Abstract:
:Nitric oxide synthase (NOS), the enzyme responsible for the production of endogenous nitric oxide from arginine, has been recently discovered in a number of Gram-positive bacteria. While bacterial NOS has been implicated in mediating nitrosative stress, much remains unknown about the functional role of endogenous nitric oxide in bacteria. Using the known NOS inhibitor aminoguanidine, we examined changes in the protein expression profile using two-dimensional gel electrophoresis. Treatment with aminoguanidine induced several changes in protein expression in Bacillus subtilis. In particular, mreB-like protein (Mbl) was fully down-regulated in the aminoguanidine-treated samples. The expression of Mbl was also examined by reverse transcriptase-polymerase chain reaction and Mbl was found to be fully down-regulated at the transcriptional level as well. Given the role that Mbl plays in the maintenance of cytoskeletal structure, it appears that bacterial NOS may participate in specific biosynthetic pathways with ramifications toward the regulation of antibiotic resistance.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Treece E,Pinkham A,Kim Tdoi
10.1007/s00284-011-0039-6subject
Has Abstractpub_date
2012-02-01 00:00:00pages
112-7issue
2eissn
0343-8651issn
1432-0991journal_volume
64pub_type
杂志文章abstract::Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1176-8
更新日期:2017-02-01 00:00:00
abstract::Previous studies have established that psyllids (Hemiptera, Psylloidea) contain primary endosymbionts, designated as Carsonella ruddii, which cospeciate with the psyllid host. This association appears to be the consequence of a single infection of a psyllid ancestor with a bacterium. Some psyllids may have additional ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010138
更新日期:2000-10-01 00:00:00
abstract::Production of carbon dioxide, as one of the ultimate products of fungal metabolism, can be used to quantify and measure their metabolic rate under different conditions, thus aiding in finding the optimal substrate and environment for cultivation of wood-destroying fungi. This study is focused on species Pleurotus ostr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02033-z
更新日期:2020-09-01 00:00:00
abstract::The ability of non-tuberculous mycobacteria to form biofilms may allow for their increased resistance to currently used biocides in medical and industrial settings. This study examines the biofilm growth of Mycobacterium fortuitum and Mycobacterium marinum, using the MBEC trade mark assay system, and compares the susc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3796-4
更新日期:2003-01-01 00:00:00
abstract::Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0133-8
更新日期:2006-03-01 00:00:00
abstract::Amino acid residues in the active site of quinoline 2-oxidoreductase (Qor) that are deemed important for substrate binding and turnover were replaced by site-directed mutagenesis. The apparent k(cat) values for quinoline were reduced 2.4-, 38-, 40-, and 199-fold in the protein variants QorA259G, QorW331G, QorV373A, an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4452-y
更新日期:2005-04-01 00:00:00
abstract::Proteolytic activity and a subtilisin inhibitor (NSI) were detected in Natrialba magadii cells. The proteolytic activity was due to two different proteases: a approximately 90-kDa metallo protease (NMP) produced during exponential growth and a 246-kDa serine protease (NSP) detected in the stationary phase. Both protea...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3861-z
更新日期:2003-05-01 00:00:00
abstract::Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1225-y
更新日期:2017-05-01 00:00:00
abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900462
更新日期:1999-11-01 00:00:00
abstract::The goal of this study was to evaluate how two new hydrolysates from poultry by-products act on ten lactobacilli growth kinetics when supplemented to the growth medium. These effects were compared with ones induced by two most common commercial hydrolysates, i.e., tryptone and peptone. Growth medium, supplemented with...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0445-z
更新日期:2014-01-01 00:00:00
abstract::Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9585-6
更新日期:2010-08-01 00:00:00
abstract::Cadmium (Cd) and zinc (Zn) are environmental pollutants affecting both soil and water. The toxicity resulting from the exposure of Xanthomonas campestris, a soil bacterium and plant pathogen, to these metals was investigated. Pretreatment of X. campestris with sub-lethal concentrations of Cd induced adaptive protectio...
