Characterization and virulence of hemolysin III from Vibrio vulnificus.


:Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encoding a 214 amino acid polypeptide that showed 48% sequence identity to the hemolysin III of Bacillus cereus. When HlyIII of V. vulnificus was expressed in Escherichia coli, crude extracts exhibited hemolytic activity similar to that of hemolysin III from Bacillus cereus. A hlyIII isogenic mutant was constructed via insertional inactivation and showed an attenuated virulence compared with the wild-type strain when this mutant was administered intraperitoneally in mice.


Curr Microbiol


Current microbiology


Chen YC,Chang MC,Chuang YC,Jeang CL





Has Abstract


2004-09-01 00:00:00












  • Production and characterization of nisin-like peptide produced by a strain of Lactococcus lactis isolated from fermented milk.

    abstract::An isolate of Lactococcus lactis from fermented milk was found to produce a bacteriocin peptide. The isolate could grow in a medium with an initial pH of 11.0, in which it produced the bacteriocin extracellularly at the highest level. The level of the bacteriocin in the medium increased in parallel to the bacterial gr...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Mitra S,Chakrabartty PK,Biswas SR

    更新日期:2005-09-01 00:00:00

  • Pyrosequencing-Based Seasonal Observation of Prokaryotic Diversity in Pneumatophore-Associated Soil of Avicennia marina.

    abstract::Pneumatophores are aerial roots developing from the main roots of mangrove plants away from the gravity. The below ground pneumatophore-associated soil prokaryotic community of Avicennia marina was studied by amplicon pyrosequencing (39,378 reads) during monsoon and summer seasons. Apart from the most dominant phylum ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Sanka Loganathachetti D,Sadaiappan B,Poosakkannu A,Muthuraman S

    更新日期:2016-01-01 00:00:00

  • Pseudomonas pratensis sp. nov., Isolated from Grassland Soil from Inner Mongolia, China.

    abstract::A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...

    journal_title:Current microbiology

    pub_type: 杂志文章,评审


    authors: Zhang M,Shen L,Shi J,Chen L,Luo Y,Sun J,Fan D,Hara S,Bao Z

    更新日期:2021-01-02 00:00:00

  • Analysis of microbial population dynamics in a partial nitrifying SBR at ambient temperature.

    abstract::In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Zeng T,Li D,Zeng H,Zhang Z,Liu L,Zhang X,Zhang J

    更新日期:2013-06-01 00:00:00

  • Bacillus anthracis endospores regulate ornithine decarboxylase and inducible nitric oxide synthase through ERK1/2 and p38 mitogen-activated protein kinases.

    abstract::Interactions between Bacillus anthracis (B. anthracis) and host cells are of particular interest given the implications of anthrax as a biological weapon. Inhaled B. anthracis endospores encounter alveolar macrophages as the first line of defense in the innate immune response. Yet, the consequences of this interaction...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Porasuphatana S,Cao GL,Tsai P,Tavakkoli F,Huwar T,Baillie L,Cross AS,Shapiro P,Rosen GM

    更新日期:2010-12-01 00:00:00

  • Identification of molecular marker and aggressiveness for different groups of Bipolaris sorokiniana isolates causing spot blotch disease in wheat (Triticum aestivum L.).

    abstract::One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the bl...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Jaiswal SK,Sweta,Prasad LC,Sharma S,Kumar S,Prasad R,Pandey SP,Chand R,Joshi AK

    更新日期:2007-08-01 00:00:00

  • Flagellation of Shewanella oneidensis Impacts Bacterial Fitness in Different Environments.

    abstract::Flagella occur on many prokaryotes, which primarily propel cells to move from detrimental to favorable environments. A variety of species-specific flagellation patterns have been identified. Although it is presumed that for each of these flagellated microorganisms, an evolutionarily fixed flagellation pattern is favor...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Yang RS,Chen YT

    更新日期:2020-08-01 00:00:00

  • Mycobacterium tuberculosis expresses ftsE gene through multiple transcripts.

    abstract::Bacterial FtsE gene codes for the ATP-binding protein, FtsE, which in complex with the transmembrane protein, FtsX, participates in diverse cellular processes. Therefore, regulated expression of FtsE and FtsX might be critical to the human pathogen, Mycobacterium tuberculosis, under stress conditions. Although ftsX ge...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Roy S,Vijay S,Arumugam M,Anand D,Mir M,Ajitkumar P

