Characterization and virulence of hemolysin III from Vibrio vulnificus.

abstract：:Interactions between Bacillus anthracis (B. anthracis) and host cells are of particular interest given the implications of anthrax as a biological weapon. Inhaled B. anthracis endospores encounter alveolar macrophages as the first line of defense in the innate immune response. Yet, the consequences of this interaction...

journal_title：Current microbiology

authors： Porasuphatana S,Cao GL,Tsai P,Tavakkoli F,Huwar T,Baillie L,Cross AS,Shapiro P,Rosen GM

更新日期：2010-12-01 00:00:00

abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...

journal_title：Current microbiology

authors： Gaur R,Alam SI,Kamboj DV

更新日期：2017-04-01 00:00:00

abstract：:Pigment from the endophyte Burkholderia sp. WYAT7 isolated from the medicinal plant Artemisia nilagirica (Clarke) Pamp. was extracted. The antibacterial efficacy of the crude pigment Y was assessed as a source of antibiotic against both Gram-positive and Gram-negative bacterial pathogens. The pigment Y exhibited a sig...

journal_title：Current microbiology

authors： Ashitha A,Radhakrishnan EK,Mathew J

更新日期：2020-09-01 00:00:00

abstract：:The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. The biofilm formation is strongly influenced by the availability of nutrients and environmental conditions, and particularly enhanced in poor minimal essen...

journal_title：Current microbiology

authors： Zhou Q,Feng X,Zhang Q,Feng F,Yin X,Shang J,Qu H,Luo Q

更新日期：2012-07-01 00:00:00

abstract：:A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest ...

journal_title：Current microbiology

authors： Lee JJ,Srinivasan S,Lim S,Joe M,Im S,Bae SI,Park KR,Han JH,Park SH,Joo BM,Park SJ,Kim MK

更新日期：2014-09-01 00:00:00

abstract：:Trioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with L-isoleucine as a unique starter unit, as well as many...

journal_title：Current microbiology

authors： Yang K,Qi LH,Zhang M,Hou XF,Pan HX,Tang GL,Wang W,Yuan H

更新日期：2015-10-01 00:00:00

abstract：:Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and construct...

journal_title：Current microbiology

authors： Lian C,Zhou Y,Feng F,Chen L,Tang Q,Yao Q,Chen K

更新日期：2014-04-01 00:00:00

abstract：:Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack L-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of L-methioninase (10.78 U/mg protein), rate of methionine uptake (93....

journal_title：Current microbiology

authors： Khalaf SA,El-Sayed AS

更新日期：2009-03-01 00:00:00

abstract：:Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this st...

journal_title：Current microbiology

authors： Gevrekci AÖ

更新日期：2017-06-01 00:00:00

abstract：:A novel thermostable phytase gene was cloned from Aspergillus fumigatus WY-2. It was 1459 bp in size and encoded a polypeptide of 465 amino acids. The gene was expressed in Pichia pastoris GS115 as an extracellular enzyme. The expressed enzyme was purified to homogeneity and biochemically characterized. The purified e...

journal_title：Current microbiology

authors： Wang Y,Gao X,Su Q,Wu W,An L

更新日期：2007-07-01 00:00:00

abstract：:Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. H...

journal_title：Current microbiology

authors： Park SN,Lim YK,Choi MH,Cho E,Bang IS,Kim JM,Ahn SJ,Kook JK

更新日期：2018-01-01 00:00:00

abstract：:To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured...

journal_title：Current microbiology

authors： Yu W,Bing L,Zhenhua L

更新日期：2009-09-01 00:00:00

abstract：:Regulated secretory proteins are sorted via selective co-aggregation in eukaryotes. Cellobiase (C) of the filamentous fungus Termitomyces clypeatus remained co-aggregated with sucrase (S), and only one isoform of each of the enzymes was present in intra- and extracellular extracts. Kinetics of secretion of sucrase inc...

journal_title：Current microbiology

authors： Mukherjee S,Khowala S

更新日期：2002-07-01 00:00:00

abstract：:The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were chara...

journal_title：Current microbiology

authors： Pedersen K,Grisez L,van Houdt R,Tiainen T,Ollevier F,Larsen JL

更新日期：1999-03-01 00:00:00

abstract：:The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...

journal_title：Current microbiology

authors： Cho S,Lee SS

更新日期：2019-01-01 00:00:00

abstract：:In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...

journal_title：Current microbiology

authors： Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang J

更新日期：2013-06-01 00:00:00

abstract：:Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...

