Label-Free Quantitative Proteomic Reveals Differentially Expressed Proteins in Aeromonas-Immunostimulated Leukocytes of Lampetra japonica.

Abstract:

:Lamprey was considered to be one of the most basal jawless vertebrate representatives for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. Here we investigated the effect of the gut-derived Aeromonas on the lamprey leukocytes proteome using the label-free liquid chromatography-tandem mass spectrometry for quantitative proteomics analysis. Significant difference was observed in the regulation of 34 out of 755 proteins in Aeromonas-immunized lamprey. 31 proteins were only identified in saline solution-immunized lamprey and 47 proteins were only identified in Aeromonas-immunized lamprey. Quantitative real-time polymerase chain reaction was used to validate the results of the proteomic analysis. The differentially expressed proteins were found to be associated with several different biological processes. The identification of leukocytes proteins essential for lamprey adaptive immune response induced by gut-derived Aeromonas strain could supply important information on lamprey-Aeromonas interactions and VLR-based adaptive immune signal pathways.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Li Y,Zhang W,Zuo Y,Zhu T,Pang Y,Li T,Li Q

doi

10.1007/s00284-018-1468-2

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

934-941

issue

7

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-018-1468-2

journal_volume

75

pub_type

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