Abstract：
:Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify protein expression changes that occur during the post-harvest maturation of Agaricus bisporus fruitbodies at 0, 6, 12, and 48 h after harvest. A total of 5878 unique peptides, representing 1063 proteins, were identified. Quantitative data were obtained from 1012 out of the 1063 proteins. A total of 102, 106, and 160 differentially expressed proteins (>twofolds differences) were identified from samples collected at 6, 12, and 48 h compared to sample from 0 h post harvest, accounting for 10.1, 10.5, and 15.8% of the total proteins identified, respectively. All identified proteins including the differentially expressed proteins among different post-harvest stages were subjected to bioinformatic analysis. Furthermore, seven representative proteins either up or down-regulated at different post-harvest stages were analyzed by real-time PCR. Three out of the seven proteins, the mismatched base pair and cruciform DNA recognition protein, hydrophobin-B and protein transporter, exhibited similar expression patterns as their corresponding proteins. The 260 differentially expressed proteins identified from our study laid a foundation for future studies aiming to understand the post-harvest maturation process of A. bisporus and eventually, might help in the development of breeding program to identify strains with extended shelf life.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Chen M,Liao J,Li H,Cai Z,Guo Z,Wach MP,Wang Zdoi
10.1007/s00284-017-1225-ysubject
Has Abstractpub_date
2017-05-01 00:00:00pages
641-649issue
5eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1225-yjournal_volume
74pub_type
杂志文章abstract：:In laboratory cultures of ice nucleation-active (Ice+) Erwinia herbicola isolates, it has been difficult to achieve high-level expression of ice nuclei, especially nuclei active at temperatures warmer than -5 degrees C (i.e., type 1 ice nuclei). Here we demonstrate that starvation for phosphate and exposure to low tem...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900325
更新日期：1998-06-01 00:00:00
abstract：:The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1498-9
更新日期：2018-08-01 00:00:00
abstract：:Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900107
更新日期：1996-10-01 00:00:00
abstract：:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0085-2
更新日期：2007-09-01 00:00:00
abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1217-y
更新日期：2017-04-01 00:00:00
abstract：:A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-020-02296-6
更新日期：2021-01-02 00:00:00
abstract：:The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900092
更新日期：1996-09-01 00:00:00
abstract：:A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0693-6
更新日期：2015-01-01 00:00:00
abstract：:Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and posse...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0717-2
更新日期：2015-02-01 00:00:00
abstract：:A novel leucine aminopeptidase was purified from a Bacillus thuringiensis israelensis (Bti) culture. The purification stages included heating the concentrated supernatant to 65 degrees C for 90 min, anion-exchange chromatography by DEAE cellulose, and hydrophobic chromatography by phenyl Sepharose. The specific activi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9004-9
更新日期：2007-11-01 00:00:00
abstract：:Brevibacterium halotolerans is currently classified as a member of the Brevibacterium genus, a genus that groups together many bacterial species of similar morphology but diverse biochemical and physiological features. Here we suggest, based on multiple gene sequencing and microbial and biochemical characterization of...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1143-4
更新日期：2017-01-01 00:00:00
abstract：:Pasteurella haemolytica (Ph) is the most important cause of the bovine acute fibrinohemorrhagic pneumonia that occurs in market stressed calves after shipment to feedyards. Recent characterization of neuraminidase production by these organisms has shown that all 16 serotypes produce an immunologically similar form of ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900372
更新日期：1998-10-01 00:00:00
abstract：:PHA is one of the leading commercially important bio-polyesteric compounds piled up as an intracellular lipid-based energy storage compound by numerous microorganisms. An indigenous Gram-positive bacterium isolated from fire ant (Solenopsis invicta) has known to potentially accumulate PHA. Various nutritional elements...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02221-x
更新日期：2020-12-01 00:00:00
abstract：:Bacterial communities communicate, regulate and coordinate their cooperative activities and physiological process by releasing, sensing and responding to small diffusible signal molecules such as acyl homoserine lactones (AHLs), auto-inducing oligo-peptides (AIPs) and autoinducer-2, a process referred to as Quorum sen...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02183-0
