Abstract:
:Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5 promoter containing completely symmetrical DNA segment that binds more efficiently to the lactose repressor. The beta-galactosidase activity was increased 57-fold when the expression of the re-engineered T5 promoter was induced. The degree of induction was controllable by varying the concentration of inducer isopropyl-beta-D: -thiogalactopyranoside.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Alqublan H,Seleem MN,Boyle SM,Sriranganathan Ndoi
10.1007/s00284-009-9532-6subject
Has Abstractpub_date
2010-04-01 00:00:00pages
242-7issue
4eissn
0343-8651issn
1432-0991journal_volume
60pub_type
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