Abstract：
:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with the restriction endonuclease PalI. The extraction method was used to obtain DNA from a variety of plants, bacteria, and fungi including Gossypium hirsucum (cotton), Pseudomonas, Bacillus, Streptomyces, and Colletotrichum. Up to 100 micrograms DNA/g (wet weight) of soil and 400 micrograms DNA/g of plant material were recovered. Restriction endonuclease analysis patterns of amplified rDNA from pure microbial cultures and plant species contained three to five different DNA fragments. Amplified rDNA of mixed population DNA extracts from soil samples, digested with the restriction endonuclease PalI, contained 12-20 DNA fragments, appearing as sample "fingerprints." Results from eight environmental soil samples that were analyzed suggest that the amplified rDNA fingerprints can be used to help characterize the genetic and biological diversity of the microbial populations in these samples.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Porteous LA,Armstrong JL,Seidler RJ,Watrud LSdoi
10.1007/BF01577445subject
Has Abstractpub_date
1994-11-01 00:00:00pages
301-7issue
5eissn
0343-8651issn
1432-0991journal_volume
29pub_type
杂志文章abstract：:Thirty-one Listeria strains were tested for sensitivity to four class IIa bacteriocins, namely, enterocin A, mesentericin Y105, divercin V41, and pediocin AcH, and to nisin A. Class IIa bacteriocins displayed surprisingly similar antimicrobial patterns ranging from highly susceptible to fully resistant strains, wherea...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010081
更新日期：2000-07-01 00:00:00
abstract：:Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encodin...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4288-5
更新日期：2004-09-01 00:00:00
abstract：:Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8(T) under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemastertrade mark 2D Platinum software. The molecular masses of the majorit...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0221-4
更新日期：2007-01-01 00:00:00
abstract：:All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02150-9
更新日期：2020-11-01 00:00:00
abstract：:Actinomycetes are a wide group of Gram positive prokaryotes, the soil being their most characteristic habitat, where they play important ecological functions. Their immense biotechnological potential as producers of bioactive molecules of great commercial and industrial interest is exemplified by most antibiotics for ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02030-2
更新日期：2020-09-01 00:00:00
abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
abstract：:During periods of drainage, both water stress and oxygen can cause damage to indigenous methanogens. In the present study, we evaluated the tolerance of seven methanogenic strains (Methanobrevibacter arboriphilicus, Methanobacterium formicicum, Methanococcus vannielii, Methanospirillum hungatei, Methanoculleus olentan...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9059-7
更新日期：2008-03-01 00:00:00
abstract：:Pasteurella haemolytica was isolated from three of 18 grass samples and four of 18 water samples collected from two grazing fields occupied by sheep. This microorganism was also isolated from three of nine straw bedding samples collected from a pen housing ewes affected by mastitis caused by P. haemolytica. The same e...
journal_title：Current microbiology
pub_type： 新闻
doi：10.1007/s002849900261
更新日期：1997-11-01 00:00:00
abstract：:A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty ac...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01802-9
更新日期：2020-01-01 00:00:00
abstract：:Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main fl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1094-9
更新日期：2016-10-01 00:00:00
abstract：:Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0627-z
更新日期：2007-06-01 00:00:00
abstract：:A total of 216 bacterial strains were isolated from rice rhizospheric soils in Northern Thailand. The bacterial strains were initially tested for solubilization of inorganic phosphate, indole acetic acid (IAA) production, selected strains were then tested for optimized conditions for IAA production and whether these c...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9674-6
更新日期：2011-01-01 00:00:00
abstract：:Despite their ubiquity, relatively few bacteriophages have been characterized. Here, we set out to explore Caulobacter bacteriophages (caulophages) in the rhizosphere and characterized Kronos, the first caulophage isolated from the rhizosphere. Kronos is a member of the Siphoviridae family since it has a long flexible...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01656-1
更新日期：2019-05-01 00:00:00
abstract：:The dinI homolog of S. marcescens was cloned from a plasmid library by virtue of its ability to inhibit nuclease expression from the S. marcescens nucA gene integrated in the genome of E. coli. The S. marcescens DinI protein is 68% identical to DinI of E. coli. It has a similar effect on other SOS regulated genes and ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0072-y
更新日期：2002-01-01 00:00:00