更新日期：2020-11-01 00:00:00
abstract：:Streptomyces coelicolor is the soil-dwelling bacterium with a complex life cycle and a strong ability to produce plenty of secondary metabolites which are strictly regulated by a variety of regulators. Amino acid alignment shows that the deduced protein of SCO2140 belongs to the family of Leucine-responsive regulatory...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1050-8
更新日期：2016-08-01 00:00:00
abstract：:Biofouling leads to water quality deterioration and higher maintenance cost for cleaning of membranes. The present study has demonstrated the application of a biomolecule (vanillin) in scrubbing and destabilizing biofilms of drinking water reverse osmosis (RO) membrane module in lab scale reactor set-up. Reverse osmos...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02003-5
更新日期：2020-09-01 00:00:00
abstract：:Bacteria acquire new DNA in a process known as horizontal gene transfer (HGT). To investigate the evolutionary impact of this transfer of DNA, various methods have been developed to detect past HGT events. For example, codon usage-based methods detect the presence of transferred genes by identifying atypical patterns ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0205-5
更新日期：2012-11-01 00:00:00
abstract：:Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01949-w
更新日期：2020-08-01 00:00:00
abstract：:Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to sever...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0178-4
更新日期：2012-10-01 00:00:00
abstract：:A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900127
更新日期：1996-12-01 00:00:00
abstract：:Production of carbon dioxide, as one of the ultimate products of fungal metabolism, can be used to quantify and measure their metabolic rate under different conditions, thus aiding in finding the optimal substrate and environment for cultivation of wood-destroying fungi. This study is focused on species Pleurotus ostr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02033-z
更新日期：2020-09-01 00:00:00
abstract：:The antilisterial efficiency of three bacteriocins from lactic acid bacteria, lactocin 705 (produced by L. casei CRL705, 17000 AU/ml), enterocin CRL35 (produced by E. faecium CRL35, 17000 AU/ml), and nisin (2000 IU/ml), was tested in broth, individually and in combination against Listeria monocytogenes and Listeria in...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010159
更新日期：2000-12-01 00:00:00
abstract：:In the present work, Aspergillus fumigatus is described as a higher producer of hydrolytic enzymes secreted in response to the presence of the Callosobruchus maculatus bruchid pest. This fungus was able to grow over cowpea weevil shells as a unique carbon source, secreting alkaline proteolytic and chitinolytic enzymes...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0192-x
更新日期：2006-06-01 00:00:00
abstract：:The contents of fed-batch composting (FBC) reactors often aggregate after prolonged operation. This process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. We compared the structures of bacterial communities in reactors under aggregate and optimal con...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9107-y
更新日期：2008-05-01 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9333-3
更新日期：2009-05-01 00:00:00
abstract：:This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was exa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9438-3
更新日期：2009-09-01 00:00:00
abstract：:Lactic and butyric acid production by bacterial flocs in a continuous culture obeyed different physiological constraints. The butyric acid rate of production was constant and independent of the growth rate [0.012 +/- 0.001 gBUT/(L.h)], whereas lactic fermentation occurred only beyond a critical growth rate (0.25 +/- 0...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-003-4169-3
更新日期：2004-03-01 00:00:00
abstract：:To understand the mechanism of development of cross-resistance in food pathogen Bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. Pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1090-0
更新日期：2016-10-01 00:00:00
abstract：:Antimicrobial-resistant Klebsiella pneumoniae represent a global public health concern. K. pneumoniae strains isolated during 2010 and 2014-2016 within a single hospital of Molise Region, Central Italy, were analyzed testing antimicrobial susceptibility, clonality by pulsed-field gel electrophoresis (PFGE) and random ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1475-3
更新日期：2018-08-01 00:00:00
abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010186
更新日期：2001-02-01 00:00:00