    更新日期:2011-05-01 00:00:00

  • Adhesive property of Bifidobacterium lactis LKM512 and predominant bacteria of intestinal microflora to human intestinal mucin.

    abstract::The adhesive property to the intestinal mucin of Bifidobacterium lactis LKM512, B. longum, B. breve, B. bifidum, B. adolescentis, B. infantis, Bacteroides vulgatus, Bacteroides distasonis, Eubacterium aerofaciens, Clostridium perfringens, Escherichia coli, and Lactobacillus acidophilus were examined. Adhesive rate of ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Matsumoto M,Tani H,Ono H,Ohishi H,Benno Y

    更新日期:2002-03-01 00:00:00

  • Characterization of a novel Cr6+ reducing Pseudomonas sp. with plant growth-promoting potential.

    abstract::The isolate RNP4 obtained from a long-term tannery waste contaminated soil was characterized and presumptively identified as Pseudomonas sp. The strain RNP4 tolerated concentrations up to 450 mg Cr(6+)/L on a Luria-Bartani (LB) agar medium and reduced a substantial amount of Cr(6+) to Cr(3+) in the LB liquid medium. T...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Rajkumar M,Nagendran R,Lee KJ,Lee WH

    更新日期:2005-05-01 00:00:00

  • Correlation between in vitro secretion of virulence-associated proteins of Campylobacter jejuni and colonization of chickens.

    abstract::The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Biswas D,Fernando UM,Reiman CD,Willson PJ,Townsend HG,Potter AA,Allan BJ

    更新日期:2007-03-01 00:00:00

  • Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan.

    abstract::A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0-8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular pola...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Maejima Y,Iino T,Muraguchi Y,Fukuda K,Ohkuma M,Suzuki T,Moriuchi R,Dohra H,Kimbara K,Shintani M

    更新日期:2020-02-01 00:00:00

  • Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae.

    abstract::Microorganisms that colonize plants require a number of hydrolytic enzymes to help degrade the cell wall. The maize endophyte Acremonium zeae was surveyed for production of extracellular enzymes that hydrolyze cellulose and hemicellulose. The most prominent enzyme activity in cell-free culture medium from A. zeae NRRL...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Bischoff KM,Wicklow DT,Jordan DB,de Rezende ST,Liu S,Hughes SR,Rich JO

    更新日期:2009-05-01 00:00:00

  • Characterization of Extracellular Biosurfactants Expressed by a Pseudomonas putida Strain Isolated from the Interior of Healthy Roots from Sida hermaphrodita Grown in a Heavy Metal Contaminated Soil.

    abstract::Pseudomonas putida E41 isolated from root interior of Sida hermaphrodita (grown on a field contaminated with heavy metals) showed high biosurfactant activity. In this paper, we describe data from mass spectrometry and genome analysis, to improve our understanding on the phenotypic properties of the strain. Supernatant...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Bernat P,Nesme J,Paraszkiewicz K,Schloter M,Płaza G

    更新日期:2019-11-01 00:00:00

  • Extracellular alpha-Glucosidase with Dextran-Hydrolyzing Activity from the Thermophilic Fungus, Thermomyces lanuginosus

    abstract::The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Jensen B,Olsen J

    更新日期:1996-09-01 00:00:00

  • A Bacillus thuringiensis isolate possessing a spore-associated filament.

    abstract::We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Rampersad J,Khan A,Ammons D

    更新日期:2003-10-01 00:00:00

  • External and Internal Root Colonization of Maize by TwoPseudomonas Strains: Enumeration by Enzyme-Linked Immunosorbent Assay(ELISA)

    abstract::Maize root colonization by two fluorescentPseudomonas strains M.3.1. and TR335, isolated respectively frommaize and tomato roots, were studied in hydroponic conditions. Each bacteriumwas inoculated separately, and three different colonization areas werestudied: nutrient solution, rhizoplane, and endorhizosphere. The t...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Benizri E,Schoeny A,Picard C,Courtade A,Guckert A

    更新日期:1997-05-01 00:00:00

  • Mycobacterium avium genes upregulated upon infection of Acanthamoeba castellanii demonstrate a common response to the intracellular environment.