journal_title：Current microbiology

authors： Koeduka T,Kajiwara T,Matsui K

更新日期：2007-04-01 00:00:00

abstract：:The taxonomic position of bacterial strain, designated 15J9-4T, recovered from a beach soil sample on Jeju Island, South Korea, was established using a polyphasic approach. Strain 15J9-4T was assigned to phylum Bacteroidetes within the family Cytophagaceae based on 16S rRNA gene similarities. The closest phylogenetic ...

journal_title：Current microbiology

authors： Ten LN,Okiria J,Lee JJ,Lee SY,Park S,Lee DS,Kang IK,Kim MK,Jung HY

更新日期：2018-04-01 00:00:00

abstract：:This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...

journal_title：Current microbiology

authors： Nongkhlaw FM,Joshi SR

更新日期：2016-01-01 00:00:00

abstract：:The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A to...

journal_title：Current microbiology

authors： Al-Talib H,Yean CY,Al-khateeb A,Singh KK,Hasan H,Al-Jashamy K,Ravichandran M

更新日期：2010-07-01 00:00:00

abstract：:The complex gut microbiota plays a key role in host metabolism and health. However, the core microbial communities in the different aged Bactrian camels remain totally unclear. We used high-throughput 16S rRNA gene sequencing to examine the temporal variability of the fecal microbiota in Bactrian camels. At 2 months o...

journal_title：Current microbiology

authors： He J,Hai L,Orgoldol K,Yi L,Ming L,Guo F,Li G,Ji R

更新日期：2019-07-01 00:00:00

abstract：:The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylor...

journal_title：Current microbiology

authors： Simala-Grant JL,Lam E,Keelan M,Taylor DE

更新日期：2004-07-01 00:00:00

abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...

journal_title：Current microbiology

authors： Kim HE,Lee IS,Kim JH,Hahn KW,Park UJ,Han HS,Park KR

更新日期：2003-04-01 00:00:00

abstract：:An autolysis chitinase was purified from the cultural medium of the anaerobic fungus Piromyces communis OTS1 by ammonium sulfate precipitation, affinity chromatography with regenerated chitin, chromato-focusing, gel filtration, and chromato-focusing again. The optimal pH and temperature were 6.0 and 50 degrees C, resp...

journal_title：Current microbiology

authors： Sakurada M,Morgavi DP,Komatani K,Tomita Y,Onodera R

更新日期：1997-07-01 00:00:00

abstract：:The ability of Burkholderia sp. VUN10013 to degrade anthracene in microcosms of two acidic Thai soils was studied. The addition of Burkholderia sp. VUN10013 (initial concentration of 10(5) cells g(-1) dry soil) to autoclaved soil collected from the Plew District, Chanthaburi Province, Thailand, supplemented with anthr...

journal_title：Current microbiology

authors： Somtrakoon K,Suanjit S,Pokethitiyook P,Kruatrachue M,Lee H,Upatham S

更新日期：2008-08-01 00:00:00

abstract：:Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (LsdA). A levanase-encoding gene ( lsdB), starting 51 bp downstream of the lsdA gene, was cloned from strain SRT4. The lsdB gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kDa) containing a putative 36-amino-acid s...

journal_title：Current microbiology

authors： Menéndez C,Hernández L,Selman G,Mendoza MF,Hevia P,Sotolongo M,Arrieta JG

更新日期：2002-07-01 00:00:00

abstract：:Rhodomyrtus tomentosa leaf has been traditionally used to treat many infections. This plant species has been documented to possess a wide spectrum of pharmacological effects. This study aimed to determine the effects of Rhodomyrtus tomentosa leaf extract and its potent purified compound, rhodomyrtone, on Streptococcus...

journal_title：Current microbiology

authors： Mitsuwan W,Wintachai P,Voravuthikunchai SP

更新日期：2020-11-01 00:00:00

abstract：:Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with ...

journal_title：Current microbiology

authors： Akhtar N,Ghauri MA,Akhtar K,Parveen S,Farooq M,Ali A,Schierack P

更新日期：2019-10-01 00:00:00

abstract：:The chromosomal DNA of a variety of legionellae, including clinical, environmental, and plasmid-containing strains, was examined for evidence of Dam-like methylation. It was found that Dam-like methylation was present in all the strains examined regardless of their origin or plasmid content. ...

journal_title：Current microbiology

authors： Lema MW,Brown A

更新日期：1996-02-01 00:00:00

abstract：:We studied the usefulness of flow cytometry for detection of vancomycin resistance in Enterococcus faecalis by direct binding of commercially available fluorescent vancomycin to cells obtained from culture. The cells were stained with Vancomycin@FL, sonicated and additionally stained with propidium iodide (PI). Regard...

journal_title：Current microbiology

authors： Jarzembowski T,Jóźwik A,Wiśniewska K,Witkowski J

更新日期：2010-11-01 00:00:00