Abstract:
:Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encoding a 214 amino acid polypeptide that showed 48% sequence identity to the hemolysin III of Bacillus cereus. When HlyIII of V. vulnificus was expressed in Escherichia coli, crude extracts exhibited hemolytic activity similar to that of hemolysin III from Bacillus cereus. A hlyIII isogenic mutant was constructed via insertional inactivation and showed an attenuated virulence compared with the wild-type strain when this mutant was administered intraperitoneally in mice.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Chen YC,Chang MC,Chuang YC,Jeang CLdoi
10.1007/s00284-004-4288-5keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
175-9issue
3eissn
0343-8651issn
1432-0991journal_volume
49pub_type
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