Abstract：
:Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease Control and Prevention (CDC), vaccines and therapeutics are immediately required against this pathogen. In this study, an immunoproteomic approach based on the techniques of 2-dimensional gel electrophoresis (2DE) and immunoblotting combined with mass spectrometry (MS) was used for elucidation of immunogenic components and putative vaccine candidates. Whole-cell soluble protein extract of F. tularensis LVS (Ft LVS) was separated by 2DE, and immunoblots were developed with sera raised in rabbit after immunization with heat-killed Ft LVS. A total of 28 immunoreactive proteins were identified by tandem mass spectrometry. Rabbit immunoproteome of F. tularensis was compared with those previously reported using sera from human patients and in murine model. Out of 28 immunoreactive proteins identified in this study, 12 and 17 overlapping proteins were recognized by human and murine sera, respectively. Nine proteins were found immunogenic in all the three hosts, while eight new immunogenic proteins were found in this study. Identified immunoreactive proteins may find application in design and development of protein subunit vaccine for tularemia.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Gaur R,Alam SI,Kamboj DVdoi
10.1007/s00284-017-1217-ysubject
Has Abstractpub_date
2017-04-01 00:00:00pages
499-507issue
4eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1217-yjournal_volume
74pub_type
杂志文章abstract：:The antilisterial efficiency of three bacteriocins from lactic acid bacteria, lactocin 705 (produced by L. casei CRL705, 17000 AU/ml), enterocin CRL35 (produced by E. faecium CRL35, 17000 AU/ml), and nisin (2000 IU/ml), was tested in broth, individually and in combination against Listeria monocytogenes and Listeria in...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010159
更新日期：2000-12-01 00:00:00
abstract：:Actinomycetes are a wide group of Gram positive prokaryotes, the soil being their most characteristic habitat, where they play important ecological functions. Their immense biotechnological potential as producers of bioactive molecules of great commercial and industrial interest is exemplified by most antibiotics for ...
journal_title：Current microbiology
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doi：10.1007/s00284-020-02030-2
更新日期：2020-09-01 00:00:00
abstract：:The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...
journal_title：Current microbiology
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doi：10.1007/s00284-002-3767-9
更新日期：2002-12-01 00:00:00
abstract：:Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0570-3
更新日期：2014-08-01 00:00:00
abstract：:Proteolytic activity and a subtilisin inhibitor (NSI) were detected in Natrialba magadii cells. The proteolytic activity was due to two different proteases: a approximately 90-kDa metallo protease (NMP) produced during exponential growth and a 246-kDa serine protease (NSP) detected in the stationary phase. Both protea...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3861-z
更新日期：2003-05-01 00:00:00
abstract：:Sheath rot disease of rice caused by Sarocladium oryzae (Sawada) (=Acrocylindrium oryzae, Sawada) has become an important production constraint in all rice-growing countries. Pathogenicity, phytotoxic metabolites, and random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4509-6
更新日期：2005-06-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9692-4
更新日期：2011-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1530-0
更新日期：2018-10-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...
journal_title：Current microbiology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00284-018-1592-z
更新日期：2019-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02028-w
更新日期：2020-09-01 00:00:00
abstract：:A novel actinomycete, designated strain LAM7112T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20-45 °C (optimum: 35 °C), pH 5.0-10.0 (optimum: 7.0) and in the presence of 0-10.0% (optimum: 3.0%) (w/v) NaCl. The ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02192-z
更新日期：2020-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1472-6
更新日期：2018-08-01 00:00:00
abstract：:Regulated secretory proteins are sorted via selective co-aggregation in eukaryotes. Cellobiase (C) of the filamentous fungus Termitomyces clypeatus remained co-aggregated with sucrase (S), and only one isoform of each of the enzymes was present in intra- and extracellular extracts. Kinetics of secretion of sucrase inc...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-7001-y
更新日期：2002-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-01949-w
更新日期：2020-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0116-4
更新日期：2006-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1105-x
更新日期：2016-11-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9038-z
更新日期：2008-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1413-9
更新日期：2018-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9243-4
更新日期：2008-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006828
更新日期：1999-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1090-0
更新日期：2016-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006756
更新日期：1998-03-01 00:00:00
abstract：:Grasslands are suffering from long-term overgrazing because of the population inflation. Furthermore, nitrous oxide (N2O) is a major greenhouse gas that also depletes stratospheric ozone. However, the emission rate of grassland N2O and underlying mechanisms remained unclear under different grazing intensities. We cond...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01668-x
更新日期：2019-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0260-y
更新日期：2013-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9937-x
更新日期：2011-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF02601668
更新日期：1978-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0447-6
更新日期：2006-06-01 00:00:00
abstract：:Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294521
更新日期：1995-01-01 00:00:00
abstract：:Potassium ion pool was studied in glycolyzing Enterococcus hirae, grown at high or low alkaline pH (pH 9.5 and 8.0, respectively). Energy-dependent increase of K+ pool was lower for the wild-type cells, grown at pH 9.5, than that for the cells grown at pH 8.0. It was inhibited by N,N'-dicyclohexylcarbodiimide (DCCD). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900289
更新日期：1998-02-01 00:00:00
