Cys92, Cys101, Cys197, and Cys203 are crucial residues for coordinating the iron-sulfur cluster of RhdA from Acidithiobacillus ferrooxidans.

abstract：:Corynebacterium diphtheriae strains expressed variation in hydrophobic characteristics dependent on the method used. Results of single assays are not a reliable representation of C. diphtheriae hydrophobicity. All 12 strains adhered to polystyrene surfaces; three showed spontaneous aggregation (SA) in Trypticase Soy B...

journal_title：Current microbiology

authors： Mattos-Guaraldi AL,Formiga LC,Andrade AF

更新日期：1999-01-01 00:00:00

abstract：:Candida galli strain PGO6 isolated from oil-contaminated water is the first isolated yeast strain which is capable to form vanillin and vanillic acid during isoeugenol biotransformation. The products were confirmed by thin-layer chromatography (TLC), changes in the UV absorption pattern and high-performance liquid chr...

journal_title：Current microbiology

authors： Ashengroph M,Nahvi I,Zarkesh-Esfahani H,Momenbeik F

更新日期：2011-03-01 00:00:00

abstract：:Magnetotactic bacteria (MTB) are capable of synthesizing nano-sized, intracellular membrane-bound magnetosomes. To learn more about the genetic factors involved in magnetosome formation, transposon mutagenesis was carried out by conjugation using a hyperactive mariner transposon to obtain nonmagnetic mutants of Magnet...

journal_title：Current microbiology

authors： Bo T,Wang K,Ge X,Chen G,Liu W

更新日期：2012-07-01 00:00:00

abstract：:Campylobacter coli cells are characterized by a comma, or spiral shape, and a single polar flagellum. Here we report stable spontaneous changes in morphology to an aflagellated straight rod. The ability to colonize the chick cecum was lost. The atypical cells were compared with the original C. coli 67 culture from whi...

journal_title：Current microbiology

authors： Ziprin RL,Hume ME,Andrews K,Droleskey RE,Harvey RB,Sheffield CL

更新日期：2005-09-01 00:00:00

abstract：:A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% iden...

journal_title：Current microbiology

authors： Kang SH,Cho KK,Bok JD,Kim SC,Cho JS,Lee PC,Kang SK,Lee HG,Woo JH,Lee HJ,Lee SC,Choi YJ

更新日期：2006-04-01 00:00:00

abstract：:Amino acid residues in the active site of quinoline 2-oxidoreductase (Qor) that are deemed important for substrate binding and turnover were replaced by site-directed mutagenesis. The apparent k(cat) values for quinoline were reduced 2.4-, 38-, 40-, and 199-fold in the protein variants QorA259G, QorW331G, QorV373A, an...

journal_title：Current microbiology

authors： Purvanov V,Fetzner S

更新日期：2005-04-01 00:00:00

abstract：:The cluster of genes encoding components of the progenitor botulinum neurotoxin complex has been mapped and cloned in Clostridium botulinum type G strain ATCC 27322. Determination of the nucleotide sequence of the region has revealed open reading frames encoding nontoxic components of the complex, upstream of the gene...

journal_title：Current microbiology

authors： Bhandari M,Campbell KD,Collins MD,East AK

更新日期：1997-10-01 00:00:00

abstract：:The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...

journal_title：Current microbiology

authors： Zhang H,Fan H,Lu C

更新日期：2010-12-01 00:00:00

abstract：:A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldo...

journal_title：Current microbiology

authors： Palacios GC,Timmons BC,Eskew EK,Solórzano F,Mattingly SJ

更新日期：2003-10-01 00:00:00

abstract：:This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...

journal_title：Current microbiology

authors： Shi Y,Wu S,Li W,Liu D,Ma G,Zhang Z,Yuan L,Li H,Zhang S,Mushtaq N,Xu J

更新日期：2020-12-01 00:00:00

abstract：:In eukaryotes, loss of cytoplasmic ribosomal proteins (RPs) results in a reduced growth rate and other phenotypic defects. The ability to transition from a unicellular budding yeast to a filamentous form is very important for biofilm formation and virulence in Candida albicans. Our recent study found that loss of the ...

journal_title：Current microbiology

authors： Lu H,Xiong J,Shang Q,Jiang Y,Cao Y

更新日期：2016-06-01 00:00:00

abstract：:Streptococcus bovis is commonly present in the rumen, but strains of S. bovis have also occasionally been isolated from human blood or fecal samples. Studies were undertaken with 16s rRNA gene sequences and DNA hybridizations to define the genetic relationships between these two groups of strains. Ruminal strains were...

journal_title：Current microbiology

authors： Nelms LF,Odelson DA,Whitehead TR,Hespell RB

更新日期：1995-11-01 00:00:00

abstract：:Iturin A is a very important cyclic lipopeptide produced by several B. subtilis strains and has large commercial and therapeutic application potentials but its production on industrial scale has not been realized yet. In the present study, we have observed that the strain ET-1 of Bacillus subtilis, a producer of Ituri...

