloning and characterization of the gene katA, encoding the major catalase (KatA), from Xanthomonas is reported. A reverse genetic approach using a synthesized katA-specific DNA probe to screen a X. campestris pv. phaseoli genomic library was employed. A positively hybridizing clone designated pKat29 that co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3812-8
更新日期:2003-02-01 00:00:00
abstract::Iturin A is a very important cyclic lipopeptide produced by several B. subtilis strains and has large commercial and therapeutic application potentials but its production on industrial scale has not been realized yet. In the present study, we have observed that the strain ET-1 of Bacillus subtilis, a producer of Ituri...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01764-y
更新日期:2019-12-01 00:00:00
abstract::Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. dril...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0565-0
更新日期:2014-08-01 00:00:00
abstract::The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006784
更新日期:1999-03-01 00:00:00
abstract::Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8(T) under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemastertrade mark 2D Platinum software. The molecular masses of the majorit...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0221-4
更新日期:2007-01-01 00:00:00
abstract::Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians re...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1272-4
更新日期:2017-08-01 00:00:00
abstract::Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14-60 days a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0237-x
更新日期:2013-02-01 00:00:00
abstract::The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0021-9
更新日期:2002-05-01 00:00:00
abstract::A bacterium capable of utilizing p-cresol as sole source of carbon and energy was isolated from soil and identified as a Bacillus species. The organism also utilized phenol, o-cresol, m-cresol, 4-hydroxybenzoic acid, and gentisic acid as growth substrates. The organism degraded p-cresol to 4-hydroxybenzoic acid, which...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0309-x
更新日期:2006-12-01 00:00:00