abstract：:A 4.5-kilobase DNA fragment from Buchnera aphidicola, the endosymbiont of the aphid Schizaphis graminum, was cloned and sequenced. On the basis of homology to Escherichia coli, the following genes were found in the order listed: aroH-thrS-infC-rpmI-rplT. AroH corresponds to the E. coli tryptophan-inhibited 3-deoxy-D-a...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00295507
更新日期：1995-05-01 00:00:00
abstract：:The immunosuppressant leflunomide (LFM) inhibits the growth of cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of eukaryotic microorganism, Saccharomyces cerevisiae. As a first step to elucidate the molecular mechanism of LFM action in human, yeast gene which suppresses the an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010267
更新日期：2001-08-01 00:00:00
abstract：:Staphylococcus aureus causes a wide range of suppurative infections in humans and animals. Due to its high virulence, ability to adopt various environmental conditions, and acquired multiple drug resistance, treatment of such infections has become difficult. Therefore, there is an immense need to develop alternate dru...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9939-8
更新日期：2011-07-01 00:00:00
abstract：:We report on the first characterization of the culturable heterotrophic bacteria of the scleractinian Madracis decactis. In addition, we characterized the culturable bacteria associated with the fireworm Hermodice carunculata, observed predating partially bleached coral colonies. Our study was carried out in the remot...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0435-1
更新日期：2014-01-01 00:00:00
abstract：:Previous studies have established that psyllids (Hemiptera, Psylloidea) contain primary endosymbionts, designated as Carsonella ruddii, which cospeciate with the psyllid host. This association appears to be the consequence of a single infection of a psyllid ancestor with a bacterium. Some psyllids may have additional ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010138
更新日期：2000-10-01 00:00:00
abstract：:Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Binding of the bacterium to the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified flgC as a potential binding mutant of...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0521-z
更新日期：2014-05-01 00:00:00
abstract：:Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this st...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1241-y
更新日期：2017-06-01 00:00:00
abstract：:A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910027
更新日期：2000-02-01 00:00:00
abstract：:Bradysia difformis is one of the most damaging pests in mushroom production in China. In this study, eight Bacillus thuringiensis strains were analyzed for insecticidal activity in B. difformis. The strain JW-1 showed the highest insecticidal activity against B. difformis larvae, but did not inhibit the mycelial growt...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02018-y
更新日期：2020-10-01 00:00:00
abstract：:Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9585-6
更新日期：2010-08-01 00:00:00
abstract：:In this study, fatty-acid content and patterns were analyzed in order to distinguish Nostoc flagelliforme, an edible terrestrial cyanobacterium, from other Nostoc species and representatives typical of its close neighbors (genera Anabaena, Microcystis, and Synechococcus). According to the Kenyon-Murata classification ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4059-8
更新日期：2003-12-01 00:00:00
abstract：:Pseudomonas seleniipraecipitans grows in the presence of high levels of selenite and selenate and reduces both oxyanions to elemental selenium (Se(0)), a property that may make P. seleniipraecipitans useful as an inoculant for biobarriers designed to remove selenite or selenate from ground or surface waters. An earlie...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0555-2
更新日期：2014-07-01 00:00:00
abstract：:Tropical plants represent hotspots of endophytic fungal species diversity. Based on culture-dependent methods, we evaluated the endophytic fungal communities in leaves of three plant species found in the Brazilian Atlantic Rainforest: Begonia fischeri, Begonia olsoniae, and Begonia venosa. These species are found in t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1400-1
更新日期：2018-04-01 00:00:00
abstract：:The interactions between the proteolytic X2L strain of Oenococcus oeni and the non-proteolytic 12p strain of Pediococcus pentosaceus were assayed. The characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. O. oeni showed poor cell grow...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0079-x
更新日期：2006-02-01 00:00:00
abstract：:Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9386-y
更新日期：2009-06-01 00:00:00
abstract：:This study was undertaken to identify exopolysaccharide-producing bacteria gathered from 18 hypersaline habitats. Phenotypic studies performed with 134 isolates revealed the majority of them to be Gram-negative rods with respiratory metabolism, belonging to the genus Halomonas. A numerical analysis of the 114 phenotyp...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4208-0
更新日期：2004-05-01 00:00:00
