Abstract:
:Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for incidents of severe defoliation in commercially important tree plantations in North America. Culture-based analysis revealed that Pseudomonas spp. along with Bacillus sphaericus and Arthrobacter sp. were the predominant components of the microflora of the internal organs and identified life-stage-specific associations including Photorhabdus temperata with egg and larval samples and a Janthinobacterium sp. with eonymphs. PCR-DGGE confirmed the predominance of Pseudomonas spp. and B. sphaericus in the majority of samples but did not detect Arthrobacter sp., P. temperate, or Janthinobacterium sp. In contrast, DGGE revealed the presence of a Chryseobacterium sp. as the predominant component of the PFW micoflora at all life stages, with the exception of adults. This species had been infrequently cultured, at low levels, from a limited number of samples and the existence of a possible relationship between this bacterium and the PFW had gone unnoticed using the culture-based approach. Our findings highlight the advantages of applying a dual approach to the study of microbe-insect associations and demonstrate that the benefits of one system can be used to overcome some of the limitations of the other.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Zahner V,Lucarotti CJ,McIntosh Ddoi
10.1007/s00284-008-9243-4subject
Has Abstractpub_date
2008-12-01 00:00:00pages
564-9issue
6eissn
0343-8651issn
1432-0991journal_volume
57pub_type
杂志文章abstract::Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01922-7
更新日期:2020-07-01 00:00:00
abstract::Nine domestic wine samples collected from a Japanese winery were examined for the presence of fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3), as well as ochratoxin A (OTA) and ochratoxin B (OTB). Wine samples spiked with 13C-labeled internal standards (13C34-FB1 and 13C20-OTA) were diluted with phospha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02113-0
更新日期:2020-10-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9897-1
更新日期:2011-05-01 00:00:00
abstract::The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9643-0
更新日期:2010-12-01 00:00:00
abstract::Bacillus sp. TYF-LIM-B05, which is isolated from spoilage vinegar, is resistant to high temperature, high concentrated alcohol, acid, and salt, and can produce ethanol from mono-, di-, polysaccharide, and complex biomass as the sole carbon source. Thus, this strain is a potential candidate for consolidated bioprocessi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01833-2
更新日期:2020-03-01 00:00:00
abstract::With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900272
更新日期:1998-01-01 00:00:00
abstract::Pectin is a diverse polysaccharide comprising of polygalacturonic acid, rhamnogalactouronan, and neutral polysaccharides (arabinan and arabinogalactan) as side chains or branches. They are resistant to salivary amylase and gastric juice. In the present study the prebiotic potentials of different pectic derived substra...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01781-x
更新日期:2019-12-01 00:00:00
abstract::A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4-37 °C (optimum: 30 °C) and pH 4-9 (optimum: pH 6), as well as in the presence of 0-6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-sh...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-020-02296-6
更新日期:2021-01-02 00:00:00
abstract::A Gram-positive bacterium, designated strain XY18(T), was isolated from a cured vanilla bean in Hainan province, China. Cells were rod-shaped, endospore producing, and peritrichous flagella. Strain XY18(T) grew at salinities of 0-8 % (w/v) NaCl (optimally 1-4 %), pH 4.0-8.0 (optimally 5.0-7.0 %) and temperature range ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0707-4
更新日期:2015-02-01 00:00:00
abstract::Francisella tularensis, the causative agent of tularemia, has attained the status of one of the high priority agents that could be used in the act of bioterrorism. Currently, there is no licensed vaccine for this highly infectious intracellular pathogen. Being a listed 'Category A' agent of the U.S. Center for Disease...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1217-y
更新日期:2017-04-01 00:00:00
abstract::Viral nervous necrosis infections are causing severe problems on aquaculture industry due to ecological and economic impacts. Their causal agent is nervous necrosis virus or nodavirus, which has been classified into four genotypes. Different genotypes correlate with differences in viral pathogenicity. Therefore, ratio...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0880-0
更新日期:2015-11-01 00:00:00
abstract::A Gram-positive, yellow pigmented actinobacterium, strain S2-48(T) was isolated from water sample collected from the river Brahmaputra, Assam, India and subjected to a polyphasic taxonomic study. Most of the physiological and biochemical properties, major fatty acids (C(15:0) Anteiso, iso C(16:0) and C(17:0) Anteiso),...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9822-z
更新日期:2011-03-01 00:00:00
abstract::The influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from Lactobacillus fermentum CRL 251 was studied. Stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; sc...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900118
更新日期:1996-11-01 00:00:00
abstract::In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9832-x
更新日期:2011-04-01 00:00:00
abstract::The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0490-7
更新日期:2014-03-01 00:00:00
abstract::Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9386-y
更新日期:2009-06-01 00:00:00
abstract::Bacteria use quorum sensing signalling in various functions, e.g. while forming biofilms, and inhibition of this signalling could be one way to control biofilm formation. The aim of this study was to evaluate the production of signalling molecules and its correlation with the biofilm formation capability of bacteria i...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0627-3
更新日期:2014-11-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-007-9052-1
更新日期:2008-02-01 00:00:00
abstract::Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further e...
journal_title:Current microbiology
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更新日期:2006-02-01 00:00:00
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abstract::Genetic engineering of microbes for commercial metabolite production traditionally has sought to alter the levels and/or intrinsic activities of key enzymes in relevant biosynthetic pathway(s). Microorganisms exploit similar strategies for flux control, but also coordinate flux through sets of related pathways by usin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010255
更新日期:2001-07-01 00:00:00
abstract::Gut microbiota play a central role in the health of animals. The bacteria that individuals acquire as they age may therefore have a profound effect on their future fitness. Since most birds are capable of flight, they can be widely distributed in and adapted to various ecosystems. Moreover, birds are also challenged b...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02048-6
更新日期:2020-11-01 00:00:00
abstract::Previous studies have established that psyllids (Hemiptera, Psylloidea) contain primary endosymbionts, designated as Carsonella ruddii, which cospeciate with the psyllid host. This association appears to be the consequence of a single infection of a psyllid ancestor with a bacterium. Some psyllids may have additional ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010138
更新日期:2000-10-01 00:00:00
abstract::The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A to...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9567-8
更新日期:2010-07-01 00:00:00
abstract::The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0021-9
更新日期:2002-05-01 00:00:00
abstract::The newly identified 2019 novel coronavirus (SARS-CoV-2) has become a public health concern globally posing a significant threat to human health and economy and creating an unprecedented crisis in all spheres of the global life. Emergence of new genotypes of SARS-CoV during the last few years has pointed out the limit...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-020-02310-x
更新日期:2021-01-03 00:00:00
abstract::A total, 58 strains of Bacillus thuringiensis were isolated from soils of various regions in Korea. Serological tests showed that B. thuringiensis isolates represented 10 H serotypes, indicating a varied flora of B. thuringiensis. But the H serotypes did not have a significantly uneven distribution, ranging from 1 to ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900363
更新日期:1998-09-01 00:00:00
abstract::NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF00298381
更新日期:1995-10-01 00:00:00
abstract::The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...
journal_title:Current microbiology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00284-018-1592-z
更新日期:2019-01-01 00:00:00
abstract::We report on a novel bacterium, isolated during a screen for environmental isolates of Bacillus thuringiensis, that possesses a novel filamentous structure. Nucleotide sequence from the isolate's 16S rRNA gene places the bacterium unambiguously within the Bacillus thuringiensis/Bacillus cereus group. Phase-contrast an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4103-8
更新日期:2003-10-01 00:00:00