    abstract::Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further e...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Tenant R,Bermudez LE

    更新日期:2006-02-01 00:00:00

  • Detection and identification of vegetative insecticidal proteins vip3 genes of Bacillus thuringiensis strains using polymerase chain reaction-high resolution melt analysis.

    abstract::In this study, vegetative insecticidal proteins vip3 genes from Bacillus thuringiensis strains were detected based on polymerase chain reaction-high resolution melt (PCR-HRM) analysis. A pair of primers was designed according to the conservative sequences in 150 bp region of the known vip3 subfamily. The 150 bp region...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Li H,Shu C,He X,Gao J,Liu R,Huang D

    更新日期:2012-05-01 00:00:00

  • Extracellular enzyme activity in a willow sewage treatment system.

    abstract::This paper presents the results of studies on the activity of extra-cellular enzymes in soil-willow vegetation filter soil which is used in the post-treatment of household sewage in an onsite wastewater treatment system located in central Poland. Wastewater is discharged from the detached house by gravity into the ons...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Brzezinska MS,Lalke-Porczyk E,Kalwasińska A

    更新日期:2012-12-01 00:00:00

  • Biogenic amine production by Oenococcus oeni.

    abstract::The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Guerrini S,Mangani S,Granchi L,Vincenzini M

    更新日期:2002-05-01 00:00:00

  • Purification of Endo Polygalacturonases from Sclerotinia sclerotiorum: Multiplicity of the Complex Enzyme System

    abstract::Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Martel MB,Letoublon R,Fevre M

    更新日期:1996-10-01 00:00:00

  • Characterization of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from the halotolerant alga Dunaliella salina and inhibition of its expression by RNAi.

    abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Jia Y,Xue L,Liu H,Li J

    更新日期:2009-05-01 00:00:00

  • iTRAQ-coupled 2D LC-MS/MS analysis of secreted proteome of HBV-replicating HepG2 cells: potential in biomarkers for prognosis of HCC.

    abstract::Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Feng H,Wang M,Chen WN

    更新日期:2010-10-01 00:00:00

  • Antiviral effect of diammonium glycyrrhizinate on cell infection by porcine parvovirus.

    abstract::Porcine parvovirus (PPV) can cause reproductive failure in swine, resulting in economic losses to the industry. Antiviral effects of diammonium glycyrrhizinate (DG) have been reported on several animal viruses; however, to date it has yet to be tested on PPV. In this study, the antiviral activity of DG on swine testis...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Li P,Zou H,Ren Y,Zarlenga DS,Ren X

    更新日期:2014-07-01 00:00:00

  • Variable-number-of-tandem-repeats analysis of genetic diversity in Pasteuria ramosa.

    abstract::Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, a...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Mouton L,Ebert D

    更新日期:2008-05-01 00:00:00

  • Pseudopontivivens aestuariicola gen. nov., sp. nov., a Novel Bacterium of the Class Alphaproteobacteria Isolated from a Tidal Flat.

    abstract::A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Park S,Choi J,Park JM,Yoon JH

    更新日期:2018-11-01 00:00:00

  • Genome Characterization of Oleaginous Aspergillus oryzae BCC7051: A Potential Fungal-Based Platform for Lipid Production.

    abstract::The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Thammarongtham C,Nookaew I,Vorapreeda T,Srisuk T,Land ML,Jeennor S,Laoteng K

    更新日期:2018-01-01 00:00:00

  • Study of lysozyme resistance in Rhodococcus equi.

    abstract::Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Hébert L,Bidaud P,Goux D,Benachour A,Laugier C,Petry S

    更新日期:2014-03-01 00:00:00

  • Mycorrhizal Fungi and Thiobacillus Co-inoculation Improve the Physiological Indices of Lallemantia iberica Under Salinity Stress.

    abstract::Salinity, a serious environmental pressure on crop production, might be counteracted by free-living and symbiotic inoculants entailing positive synergistic effects. Enhancement in nutrient uptake and/or production of antioxidants under the stress condition, can improve plant growth and yield. In this study, inoculatio...

    journal_title:Current microbiology

    pub_type: 杂志文章


    authors: Heydari S,Pirzad A

    更新日期:2020-09-01 00:00:00