Abstract：
:Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce the nitrate reductase (NR) gene from Chlorella vulgaris into NR-deficient Chlorella sorokiniana mutants, resulting in stable transformants. The stable transformants were able to grow on nitrate medium after repeated passages between selective and nonselective medium and exhibited inducible nitrate reductase activity comparable to that of wild-type cells. Southern analysis suggests homologous recombination occurs with insertion of the wild type gene into the mutated gene and that the genes of the two Chlorellaspecies used are very similar. Specific RNase protection assays, selecting for a poorly conserved region of the gene, identified the presence of the C. vulgaris NR transcript only in the transformed C. sorkiniana mutant and not in the mutant.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Dawson HN,Burlingame R,Cannons ACdoi
10.1007/s002849900268subject
Has Abstractpub_date
1997-12-01 00:00:00pages
356-62issue
6eissn
0343-8651issn
1432-0991pii
CM2633journal_volume
35pub_type
杂志文章abstract：:Next Generation Sequencing (NGS), using the Illumina® metabarcoding system, showed differences between biofilm communities on three degraded siliceous stone church façades in central Rio de Janeiro. Two church biofilms (on granite and augen gneiss) were dominated by Actinobacteria; the third (granite), surrounded by t...
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：2000-04-01 00:00:00
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journal_title：Current microbiology
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