Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells.

Abstract:

:An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and 1H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL-1 of EPS-I as functional as approximately to 500 ± 5.2 µM of ascorbic acid) and Fe2+ metal ion chelation activity (EC50 = 405.6 µg mL-1) and hydroxyl radical scavenging activity (EC50 = 219.5 µg mL-1). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC50 = 267.3 µg mL-1) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Choudhuri I,Khanra K,Pariya P,Maity GN,Mondal S,Pati BR,Bhattacharyya N

doi

10.1007/s00284-020-02130-z

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

3125-3135

issue

10

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-020-02130-z

journal_volume

77

pub_type

杂志文章
  • Diversity, Roles, and Biotechnological Applications of Symbiotic Microorganisms in the Gut of Termite.

    abstract::Termites are global pests and can cause serious damage to buildings, crops, and plantation forests. The symbiotic intestinal flora plays an important role in the digestion of cellulose and nitrogen in the life of termites. Termites and their symbiotic microbes in the gut form a synergistic system. These organism work ...

    journal_title:Current microbiology

    pub_type: 杂志文章,评审

    doi:10.1007/s00284-018-1502-4

    authors: Zhou J,Duan J,Gao M,Wang Y,Wang X,Zhao K

    更新日期:2019-06-01 00:00:00

  • Direct detection and quantification of horizontal gene transfer by using flow cytometry and gfp as a reporter gene.

    abstract::A new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3978-0

    authors: Sørensen SJ,Sørensen AH,Hansen LH,Oregaard G,Veal D

    更新日期:2003-08-01 00:00:00

  • A low-cost fermentation medium for thermophilic protease production by Streptomyces sp. 594 using feather meal and corn steep liquor.

    abstract::Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml(-1) in SF and 21.5 U g(-1) in SSF; these values were approximately ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0163-x

    authors: De Azeredo LA,De Lima MB,Coelho RR,Freire DM

    更新日期:2006-10-01 00:00:00

  • Effects of the dicarboxylic acids malate and fumarate on E. coli O157:H7 and Salmonella enterica typhimurium populations in pure culture and in mixed ruminal microorganism fermentations.

    abstract::The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9351-1

    authors: Nisbet DJ,Callaway TR,Edrington TS,Anderson RC,Krueger N

    更新日期:2009-05-01 00:00:00

  • Phylogenetic analyses and diterpenoid production by marine bacteria of the genus Saprospira.

    abstract::The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-004-4358-8

    authors: Mincer TJ,Spyere A,Jensen PR,Fenical W

    更新日期:2004-10-01 00:00:00

  • Field trial of biocides for control of Legionella in cooling towers.

    abstract::Legionella and amebae populations in 16 cooling towers were challenged with three commercially available biocide formulations. The active agents were: a chlorinated phenolic thioether (CPTE), bromo-nitro-propane-diol (BNPD), and bromo-chloro-dimethylhydantoin (BCD, in briquette form). The towers were dosed with these ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00296203

    authors: Bentham RH,Broadbent CR

    更新日期:1995-03-01 00:00:00

  • Immunological relationships of glutamine synthetases from marine and terrestrial enterobacteria.

    abstract::Glutamine synthetase (GS) fromBeneckea alginolytica strain 90 was purified to homogeneity as indicated by sodium dodecyl sulface polyacrylamide gel electrophoresis. The purified enzyme was injected into rabbits, and the antiserum, as well as previously prepared antiserum against the GS fromEscherichia coli, was used i...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF02602446

    authors: Baumann L,Baumann P

    更新日期:1980-07-01 00:00:00

  • Variable-number-of-tandem-repeats analysis of genetic diversity in Pasteuria ramosa.

    abstract::Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, a...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9104-1

    authors: Mouton L,Ebert D

    更新日期:2008-05-01 00:00:00

  • First Evidence of Intracellular Bacteria Cardinium in Thermophilic Mite Microzetorchestes emeryi (Acari: Oribatida): Molecular Screening of Bacterial Endosymbiont Species.

    abstract::We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01717-5

    authors: Konecka E,Olszanowski Z

    更新日期:2019-09-01 00:00:00

  • Contribution of Bacillus thuringiensis Spores to Toxicity of Purified Cry Proteins Towards Indianmeal Moth Larvae

    abstract::The influence of Bacillus thuringiensis subsp. kurstaki HD-1 spores upon the toxicity of purified Cry1Ab and Cry1C crystal proteins toward susceptible and BT-resistant Indianmeal moth (IMM, Plodia interpunctella) larvae was investigated. With susceptible larvae, HD-1 spores were toxic in the absence of crystal protein...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900074

    authors: Johnson DE,McGaughey WH

    更新日期:1996-07-01 00:00:00

  • Characterization of the DNA adenine 5'-GATC-3' methylase HpyIIIM from Helicobacter pylori.

