Abstract:
:Diseases triggered by microorganisms can be controlled by vaccines, which need neutralizing antigens. Hence, it is very crucial to identify extremely efficient immunogens for immune prevention. Botulism, a fatal neuroparalytic disease, is caused by botulinum neurotoxins produced by the anaerobic, Gram-positive spore-forming bacteria, Clostridium botulinum. Food-borne botulism and iatrogenic botulism are caused by botulinum toxin. Wound botulism, infant botulism, and adult intestinal botulism are caused by primarily C. botulinum followed by secondary intoxication. To identify protective antigens, whole cell proteome of C. botulinum type B was separated by two-dimensional gel electrophoresis. 2-D gel of whole cell proteins was probed with hyper immune sera of whole cell proteins of C. botulinum types A, E, and F. Six cross immunoreactive proteins were identified. These immunoreactive proteins will be further tested for developing vaccines and serodiagnostic markers against botulism.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Sharma A,Ponmariappan S,Sarita R,Alam SI,Kamboj DV,Shukla Sdoi
10.1007/s00284-017-1413-9subject
Has Abstractpub_date
2018-05-01 00:00:00pages
531-540issue
5eissn
0343-8651issn
1432-0991pii
10.1007/s00284-017-1413-9journal_volume
75pub_type
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