Abstract:
:The immunosuppressant leflunomide (LFM) inhibits the growth of cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of eukaryotic microorganism, Saccharomyces cerevisiae. As a first step to elucidate the molecular mechanism of LFM action in human, yeast gene which suppresses the anti-proliferative effect when in increased copy number was cloned and designated MLF6 for multicopy suppressor of LFM sensitivity. DNA sequencing analysis revealed that the MLF6 gene is identical to the YPL244C gene which encodes a possible yeast homologue of human UDP-galactose transporter. The disruption of the MLF6 gene increased the sensitivity of yeast cells to the drug.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Fujimura Hdoi
10.1007/s002840010267keywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
93-5issue
2eissn
0343-8651issn
1432-0991journal_volume
43pub_type
杂志文章abstract::The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9239-0
更新日期:2008-12-01 00:00:00
abstract::pH affected significantly the growth and the glucose fermentation pattern of Propionibacterium microaerophilum. In neutral conditions (pH 6.5-7.5), growth and glucose fermentation rate (qs) were optimum producing propionate, acetate, CO(2), and formate [which together represented 90% (wt/wt) of the end products], and ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3839-x
更新日期:2003-02-01 00:00:00
abstract::One hundred and twenty-nine Vibrio anguillarum serogroup O2 strains were compared by slide agglutination and Western blotting for their lipopolysaccharide (LPS) structure. The strains showed six different LPS profiles, four different reaction patterns in Western blotting, and four different kinds of reaction in slide ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900141
更新日期:1997-01-01 00:00:00
abstract::Regulated secretory proteins are sorted via selective co-aggregation in eukaryotes. Cellobiase (C) of the filamentous fungus Termitomyces clypeatus remained co-aggregated with sucrase (S), and only one isoform of each of the enzymes was present in intra- and extracellular extracts. Kinetics of secretion of sucrase inc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-7001-y
更新日期:2002-07-01 00:00:00
abstract::Maize root colonization by two fluorescentPseudomonas strains M.3.1. and TR335, isolated respectively frommaize and tomato roots, were studied in hydroponic conditions. Each bacteriumwas inoculated separately, and three different colonization areas werestudied: nutrient solution, rhizoplane, and endorhizosphere. The t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900185
更新日期:1997-05-01 00:00:00
abstract::We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4186-2
更新日期:2004-04-01 00:00:00
abstract::Thymol (5-methyl-2-isopropylphenol) is a phenolic compound that is used to inhibit oral bacteria. Because little is known regarding the effects of this compound on ruminal microorganisms, the objective of this study was to determine the effects of thymol on growth and lactate production by the ruminal bacteria Strepto...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010145
更新日期:2000-11-01 00:00:00
abstract::Lamprey was considered to be one of the most basal jawless vertebrate representatives for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. Here we investigated the effect of the gut-derived Aeromonas on the lamprey leukocytes proteome using the label-free liquid chromatography-ta...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1468-2
更新日期:2018-07-01 00:00:00
abstract::Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9158-0
更新日期:2008-08-01 00:00:00
abstract::Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01949-w
更新日期:2020-08-01 00:00:00
abstract::Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0570-3
更新日期:2014-08-01 00:00:00
abstract::Fragments of Synechocystis-DNA driving salt-induced gene expression in E. coli were isolated with translational fusions to a 'lacZ gene. One fragment (fragment 19) showed a NaCl-dependent activation of betaGal expression with the maximum of a ninefold increase in enzyme activity. A similar induction was triggered by t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900347
更新日期:1998-08-01 00:00:00
abstract::In this study, the rumen was assessed for its potential to detoxify RDX using molecular microbial ecology as well as analytical chemistry techniques. Results indicated significant loss (P < 0.05) of RDX in <8-h post incubation, and qualitative LC-MS/MS analysis showed evidence for the formation of 1-NO-RDX (M-O + HCOO...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0144-1
更新日期:2012-08-01 00:00:00
abstract::A bacterium that tested positive with antibodies specific for Escherichia coli O157 was isolated from beef during routine screening procedures. The bacterium was identified as E. fergusonii by biochemical testing and partial sequencing of 16S rRNA. The isolate was tested for the presence of genes encoding Shiga toxins...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0447-6
更新日期:2006-06-01 00:00:00
abstract::A thermophilic bacterial strain GXN151 which could degrade Avicel efficiently was isolated and identified as Bacillus licheniformis. A genomic library of GXN151 was constructed and two novel endoglucanase genes designated cel9A and cel12A were isolated by screening the library on carboxylmethyl cellulase indicator pla...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4291-x
更新日期:2004-10-01 00:00:00
abstract::NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00298381
更新日期:1995-10-01 00:00:00
abstract::The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01898-4
更新日期:2020-06-01 00:00:00
abstract::The addition of increasing physiological concentrations of ammonia or amino acids had distinct effects on the growth and proteolytic activity of Streptococcus bovis JB1, Prevotella albensis, and Butyrivibrio fibrisolvens DSM3071. The growth of S. bovis and B. fibrisolvens was enhanced by NH(3) and AA, and that of P. a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010074
更新日期:2000-06-01 00:00:00
abstract::In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0317-6
更新日期:2013-06-01 00:00:00
abstract::During periods of drainage, both water stress and oxygen can cause damage to indigenous methanogens. In the present study, we evaluated the tolerance of seven methanogenic strains (Methanobrevibacter arboriphilicus, Methanobacterium formicicum, Methanococcus vannielii, Methanospirillum hungatei, Methanoculleus olentan...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9059-7
更新日期:2008-03-01 00:00:00
abstract::Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01570083
更新日期:1992-07-01 00:00:00
abstract::A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3978-0
更新日期:2003-08-01 00:00:00
abstract::Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage tite...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0984-6
更新日期:2016-05-01 00:00:00
abstract::A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain YIM132180T, was isolated from a Lepraria sp. lichen collected from Pu'er, Yunnan Province, China. The strain grew at 15-35 °C (optimum, 25-28 °C), at 0-2% (w/v) NaCl (optimum, 0-1%) and at pH 6.0-9.0 (optimum, pH 7.0). The 16S rRNA ge...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01826-1
更新日期:2020-02-01 00:00:00
abstract::A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonge...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1246-6
更新日期:2017-07-01 00:00:00
abstract::White pepper (Piper nigrum L.), a well-known spice, is the main pepper processing product in Hainan province, China. The solid-state method of fermentation can peel pepper in a highly efficient manner and yield high-quality white pepper. In the present study, we used next-generation sequencing to reveal the dynamic ch...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1242-x
更新日期:2017-06-01 00:00:00
abstract::The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0352-7
更新日期:2007-01-01 00:00:00
abstract::This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02212-y
更新日期:2020-12-01 00:00:00
abstract::Bacteria acquire new DNA in a process known as horizontal gene transfer (HGT). To investigate the evolutionary impact of this transfer of DNA, various methods have been developed to detect past HGT events. For example, codon usage-based methods detect the presence of transferred genes by identifying atypical patterns ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0205-5
更新日期:2012-11-01 00:00:00
abstract::We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remot...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0435-1
更新日期:2014-01-01 00:00:00