Tightly regulated expression vectors for Ochrobactrum anthropi.

Abstract:

:Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5 promoter containing completely symmetrical DNA segment that binds more efficiently to the lactose repressor. The beta-galactosidase activity was increased 57-fold when the expression of the re-engineered T5 promoter was induced. The degree of induction was controllable by varying the concentration of inducer isopropyl-beta-D: -thiogalactopyranoside.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Alqublan H,Seleem MN,Boyle SM,Sriranganathan N

doi

10.1007/s00284-009-9532-6

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

242-7

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

60

pub_type

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