Abstract：
:A broad-host-range vector that expresses a unique artificial RNA in Pseudomonas putida has been developed. This vector was derived from the plasmid pBBR1MCS and incorporates regulatory regions from the Escherichia coli ribosomal operon, rrnB. These include the promoters P1 and P2, and the terminators T1 and T2. The gene for the artificial RNA was derived from Vibrio proteolyticus 5S rRNA. The artificial RNA product accumulates to a level that is 10-20% of the total 5S rRNA in P. putida. The RNA product is not incorporated into ribosomes and has a minimal effect on cell growth rate. In contrast, when wild-type V. proteolyticus 5S rRNA was expressed from the vector, it was incorporated into ribosomes. It is expected that this new vector system will allow artificial RNA expression systems to be readily developed for a large variety of species.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
D'Souza LM,Willson RC,Fox GEdoi
10.1007/s002849910017keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
91-5issue
2eissn
0343-8651issn
1432-0991pii
10.1007/s002849910017journal_volume
40pub_type
杂志文章abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010186
更新日期：2001-02-01 00:00:00
abstract：:Lactic acid bacteria such as Lactobacillus helveticus, L. delbrueckii subsp. delbrueckii, L. delbrueckii subsp. lactis, L. delbrueckii subsp. bulgaricus, L. acidophilus, and L. casei related taxa which are widely used as starter or probiotic cultures can be identified by amplified ribosomal DNA restriction analysis (A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840110218
更新日期：2001-04-01 00:00:00
abstract：:In recent years, Candida albicans infections treatment has become a growing problem because, among others, pathogenic strains are capable to develop resistance to the administered drugs. The elaboration of rapid and accurate method of resistance assessment is an important goal of many studies. They aim to avoid inappr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1039-3
更新日期：2016-08-01 00:00:00
abstract：:The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1350-7
更新日期：2018-01-01 00:00:00
abstract：:Native perennial legume Adesmia bicolor reveals characteristics that are key to securing persistence under grazing. Literature on the diversity and symbiotic effectiveness of indigenous rhizobia-nodulating A. bicolor in central Argentina is limited. The purpose of this study was therefore to determine phenotypic and g...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0260-y
更新日期：2013-02-01 00:00:00
abstract：:The natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, enviro...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0023-1
更新日期：2011-12-01 00:00:00
abstract：:An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02130-z
更新日期：2020-10-01 00:00:00
abstract：:The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900258
更新日期：1997-11-01 00:00:00
abstract：:One hundred and twenty-nine Vibrio anguillarum serogroup O2 strains were compared by slide agglutination and Western blotting for their lipopolysaccharide (LPS) structure. The strains showed six different LPS profiles, four different reaction patterns in Western blotting, and four different kinds of reaction in slide ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900141
更新日期：1997-01-01 00:00:00
abstract：:Temperature and salinity fluctuations are two of the most important factors affecting the growth of polar bacteria. In an attempt to better understand the function of heat-shock proteins (HSPs) in the adaptive mechanisms of the Antarctic psychrotrophic bacterium Psychrobacter sp. G to such conditions, genes Hsp845, Hs...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0409-3
更新日期：2013-11-01 00:00:00
abstract：:The production and its potential use of a novel trihydroxy unsaturated fatty acid, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), were investigated. TOD was formed by Pseudomonas aeruginosa PR3 (NRRL B-18602) in a culture supplied with exogenous ricinoleic acid. The yield of TOD production was always higher in a ric...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010287
更新日期：2001-09-01 00:00:00
abstract：:The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010199
更新日期：2001-03-01 00:00:00
abstract：:A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0584-x
更新日期：2014-09-01 00:00:00
abstract：:We present a novel light-dependent metabolism of an aromatic compound (trans-cinnamate) that is assimilatory rather than dissimilatory. Light-dependent assimilation of trans-cinnamate was observed by both growing and resting cells of Rhodobacter sphaeroides OU5. Trans-cinnamate assimilation could be correlated with si...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0440-8
更新日期：2007-06-01 00:00:00
abstract：:A gene argH, encoding argininosuccinate lyase (ASL), has been cloned from a cosmid library of the filamentous cyanobacterium Nostoc sp. strain PCC 73102. The argH open reading frame encodes a protein comprised of 461 amino acids with a calculated molecular mass of 51,349 Da. Protein sequence comparisons reveal signifi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010298
更新日期：2001-10-01 00:00:00
abstract：:Corynebacterial isolates, obtained by selective and non-selective media from conjunctival swabs, were speciated by using two recently developed systems. All 25 strains from 92 individuals, as well as 12 strains from a previous similar study, belonged to lipophilic species. The significance of this unusual finding is d...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010232
更新日期：2001-05-01 00:00:00
abstract：:Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01739-z
更新日期：2019-12-01 00:00:00
abstract：:Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to sever...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0178-4
更新日期：2012-10-01 00:00:00
abstract：:A Pseudomonas sp. that may be useful in bioremediation projects was isolated from soil. The strain is of potential value because it reduces selenite to elemental red selenium and is unusual in that it was resistant to high concentrations of both selenate and selenite. Exposure of the strain to 50, 100, and 150 mM sele...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9358-2
更新日期：2009-05-01 00:00:00
abstract：:Gut microbiota play a central role in the health of animals. The bacteria that individuals acquire as they age may therefore have a profound effect on their future fitness. Since most birds are capable of flight, they can be widely distributed in and adapted to various ecosystems. Moreover, birds are also challenged b...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02048-6
更新日期：2020-11-01 00:00:00
abstract：:An isolate of Lactococcus lactis from fermented milk was found to produce a bacteriocin peptide. The isolate could grow in a medium with an initial pH of 11.0, in which it produced the bacteriocin extracellularly at the highest level. The level of the bacteriocin in the medium increased in parallel to the bacterial gr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4545-2
更新日期：2005-09-01 00:00:00
abstract：:Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1225-y
更新日期：2017-05-01 00:00:00
abstract：:Lysozyme is an important and widespread component of the innate immune response that constitutes the first line of defense against bacterial pathogens. The bactericidal effect of this enzyme relies on its capacity to hydrolyze the bacterial cell wall and also on a nonenzymatic mechanism involving its cationic antimicr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0483-6
更新日期：2014-03-01 00:00:00
abstract：:The duodenal ulcer promoting gene (dupA), located in the plasticity region of Helicobacter pylori (H. pylori), is predicted to form a type IV secretory system (T4SS) with vir genes around dupA. In the study, we investigated the association between the dupA cluster status and the virulence of H. pylori in a littoral re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0812-z
更新日期：2015-07-01 00:00:00
abstract：:Sheath rot disease of rice caused by Sarocladium oryzae (Sawada) (=Acrocylindrium oryzae, Sawada) has become an important production constraint in all rice-growing countries. Pathogenicity, phytotoxic metabolites, and random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4509-6
更新日期：2005-06-01 00:00:00
abstract：:The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3767-9
更新日期：2002-12-01 00:00:00
abstract：:Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce t...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900268
更新日期：1997-12-01 00:00:00
abstract：:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1217-y
更新日期：2017-04-01 00:00:00
abstract：:Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this st...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1241-y
更新日期：2017-06-01 00:00:00
abstract：:pH affected significantly the growth and the glucose fermentation pattern of Propionibacterium microaerophilum. In neutral conditions (pH 6.5-7.5), growth and glucose fermentation rate (qs) were optimum producing propionate, acetate, CO(2), and formate [which together represented 90% (wt/wt) of the end products], and ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3839-x
更新日期：2003-02-01 00:00:00
