Isolation and characterization of transcription signal sequences from Streptococcus thermophilus.

Abstract:

:Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial Sau3A digestion were cloned into the unique BamHI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC)promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with transcription-activation activity were isolated from chloramphenicol-resistant (CmR) EC transformants. A promoterless Streptomyces antibioticus melanin biosynthesis operon (melC) was inserted immediately downstream from the ST sequence to identify DNA with strong promoter activity. Several ST transcription-activation sequences, termed STPs, were isolated and subcloned, and their nucleic acid sequences determined. The -10 and -35 consensus sequences were identified in these putative ST promoters. Detailed analysis of STP3306 sequence data revealed two partial open reading frames (ORFs) with high degrees of homology to prokaryotic GTP-binding protein and DNA repair enzyme, thus providing valuable information for further study on DNA maintenance in this important lactic acid bacterium.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Solaiman DK,Somkuti GA

doi

10.1007/s002849900171

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

216-9

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

34

pub_type

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