Whole-Genome Analysis of an Extensively Drug-Resistance Empedobacter falsenii Strain Reveals Distinct Features and the Presence of a Novel Metallo-ß-Lactamase (EBR-2).

Abstract:

:The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause human infections. E. falsenii Wf282 strain was found to be resistant to many antibiotics, including carbapenems and colistin. Whole-genome shotgun sequencing of the strain was performed, and distinct features were identified. A novel metallo-β-lactamase, named EBR-2, was found, suggesting a potential role of E. falsenii as a reservoir of β-lactamases and other resistance determinants also found in its genome. The EBR-2 protein showed the highest catalytic efficiency for penicillin G as compared to meropenem and ampicillin and was unable to hydrolyze cefepime. The results described in this work broaden the current understanding of the role of β-lactamases in the Flavobacteriaceae family and suggest that E. falsenii Wf282 may be a reservoir of these novel resistance determinants.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Collins C,Almuzara M,Saigo M,Montaña S,Chiem K,Traglia G,Mussi MA,Tolmasky M,Iriarte A,Vay C,Ramirez MS

doi

10.1007/s00284-018-1498-9

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

1084-1089

issue

8

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-018-1498-9

journal_volume

75

pub_type

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