    abstract::The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylor...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-004-4244-4

    authors: Simala-Grant JL,Lam E,Keelan M,Taylor DE

    更新日期:2004-07-01 00:00:00

  • Promotion of bacteriophage induction and recombination by the cloned Bordetella pertussis recA gene is copy-number dependent.

    abstract::Favre and coworkers (Favre et al., Biochimie 73:235-244, 1991) previously reported that the Bordetella pertussis recA gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in Escherichia coli RecA- mutants. Reexamination of these phenotypes demonstrated that, ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900029

    authors: Kuhl SA

    更新日期:1996-03-01 00:00:00

  • Biologic control ability of plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 isolated from milk.

    abstract::A plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 (B-30488) was isolated from cows' milk. Bacterial colonization and growth responses of different plant species after inoculation with B-30488 were evaluated in a controlled environment and in microplot assays. Survival and colonization of B-30488 in the p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0261-9

    authors: DasGupta SM,Khan N,Nautiyal CS

    更新日期:2006-12-01 00:00:00

  • Reliability of CARD-FISH procedure for enumeration of Archaea in deep-sea surficial sediments.

    abstract::The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-0056-5

    authors: Molari M,Manini E

    更新日期:2012-03-01 00:00:00

  • Biological, immunological, and genetic analysis of Bacillus thuringiensis isolated from granary in Korea.

    abstract::To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900336

    authors: Kim HS,Lee DW,Woo SD,Yu YM,Kang SK

    更新日期:1998-07-01 00:00:00

  • Development of a Semi-nested PCR-Based Method for Specific and Rapid Detection of Alternaria solani Causing Potato Early Blight in Soil.

    abstract::Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1284-0

    authors: Gu Q,Yang ZH,Zhao DM,Zhang D,Wang Q,Ma LS,Zhu JH

    更新日期:2017-09-01 00:00:00

  • Involvement of glycine and aspartate residues in the binding capacity of FAD in the NADH dehydrogenase from an alkaliphilic Bacillus.

    abstract::There is a region exhibiting a similarity of amino acid sequence near the carboxyl-terminal segment of each FAD-containing oxidoreductase. In this region, four amino acid residues-Thr, Ala, Gly, and Asp-are highly conserved. To determine the involvement of the four amino acid residues (Thr-469, Ala-476, Gly-478, and A...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3911-6

    authors: Shiraki M,Koyama N

    更新日期:2003-06-01 00:00:00

  • Magainin 2 induces bacterial cell death showing apoptotic properties.

    abstract::Magainin 2 is pore-forming antimicrobial peptide on lipid matrix of bacterial membrane, secreted from the skin of the African clawed frog Xenopus laevis. The aim of this study was to investigate a new concept for antibacterial mechanisms called bacterial apoptosis-like cell death. We examined the morphological changes...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0657-x

    authors: Lee W,Lee DG

    更新日期:2014-12-01 00:00:00

  • Properties of Antibiotic-Resistant Bacteria Isolated from Onsite Wastewater Treatment Plant in Relation to Biofilm Formation.

    abstract::The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected str...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1428-2

    authors: Jałowiecki Ł,Żur J,Chojniak J,Ejhed H,Płaza G

    更新日期:2018-05-01 00:00:00

  • Fatty Acids Composition and Biofilm Production of Attenuated Salmonella typhimurium dam and seqA Mutants After Exposure to UV-C.

    abstract::The goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqA mutants in Salmonella typhimurium bacterial cells and UV-C radiations. The exposure changed the fatty acids composition of dam and seqA/dam strai...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0858-y

    authors: Abdelwaheb C,Lobna M,Bouchra BA,Selma K,Ahmed L

    更新日期:2015-10-01 00:00:00

  • Identification of Cyt2Ba from a New Strain of Bacillus thuringiensis and Its Toxicity in Bradysia difformis.

    abstract::Bradysia difformis is one of the most damaging pests in mushroom production in China. In this study, eight Bacillus thuringiensis strains were analyzed for insecticidal activity in B. difformis. The strain JW-1 showed the highest insecticidal activity against B. difformis larvae, but did not inhibit the mycelial growt...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02018-y

    authors: Wang FF,Qu SX,Lin JS,Li HP,Hou LJ,Jiang N,Luo X,Ma L

    更新日期:2020-10-01 00:00:00

  • Microbiota Analysis of Eggshells in Different Areas and During Different Storage Time by Non-cultural Methods.

