Abstract:
:An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and 1H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL-1 of EPS-I as functional as approximately to 500 ± 5.2 µM of ascorbic acid) and Fe2+ metal ion chelation activity (EC50 = 405.6 µg mL-1) and hydroxyl radical scavenging activity (EC50 = 219.5 µg mL-1). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC50 = 267.3 µg mL-1) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Choudhuri I,Khanra K,Pariya P,Maity GN,Mondal S,Pati BR,Bhattacharyya Ndoi
10.1007/s00284-020-02130-zsubject
Has Abstractpub_date
2020-10-01 00:00:00pages
3125-3135issue
10eissn
0343-8651issn
1432-0991pii
10.1007/s00284-020-02130-zjournal_volume
77pub_type
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