Abstract:
:Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studied the effect of low phosphate concentration and acid pH on the APase activities of S. marcescens. In a low phosphate medium, some strains of S. marcescens synthesize two different types of APases, a constitutive (CAPase) and an inducible (IAPase). Both the CAPase and IAPase isoenzymes completely lost their enzyme activities at pH 2.3, within 10 min of incubation at 0 degrees C. Acid-treated IAPase isoenzymes I, II, III, and IV solutions when adjusted to pH 7.8 showed recovery of 70%, 52%, 72%, and 60% of the lost activities, respectively. When the pH of the CAPase reaction mixture was raised to pH 7.8, the enzyme activity regained only 5% of its initial activity. Variations in protein concentration also affected the pH-dependent reversible changes of the IAPase activity. The higher the protein concentration, the faster the inactivation of enzyme activity observed at acidic pH at 0 degrees C. Conversely, the lower the protein concentration, the higher the rate of reactivation of enzyme activity observed for IAPase at alkaline pH. Protein interaction studies revealed a lack of similarity between CAPase and IAPase, suggesting separate genetic origin of these potentially virulent genes of S. marcescens.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Bhatti AR,Alvi A,Walia S,Chaudhry GRdoi
10.1007/s00284-002-3740-7keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
245-9issue
4eissn
0343-8651issn
1432-0991journal_volume
45pub_type
杂志文章abstract::Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and construct...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0500-9
更新日期:2014-04-01 00:00:00
abstract::Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9386-y
更新日期:2009-06-01 00:00:00
abstract::In this study, the rumen was assessed for its potential to detoxify RDX using molecular microbial ecology as well as analytical chemistry techniques. Results indicated significant loss (P < 0.05) of RDX in <8-h post incubation, and qualitative LC-MS/MS analysis showed evidence for the formation of 1-NO-RDX (M-O + HCOO...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0144-1
更新日期:2012-08-01 00:00:00
abstract::Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both mo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900163
更新日期:1997-03-01 00:00:00
abstract::We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remot...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0435-1
更新日期:2014-01-01 00:00:00
abstract::Biofouling and microbiologically influenced corrosion are processes of material deterioration that originate from the attachment of microorganisms as quickly as the material is immersed in a nonsterile environment. Stainless steels, despite their wide use in different industries and as appliances and implant materials...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0193-4
更新日期:2006-12-01 00:00:00
abstract::Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02270-2
更新日期:2021-01-01 00:00:00
abstract::Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9104-1
更新日期:2008-05-01 00:00:00
abstract::The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0490-7
更新日期:2014-03-01 00:00:00
abstract::Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01949-w
更新日期:2020-08-01 00:00:00
abstract::Subsurface Wastewater Infiltration System (SWIS) is a sewage ecological treatment technology with low investment, energy consumption, and operating cost. SWIS soil contains a large variety of microorganisms. The metabolic process and production of microorganisms are an important basis for qualitatively describing the ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01830-5
更新日期:2020-02-01 00:00:00
abstract::Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0627-z
更新日期:2007-06-01 00:00:00
abstract::Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0133-8
更新日期:2006-03-01 00:00:00
abstract::The influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from Lactobacillus fermentum CRL 251 was studied. Stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; sc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900118
更新日期:1996-11-01 00:00:00
abstract::Leucoagaricus gongylophorus, the fungus cultured by the leaf-cutting ant Atta sexdens, produces polysaccharidases that degrade leaf components by generating nutrients believed to be essential for ant nutrition. We evaluated pectinase, amylase, xylanase, and cellulase production by L. gongylophorus in laboratory cultur...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0431-1
更新日期:2006-07-01 00:00:00
abstract::The lipid modification of membrane proteins was investigated in Acholeplasma laidlawii by metabolic labeling and by chemical analysis. A S-glycerylcysteine residue was identified from membrane proteins and we reported the strong preference for saturated acyl chains into the lipid modification. Differential release of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010332
更新日期:2001-12-01 00:00:00
abstract::Broad variation among anoxygenic reaction centers makes it essential to consider a wide variety when considering the origins of photosynthesis. The photosynthetic core antenna domain in the gene pshA from Heliophilum fasciatum was sequenced doubling the number of core sequences available from heliobacteria. The sequen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4221-3
更新日期:2004-06-01 00:00:00
abstract::The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9107-y
更新日期:2008-05-01 00:00:00
abstract::Lactic-acid-producing bacteria (LABs) are known to have immunomodulating activity. In the current study, various LABs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. Viable but not heat-killed cells of Weissella kimchii strain PL9001, Lactobacillus fermentum strain ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4377-5
更新日期:2005-01-01 00:00:00
abstract::The adhesive property to the intestinal mucin of Bifidobacterium lactis LKM512, B. longum, B. breve, B. bifidum, B. adolescentis, B. infantis, Bacteroides vulgatus, Bacteroides distasonis, Eubacterium aerofaciens, Clostridium perfringens, Escherichia coli, and Lactobacillus acidophilus were examined. Adhesive rate of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0087-4
更新日期:2002-03-01 00:00:00
abstract::A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9469-9
更新日期:2009-11-01 00:00:00
abstract::Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/pl00006756
更新日期:1998-03-01 00:00:00
abstract::A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1553-6
更新日期:2018-11-01 00:00:00
abstract::Pseudomonas aeruginosa is a leading cause of hospital-acquired infections and difficult to treat due to acquired-resistance to multiple antibiotics. A pair of strains, M38100A and M38100B, previously identified from a single clinical isolate of P. aeruginosa was investigated to understand phenotypic and genotypic char...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9570-0
更新日期:2010-07-01 00:00:00
abstract::Colonial aggregation of Microcystis plays a key role in bloom formation. Limited studies have been reported about effects of environmental factors on the aggregation of Microcystis. Calcium is an important chemical element in water system. In this study, we investigated the effects of a low- (0.015 g l⁻¹) and a high-c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9762-7
更新日期:2011-02-01 00:00:00
abstract::A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase ch...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900035
更新日期:1996-04-01 00:00:00
abstract::Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. dril...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0565-0
更新日期:2014-08-01 00:00:00
abstract::Mycobacteria are widely present in diverse aquatic habitats, where they can survive for months or years while some species can even proliferate. The resistance of different mycobacterial species to disinfection methods like chlorination or ozonation could result in their presence in the final tap water of consumers. I...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0427-1
更新日期:2013-12-01 00:00:00
abstract::Iturin A is a very important cyclic lipopeptide produced by several B. subtilis strains and has large commercial and therapeutic application potentials but its production on industrial scale has not been realized yet. In the present study, we have observed that the strain ET-1 of Bacillus subtilis, a producer of Ituri...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01764-y
更新日期:2019-12-01 00:00:00
abstract::The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0243-z
更新日期:2013-01-01 00:00:00