journal_title:Current microbiology
pub_type: 新闻
doi:10.1007/s00284-003-4025-5
更新日期:2003-09-01 00:00:00
abstract::Lactic-acid-producing bacteria (LABs) are known to have immunomodulating activity. In the current study, various LABs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. Viable but not heat-killed cells of Weissella kimchii strain PL9001, Lactobacillus fermentum strain ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4377-5
更新日期:2005-01-01 00:00:00
abstract::The outer membrane protein of Photobacterium damsela (OMP-PD) and the gene encoding for this porin protein were isolated and characterized. The deduced amino acid sequence of the OMP-PD monomer has 338 amino acids and a calculated molecular weight of 36,951 Da. This sequence includes a 22-amino acid signal peptide at ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4111-8
更新日期:2004-03-01 00:00:00
abstract::The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9977-2
更新日期:2011-09-01 00:00:00
abstract::Variable-number-of-tandem-repeats (VNTR) markers are increasingly being used in population genetic studies of bacteria. They were recently developed for Pasteuria ramosa, an endobacterium that infects Daphnia species. In the present study, we genotyped P. ramosa in 18 infected hosts from the United Kingdom, Belgium, a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9104-1
更新日期:2008-05-01 00:00:00
abstract::This work compared the inhibition effect of the commercially available mouthwash Corsodyl, containing 0.1% chlorhexidine digluconate, and photodynamic inactivation (PDI) employing methylene blue (MB) with irradiation from a red laser on 24-h biofilms formed by Streptococcus mutans strains on hydroxyapatite surfaces. T...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01901-y
更新日期:2020-06-01 00:00:00
abstract::A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910027
更新日期:2000-02-01 00:00:00
abstract::In recent years, Candida albicans infections treatment has become a growing problem because, among others, pathogenic strains are capable to develop resistance to the administered drugs. The elaboration of rapid and accurate method of resistance assessment is an important goal of many studies. They aim to avoid inappr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1039-3
更新日期:2016-08-01 00:00:00
abstract::An endolithic bacterium, strain RSBr-1, was isolated from the inside of a piece of red sandstone from coastal areas of Scotland. RSBr-1 was gram negative, oxidase and catalase positive, and cells were non-motile rods. Sodium was required for growth. The optimum sodium chloride concentration and pH for growth were 4% a...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4006-8
更新日期:2003-11-01 00:00:00
abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02210-0
更新日期:2020-11-01 00:00:00
abstract::DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) is one of the pesticides that are hazardous for the environment and human health. Effective environmental-friendly treatment using co-cultures of fungi and bacteria is needed. In this study, the bacteria Bacillus subtilis at various volumes of 1, 3, 5, 7, and 10 mL (...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1286-y
更新日期:2017-09-01 00:00:00
abstract::A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty ac...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01802-9
更新日期:2020-01-01 00:00:00
abstract::A plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 (B-30488) was isolated from cows' milk. Bacterial colonization and growth responses of different plant species after inoculation with B-30488 were evaluated in a controlled environment and in microplot assays. Survival and colonization of B-30488 in the p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0261-9
更新日期:2006-12-01 00:00:00
abstract::Bradysia difformis is one of the most damaging pests in mushroom production in China. In this study, eight Bacillus thuringiensis strains were analyzed for insecticidal activity in B. difformis. The strain JW-1 showed the highest insecticidal activity against B. difformis larvae, but did not inhibit the mycelial growt...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02018-y
更新日期:2020-10-01 00:00:00
abstract::In this study, the rumen was assessed for its potential to detoxify RDX using molecular microbial ecology as well as analytical chemistry techniques. Results indicated significant loss (P < 0.05) of RDX in <8-h post incubation, and qualitative LC-MS/MS analysis showed evidence for the formation of 1-NO-RDX (M-O + HCOO...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0144-1
更新日期:2012-08-01 00:00:00
abstract::Lactic acid bacteria such as Lactobacillus helveticus, L. delbrueckii subsp. delbrueckii, L. delbrueckii subsp. lactis, L. delbrueckii subsp. bulgaricus, L. acidophilus, and L. casei related taxa which are widely used as starter or probiotic cultures can be identified by amplified ribosomal DNA restriction analysis (A...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840110218
更新日期:2001-04-01 00:00:00
abstract::The connection between soil and microbes plays a critical role in soil health and quality and can be elastic with the application of soil amendments and/or crop rotations. Inappropriate management of soil and application of impermissible levels of fertilizers ruptures the overriding connection between the soil and mic...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02028-w
更新日期:2020-09-01 00:00:00
abstract::The taxonomic position of bacterial strain, designated 15J9-4T, recovered from a beach soil sample on Jeju Island, South Korea, was established using a polyphasic approach. Strain 15J9-4T was assigned to phylum Bacteroidetes within the family Cytophagaceae based on 16S rRNA gene similarities. The closest phylogenetic ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1408-6
更新日期:2018-04-01 00:00:00
abstract::Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (C...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0554-z
更新日期:2007-07-01 00:00:00