    abstract::This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02212-y

    authors: Shi Y,Wu S,Li W,Liu D,Ma G,Zhang Z,Yuan L,Li H,Zhang S,Mushtaq N,Xu J

    更新日期:2020-12-01 00:00:00

  • Horizontal Gene Transfer of the Non-ribosomal Peptide Synthetase Gene Among Endophytic and Epiphytic Bacteria Associated with Ethnomedicinal Plants.

    abstract::This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0910-y

    authors: Nongkhlaw FM,Joshi SR

    更新日期:2016-01-01 00:00:00

  • Microbiomes of Biofilms on Decorative Siliceous Stone: Drawbacks and Advantages of Next Generation Sequencing.

    abstract::Next Generation Sequencing (NGS), using the Illumina® metabarcoding system, showed differences between biofilm communities on three degraded siliceous stone church façades in central Rio de Janeiro. Two church biofilms (on granite and augen gneiss) were dominated by Actinobacteria; the third (granite), surrounded by t...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1257-3

    authors: Ogawa A,Celikkol-Aydin S,Gaylarde C,Baptista-Neto JA,Beech I

    更新日期:2017-07-01 00:00:00

  • Bombyx mori nucleopolyhedrovirus ORF101 encodes a budded virus envelope associated protein.

    abstract::Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0570-3

    authors: Chen H,Li M,Huang G,Mai W,Chen K,Zhou Y

    更新日期:2014-08-01 00:00:00

  • Changes in Clostridium (Clostridioides) difficile PCR-Ribotype Distribution and Antimicrobial Resistance in a German Tertiary Care Hospital Over the Last 10 Years.

    abstract::Clostridium (Clostridioides) difficile ribotype 027 (RT027) was detected in Germany for the first time in 2007 during an outbreak in the region of Trier, Rhineland-Palatinate and is today the most prevalent ribotype (RT) in Europe. We aimed to determine the changes in RT distribution and corresponding antimicrobial re...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01654-3

    authors: Piepenbrock E,Stelzer Y,Berger F,Jazmati N

    更新日期:2019-04-01 00:00:00

  • Spirosoma radiotolerans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil.

    abstract::A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0584-x

    authors: Lee JJ,Srinivasan S,Lim S,Joe M,Im S,Bae SI,Park KR,Han JH,Park SH,Joo BM,Park SJ,Kim MK

    更新日期:2014-09-01 00:00:00

  • Characterization and Complete Genome Analysis of Pseudomonas aeruginosa Bacteriophage vB_PaeP_LP14 Belonging to Genus Litunavirus.

    abstract::A lytic Pseudomonas aeruginosa phage vB_PaeP_LP14 belonging to the family Podoviridae was isolated from infected mink. The microbiological characterization revealed that LP14 was stable at 40 to 50 °C and stable over a broad range of pH (5 to 12). The latent period was 5 min, and the burst size was 785 pfu/infected ce...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02011-5

    authors: Shi X,Zhao F,Sun H,Yu X,Zhang C,Liu W,Pan Q,Ren H

    更新日期:2020-09-01 00:00:00

  • Quorum sensing signalling and biofilm formation of brewery-derived bacteria, and inhibition of signalling by natural compounds.

    abstract::Bacteria use quorum sensing signalling in various functions, e.g. while forming biofilms, and inhibition of this signalling could be one way to control biofilm formation. The aim of this study was to evaluate the production of signalling molecules and its correlation with the biofilm formation capability of bacteria i...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0627-3

    authors: Priha O,Virkajärvi V,Juvonen R,Puupponen-Pimiä R,Nohynek L,Alakurtti S,Pirttimaa M,Storgårds E

    更新日期:2014-11-01 00:00:00

  • Dietary supplementation of usnic acid, an antimicrobial compound in lichens, does not affect rumen bacterial diversity or density in reindeer.

    abstract::Reindeer (Rangifer tarandus tarandus) may include large proportions of lichens in their winter diet. These dietary lichens are rich in phenolic secondary compounds, the most well-known being the antimicrobial usnic acid. Previous studies have shown that reindeer host rumen bacteria resistant to usnic acid and that usn...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0534-7

    authors: Glad T,Barboza P,Mackie RI,Wright AD,Brusetti L,Mathiesen SD,Sundset MA

    更新日期:2014-06-01 00:00:00