Abstract:
:The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P2951T and characterized using taxono-genomics. Strain Marseille-P2951T is non-motile and non-spore forming and exhibits catalase and oxidase activities. Its 16S rRNA gene-based identification showed 98.5% identity with Ezakiella peruensis, the phylogenetically closest species. The major fatty acids are C18:1n9 (58%) and C16:0 (22%). With a 1,741,785 bp length, the G+C content of the genome is 36.69%. Of a total of 1657 genes, 1606 are protein-coding genes and 51 RNAs. Also, 1123 genes are assigned a putative function and 127 are ORFans. Phenotypic, phylogenetic, and genomics analyses revealed that strain Marseille-P2951T (=CSUR P2951 =DSM 103122) is distinct and represents a new species of the genus Ezakiella, for which the name Ezakiella massiliensis sp. nov. is proposed.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Diop K,Andrieu C,Michelle C,Armstrong N,Bittar F,Bretelle F,Fournier PE,Raoult D,Fenollar Fdoi
10.1007/s00284-017-1402-zsubject
Has Abstractpub_date
2018-04-01 00:00:00pages
456-463issue
4eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1402-zjournal_volume
75pub_type
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