Abstract：
:To isolate a naturally occurring novel Bacillus thuringiensis strain, we investigated the distribution, toxicity, morphology, H serotype, and gene type of B. thuringiensis from residue samples of granary in Korea. A total of 163 B. thuringiensis isolates out of 411 samples producing spore and crystal were obtained. In toxicity tests, 80% of all isolates were toxic to lepidoptera, and 12% were not toxic to any of tested insects. And dipteran-active and lepidopteran/dipteran-active isolates were rare (2% and 6%, respectively). 152 B. thuringiensis isolates produced typical rhomboidal crystals, and the remainder produced parasporal inclusions with various morphologies. Serological test showed that B. thuringiensis isolates in granary represented 12 H serotypes, indicating varied distribution of B. thuringiensis. Of these, the serotype 3ab predominated, followed by the serotype 7 and 4ac. B. thuringiensis isolates of the serotype 3ab, 4ac, 5ab, 7, 8ab, 9, and 23 were toxic to lepidoptera, and the serotype 8bd, 12, 18, and 20ac were nontoxic, while 14 isolates were untypable by 33 B. thuringiensis H antisera. The frequency of toxicity against lepidoptera and diptera was primarily highly toxic. PCR analysis using cryI gene type-specific primers showed that cryIA(b) genes are frequently found and cryIE gene exists in only one isolate. Analysis of B. thuringiensis crystals and plasmid DNAs indicated a diversity of crystal and gene types.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Kim HS,Lee DW,Woo SD,Yu YM,Kang SKdoi
10.1007/s002849900336subject
Has Abstractpub_date
1998-07-01 00:00:00pages
52-7issue
1eissn
0343-8651issn
1432-0991journal_volume
37pub_type
杂志文章abstract：:All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02150-9
更新日期：2020-11-01 00:00:00
abstract：:Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0600-x
更新日期：2007-08-01 00:00:00
abstract：:Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, patho...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01647-2
更新日期：2019-04-01 00:00:00
abstract：:Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title：Current microbiology
pub_type： 新闻
doi：10.1007/s002849900261
更新日期：1997-11-01 00:00:00
abstract：:There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suita...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1429-1
更新日期：2018-06-01 00:00:00
abstract：:Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01739-z
更新日期：2019-12-01 00:00:00
abstract：:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0085-2
更新日期：2007-09-01 00:00:00
abstract：:Corynebacterium glutamicum, a Gram-positive soil bacterium belonging to the mycolic acids-containing actinomycetes, is able to use the lignin degradation products ferulate, vanillate, and protocatechuate as sole carbon sources. The gene cluster responsible for vanillate catabolism was identified and characterized. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4531-8
更新日期：2005-07-01 00:00:00
abstract：:Lactic and butyric acid production by bacterial flocs in a continuous culture obeyed different physiological constraints. The butyric acid rate of production was constant and independent of the growth rate [0.012 +/- 0.001 gBUT/(L.h)], whereas lactic fermentation occurred only beyond a critical growth rate (0.25 +/- 0...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4169-3
更新日期：2004-03-01 00:00:00
abstract：:We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01717-5
更新日期：2019-09-01 00:00:00
abstract：:The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01577379
更新日期：1993-04-01 00:00:00
abstract：:Flagella occur on many prokaryotes, which primarily propel cells to move from detrimental to favorable environments. A variety of species-specific flagellation patterns have been identified. Although it is presumed that for each of these flagellated microorganisms, an evolutionarily fixed flagellation pattern is favor...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01999-0
更新日期：2020-08-01 00:00:00
abstract：:Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900082
更新日期：1996-08-01 00:00:00
abstract：:In the last few years, numerous cases of multidrug-resistant Achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. To gain insights into the molecular mechanisms and mobile elements related to multidrug resistance in this bacterium, we studied 24 non-epidemiologi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0213-5
更新日期：2012-12-01 00:00:00
abstract：:Cyanide has been proved to be degraded by Klebsiella oxytoca. In order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-DE) electrophoresis approach and MALDI-TOF-MS allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mM cyanid...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9529-1
更新日期：2010-03-01 00:00:00
abstract：:With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900272
更新日期：1998-01-01 00:00:00
abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0095-4
更新日期：2002-08-01 00:00:00
abstract：:Clostridium (Clostridioides) difficile ribotype 027 (RT027) was detected in Germany for the first time in 2007 during an outbreak in the region of Trier, Rhineland-Palatinate and is today the most prevalent ribotype (RT) in Europe. We aimed to determine the changes in RT distribution and corresponding antimicrobial re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01654-3
更新日期：2019-04-01 00:00:00
abstract：:Rhodobacter sphaeroides is a bacterium that can produce hydrogen by interaction with hydrogenase and nitrogenase. We report a hydrogen production system using co-cultivation of hydrogenase in liquid medium and immobilized nitrogenase in Escherichia coli. The recombinant plasmid has been constructed to analyze the effe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0941-4
更新日期：2016-03-01 00:00:00
abstract：:We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9179-8
更新日期：2008-08-01 00:00:00
abstract：:Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment w...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1506-0
更新日期：2018-09-01 00:00:00
abstract：:Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900107
更新日期：1996-10-01 00:00:00
abstract：:Predation by Bdellovibrio-like organisms (BLOs) results in bacterial community succession in aquatic ecosystems. The effects of nutrient loading on the distribution and phylogeny of BLOs remain largely unknown. To this end, we present our findings on BLO diversity from four north-Indian lakes that are variable in thei...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9385-z
更新日期：2009-07-01 00:00:00
abstract：:A total of 168 Campylobacter strains (154 C. jejuni and 14 C. coli) isolated from human clinical samples and chicken meat were typed using Penner serotyping, randomly amplified polymorphic DNA (RAPD), and pulsed-field gel electrophoresis (PFGE) with four restriction enzymes (Sac II, Sal I, Sma I, Kpn I). The 168 strai...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-4037-6
更新日期：2003-11-01 00:00:00
abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01575864
更新日期：1992-11-01 00:00:00
abstract：:Seventy-eight bacterial isolates from human, animal, and plant hosts, representing eight species of the family Enterobacteriaceae, were screened for F-like plasmid sequences. Of the examined human Escherichia coli strains, 28% harbored one or two of the three F-like, RepFI replication regions, while 35% of the examine...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0039-7
更新日期：2002-04-01 00:00:00
abstract：:Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900413
更新日期：1999-02-01 00:00:00
abstract：:Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010081
更新日期：2000-07-01 00:00:00
abstract：:Lipids contribute significantly to the pathogenesis of fungal infectious diseases and an understanding of lipid metabolism occurring in fungal pathogens can help the development of more efficient antifungal therapeutic strategies. In this study, the effect of culture age on the distribution of fatty acids among differ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9052-1
更新日期：2008-02-01 00:00:00
abstract：:This report presents results from a study on the bacterial diversity of cigarette brands collected from military personnel during the U.S. Army's investigation of a series of cases of acute eosinophilic pneumonitis in military personnel deployed in Operation Iraqi Freedom. Eight species of Bacillus, including five new...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4491-z
更新日期：2005-07-01 00:00:00
