Abstract：
:The great increase in the abundance and phylogenetic diversity of Flavobacterium spp. within a few hundred meters downstream of the discharge site of the Westerhöfer Bach, a hardwater rivulet, raised the question whether adjacent soil may serve as a reservoir of bacteria not detected in discharge water. To address this question, denaturing gradient gel electrophoresis (DGGE) analyses of the V3 region of Flavobacterium 16S rRNA genes were performed on DNA from nine soil samples and five rivulet sites. The resulting patterns were tested for the significance of differences between the sampling habitats using the nonparametric analysis of similarities and multidimensional scaling procedures. Even though both habitats were sampled in two consecutive years DGGE patterns of soil and downstream water samples showed significant overlap (R = 0.614). Sequencing of 57 DGGE bands resulted in 30 different sequences, which, on the basis of BLAST analyses, were between 96% and 100% similar to published clone, DGGE, and strain sequences from a wide range of different habitats. Forty-five percent of the highly similar sequences included those of isolates from the Westerhöfer Bach, while the other sequences were more closely related to clones and cultures from other habitats, especially agricultural soil. Based on these results we suggest that the increase in flavobacterial strain diversity and abundance in the rivulet may originate from soil microflora.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Cousin Sdoi
10.1007/s00284-008-9323-5subject
Has Abstractpub_date
2009-05-01 00:00:00pages
409-15issue
5eissn
0343-8651issn
1432-0991journal_volume
58pub_type
杂志文章abstract：:The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expre...
journal_title：Current microbiology
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更新日期：2002-05-01 00:00:00
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更新日期：2016-06-01 00:00:00
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更新日期：2002-11-01 00:00:00
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更新日期：2017-07-01 00:00:00
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journal_title：Current microbiology
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更新日期：1997-10-01 00:00:00
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更新日期：1996-09-01 00:00:00
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更新日期：2020-03-01 00:00:00
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更新日期：2002-07-01 00:00:00