journal_title：Current microbiology

authors： Ambrico A,Trupo M,Magarelli RA

更新日期：2019-12-01 00:00:00

abstract：:Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5...

journal_title：Current microbiology

authors： Alqublan H,Seleem MN,Boyle SM,Sriranganathan N

更新日期：2010-04-01 00:00:00

abstract：:Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...

journal_title：Current microbiology

authors： Monnerat R,Masson L,Brousseau R,Pusztai-Carey M,Bordat D,Frutos R

更新日期：1999-09-01 00:00:00

abstract：:The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. The gene of ferred...

journal_title：Current microbiology

authors： Zeng J,Huang X,Liu Y,Liu J,Qiu G

更新日期：2007-12-01 00:00:00

abstract：:Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...

journal_title：Current microbiology

authors： Gu Q,Yang ZH,Zhao DM,Zhang D,Wang Q,Ma LS,Zhu JH

更新日期：2017-09-01 00:00:00

abstract：:To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and...

journal_title：Current microbiology

authors： Liu H,Bao X

更新日期：2009-03-01 00:00:00

abstract：:Immobilized cells of Enterobacter agglomerans, able to reduce azo dyes enzymatically, were used as a biocatalyst for the decolorization of synthetic medium containing the toxic azo dye methyl red (MR). This bacterial strain exhibits high ability to completely decolorize 100 mg/L of MR after only 6 h of incubation unde...

journal_title：Current microbiology

authors： Moutaouakkil A,Zeroual Y,Dzayri FZ,Talbi M,Lee K,Blaghen M

更新日期：2004-02-01 00:00:00

abstract：:Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce t...

journal_title：Current microbiology

authors： Dawson HN,Burlingame R,Cannons AC

更新日期：1997-12-01 00:00:00

abstract：:Adenosine 5'-phosphosulfate (APS) reductase is a key enzyme involved in the pathways of sulfate reduction and sulfide oxidation in the biological sulfur cycle. In this study, the gene of APS reductase from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the soluble protein was purified by ...

journal_title：Current microbiology

authors： Zheng C,Zhang Y,Liu Y,Wu A,Xia L,Zeng J,Liu J,Qiu G

更新日期：2009-06-01 00:00:00

abstract：:The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and Sinorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacte...

journal_title：Current microbiology

authors： Bernardelli CE,Luna MF,Galar ML,Boiardi JL

更新日期：2001-05-01 00:00:00

abstract：:A Gram-negative, yellow pigmented bacterium designated strain CA5(T) that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence a...

journal_title：Current microbiology

authors： Hunter WJ,Manter DK

更新日期：2011-02-01 00:00:00

abstract：:Lectins LI and LII, localized on the surface of the nitrogen-fixing soil bacterium Bacillus polymyxa 1460, were shown to possess proteolytic activity. A relationship was found between the proteolytic and hemagglutinating activities of the lectins. Blocking of hemagglutinating activity with specific carbohydrate hapten...

journal_title：Current microbiology

authors： Mel'nikova UY,Karpunina LV,Ostakhina NV,Ignatov VV

更新日期：2000-10-01 00:00:00

abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...

journal_title：Current microbiology

authors： Porteous LA,Armstrong JL,Seidler RJ,Watrud LS

更新日期：1994-11-01 00:00:00

abstract：:Aminopeptidases (APN) may play a role in host colonization of M. gallinarum. Characterization of endogenous APN activity suggests that the leucine APN (LAP) of M. gallinarum is a metallo-aminopeptidase activated by Mn2+ and is present in the cytosol and possibly associated with the inner leaflet of the membrane. A 1.3...

journal_title：Current microbiology

authors： Wan X,Branton SL,Hanson LA,Pharr GT

更新日期：2004-01-01 00:00:00

abstract：:The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira inte...

journal_title：Current microbiology

authors： Hartwig DD,Seixas FK,Cerqueira GM,McBride AJ,Dellagostin OA

更新日期：2011-04-01 00:00:00

abstract：:Pseudomonas seleniipraecipitans grows in the presence of high levels of selenite and selenate and reduces both oxyanions to elemental selenium (Se(0)), a property that may make P. seleniipraecipitans useful as an inoculant for biobarriers designed to remove selenite or selenate from ground or surface waters. An earlie...

journal_title：Current microbiology

authors： Hunter WJ

更新日期：2014-07-01 00:00:00

abstract：:The influence of Bacillus thuringiensis subsp. kurstaki HD-1 spores upon the toxicity of purified Cry1Ab and Cry1C crystal proteins toward susceptible and BT-resistant Indianmeal moth (IMM, Plodia interpunctella) larvae was investigated. With susceptible larvae, HD-1 spores were toxic in the absence of crystal protein...

journal_title：Current microbiology

authors： Johnson DE,McGaughey WH

更新日期：1996-07-01 00:00:00

abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...

journal_title：Current microbiology

authors： Karunakaran T,Gunasekaran P

更新日期：1992-11-01 00:00:00