Identification of Cross Reactive Antigens of C. botulinum Types A, B, E & F by Immunoproteomic Approach.

Abstract:

:Diseases triggered by microorganisms can be controlled by vaccines, which need neutralizing antigens. Hence, it is very crucial to identify extremely efficient immunogens for immune prevention. Botulism, a fatal neuroparalytic disease, is caused by botulinum neurotoxins produced by the anaerobic, Gram-positive spore-forming bacteria, Clostridium botulinum. Food-borne botulism and iatrogenic botulism are caused by botulinum toxin. Wound botulism, infant botulism, and adult intestinal botulism are caused by primarily C. botulinum followed by secondary intoxication. To identify protective antigens, whole cell proteome of C. botulinum type B was separated by two-dimensional gel electrophoresis. 2-D gel of whole cell proteins was probed with hyper immune sera of whole cell proteins of C. botulinum types A, E, and F. Six cross immunoreactive proteins were identified. These immunoreactive proteins will be further tested for developing vaccines and serodiagnostic markers against botulism.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Sharma A,Ponmariappan S,Sarita R,Alam SI,Kamboj DV,Shukla S

doi

10.1007/s00284-017-1413-9

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

531-540

issue

5

eissn

0343-8651

issn

1432-0991

pii

10.1007/s00284-017-1413-9

journal_volume

75

pub_type

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