Abstract：
:Rhodobacter sphaeroides is a bacterium that can produce hydrogen by interaction with hydrogenase and nitrogenase. We report a hydrogen production system using co-cultivation of hydrogenase in liquid medium and immobilized nitrogenase in Escherichia coli. The recombinant plasmid has been constructed to analyze the effect of hydrogen production on the expression of hupSL hydrogenase and nifHDK nitrogenase isolated from R. sphaeroides. All recombinant E. coli strains were cultured anaerobically, and cells for nitrogenase were immobilized in agar gel, whereas cells for hydrogenase were supplemented on the nitrogenase agar gel. The hupSL hydrogenase has been observed to enhance hydrogen production and hydrogenase activity under co-culture with nifHDK nitrogenase. The maximum hydrogen production has been obtained at an agar gel concentration and a cell concentration for co-culture of 2 % and 6.4 × 10(8) CFU. Thus, co-culture of hupSL hydrogenase and nifHDK nitrogenase provides a promising route for enhancing the hydrogen production and hydrogenase activity.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Lee HJ,Sekhon SS,Kim YS,Park JY,Kim YH,Min Jdoi
10.1007/s00284-015-0941-4subject
Has Abstractpub_date
2016-03-01 00:00:00pages
242-7issue
3eissn
0343-8651issn
1432-0991pii
10.1007/s00284-015-0941-4journal_volume
72pub_type
杂志文章abstract：:Pseudomonas putida E41 isolated from root interior of Sida hermaphrodita (grown on a field contaminated with heavy metals) showed high biosurfactant activity. In this paper, we describe data from mass spectrometry and genome analysis, to improve our understanding on the phenotypic properties of the strain. Supernatant...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01757-x
更新日期：2019-11-01 00:00:00
abstract：:Saccharopolyspora erythraea, a mycelium-forming actinomycete, produces a clinically important antibiotic erythromycin. Extensive investigations have provided insights into erythromycin biosynthesis in S. erythraea, but knowledge of its morphogenesis remains limited. By gene inactivation and complementation strategies,...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0410-x
更新日期：2013-12-01 00:00:00
abstract：:The aim of the research was (i) to evaluate white and cream strains of A. bisporus yields when challenged or not with the pathogen L. fungicola, (ii) to identify the more aggressive pathogen isolates, and (iii) to develop a diagrammatic scale of spot symptoms on infected mushrooms. The experiment was carried out using...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02232-8
更新日期：2020-12-01 00:00:00
abstract：:A novel actinomycete, designated strain LAM7112T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20-45 °C (optimum: 35 °C), pH 5.0-10.0 (optimum: 7.0) and in the presence of 0-10.0% (optimum: 3.0%) (w/v) NaCl. The ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02192-z
更新日期：2020-11-01 00:00:00
abstract：:The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47 degrees C. Wild-type cell sur...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910056
更新日期：2000-04-01 00:00:00
abstract：:Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial Sau3A digestion were cloned into the unique BamHI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC)promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900171
更新日期：1997-04-01 00:00:00
abstract：:A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-020-02296-6
更新日期：2021-01-02 00:00:00
abstract：:This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the ons...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0230-4
更新日期：2012-12-01 00:00:00
abstract：:This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was exa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9438-3
更新日期：2009-09-01 00:00:00
abstract：:In the present study, EMA (ethidium monoazide) treatment was applied to a silty-sand reference soil prior to DNA extraction to enable a differentiation between dead and living cells. For this purpose, a reference soil was spiked with Listeria monocytogenes cells or cell equivalents, respectively. With the purpose of e...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01772-y
更新日期：2019-12-01 00:00:00
abstract：:The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region we...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0578-8
更新日期：2014-09-01 00:00:00
abstract：:Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9602-9
更新日期：2010-10-01 00:00:00
abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1217-y
更新日期：2017-04-01 00:00:00
abstract：:Bacterial FtsE gene codes for the ATP-binding protein, FtsE, which in complex with the transmembrane protein, FtsX, participates in diverse cellular processes. Therefore, regulated expression of FtsE and FtsX might be critical to the human pathogen, Mycobacterium tuberculosis, under stress conditions. Although ftsX ge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9897-1
更新日期：2011-05-01 00:00:00
abstract：:Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900319
更新日期：1998-06-01 00:00:00
abstract：:Two strains of a novel basidiomycetous yeast species were isolated from the gut of wood-boring larvae collected in the Baotianman Nature Reserve, the central China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) regions showed that these yeasts belong ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0203-7
更新日期：2012-11-01 00:00:00
abstract：:The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45 degrees C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37 degrees C. The invertase activity was significantly inhibited by bivalent meta...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910036
更新日期：2000-03-01 00:00:00
abstract：:Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9572-y
更新日期：2010-07-01 00:00:00
abstract：:Corynebacterium glutamicum, a Gram-positive soil bacterium belonging to the mycolic acids-containing actinomycetes, is able to use the lignin degradation products ferulate, vanillate, and protocatechuate as sole carbon sources. The gene cluster responsible for vanillate catabolism was identified and characterized. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4531-8
更新日期：2005-07-01 00:00:00
abstract：:The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010111
更新日期：2000-09-01 00:00:00
abstract：:The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0295-z
更新日期：2007-03-01 00:00:00
abstract：:A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonge...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1246-6
更新日期：2017-07-01 00:00:00
abstract：:Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studie...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3740-7
更新日期：2002-10-01 00:00:00
abstract：:Pseudomonas aeruginosa is a serious pathogen involved in nosocomial infections. Its pathogenicity is owed to rich production of virulence factors (VIFs) regulated by several complex hierarchical signal systems depending on environmental conditions, medium composition, and the presence of certain active compounds in it...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0441-7
更新日期：2007-04-01 00:00:00
abstract：:The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900092
更新日期：1996-09-01 00:00:00
abstract：:The facultative anaerobe Pantoea sp. strain A-13, isolated from ornithogenic soil of Dewart Island (Frazier Islands), Antarctica, produced glycolipid biosurfactants when grown on n-paraffins or kerosene as the sole source of carbon and energy. Hemolysis of erythrocytes, growth inhibition of Bacillus subtilis, and thin...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0345-6
更新日期：2007-02-01 00:00:00
abstract：:Magnetotactic bacteria (MTB) are capable of synthesizing nano-sized, intracellular membrane-bound magnetosomes. To learn more about the genetic factors involved in magnetosome formation, transposon mutagenesis was carried out by conjugation using a hyperactive mariner transposon to obtain nonmagnetic mutants of Magnet...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0131-6
更新日期：2012-07-01 00:00:00
abstract：:Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9347-x
更新日期：2009-04-01 00:00:00
abstract：:Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02270-2
更新日期：2021-01-01 00:00:00
abstract：:The Bacillus subtilis pur operon repressor (PurR) has a PRPP (5-phosphoribosyl 1-pyrophosphate) binding motif at residues 199-211. Two PurR PRPP binding region mutations (D203A and D204A) were constructed, and the effects on binding of repressor to the pur operon control site in vitro and on regulation of pur operon e...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010091
更新日期：2000-07-01 00:00:00
