Abstract：
:Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further examination. Upregulation was confirmed in subsequent experiments. A total of 20 clones showed an increase in expression 24 and 48 h after infection. Homologues were identified, and genes were found to encode for a variety of functions, including metabolic pathways, protein transcription and translation, and macromolecule degradation. Eight out of the 20 genes were found to be the same as those upregulated upon human macrophage infection. Five genes were selected to confirm upregulation in M. avium following amoeba infection, using real time PCR. All 5 genes were found to be upregulated at least 2.5-fold in M. avium. These results showed that the GFP promoter library in M. smegmatis is a valid system for studying gene upregulation in M. avium systems, and that many M. avium genes are commonly upregulated following macrophage and amoeba infection.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Tenant R,Bermudez LEdoi
10.1007/s00284-005-0218-4keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
128-33issue
2eissn
0343-8651issn
1432-0991journal_volume
52pub_type
杂志文章abstract：:The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...
journal_title：Current microbiology
pub_type： 杂志文章,已发布勘误
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abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
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abstract：:Together with the worldwide Washoku (traditional Japanese foods and drinks) boom, interest in sake, a traditional Japanese alcoholic drink, is increasing around the world. There are few scientific analyses and studies on the production of sake or the final product itself. We show the diversity of bacterial contaminant...
journal_title：Current microbiology
pub_type： 杂志文章,评审
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abstract：:To present an overview of the existing epidemiological evidence regarding the occurrence of tinea gladiatorum in wrestlers and dermatophyte contamination in wrestling halls, five electronic databases including "PubMed," "Scopus," "Google Scholar," "Science Direct," and "Web of Science" were searched from inception to ...
journal_title：Current microbiology
pub_type： 杂志文章,meta分析
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abstract：:The goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqA mutants in Salmonella typhimurium bacterial cells and UV-C radiations. The exposure changed the fatty acids composition of dam and seqA/dam strai...
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更新日期：2015-10-01 00:00:00
abstract：:Trioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with L-isoleucine as a unique starter unit, as well as many...
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journal_title：Current microbiology
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更新日期：2007-12-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2000-02-01 00:00:00
abstract：:A lytic Pseudomonas aeruginosa phage vB_PaeP_LP14 belonging to the family Podoviridae was isolated from infected mink. The microbiological characterization revealed that LP14 was stable at 40 to 50 °C and stable over a broad range of pH (5 to 12). The latent period was 5 min, and the burst size was 785 pfu/infected ce...
journal_title：Current microbiology
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更新日期：2020-09-01 00:00:00
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更新日期：2007-05-01 00:00:00
abstract：:The ability of non-tuberculous mycobacteria to form biofilms may allow for their increased resistance to currently used biocides in medical and industrial settings. This study examines the biofilm growth of Mycobacterium fortuitum and Mycobacterium marinum, using the MBEC trade mark assay system, and compares the susc...
journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
abstract：:Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0133-8
更新日期：2006-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900185
更新日期：1997-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0445-z
更新日期：2014-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
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doi：10.1007/s00284-020-02034-y
更新日期：2020-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：Current microbiology
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更新日期：2015-06-01 00:00:00
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journal_title：Current microbiology
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更新日期：2001-05-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900413
更新日期：1999-02-01 00:00:00
abstract：:An autolysis chitinase was purified from the cultural medium of the anaerobic fungus Piromyces communis OTS1 by ammonium sulfate precipitation, affinity chromatography with regenerated chitin, chromato-focusing, gel filtration, and chromato-focusing again. The optimal pH and temperature were 6.0 and 50 degrees C, resp...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900210
更新日期：1997-07-01 00:00:00
abstract：:Enterohemorrhagic Escherichia coli (E. coli) O157:H7, a gram-negative bacteria identified as a foodborne pathogen causing severe disease is of great concern worldwide. The pathogenicity of E. coli O157:H7 is due to the presence of some virulence factors and its ability to form biofilm which resist antimicrobial compou...
journal_title：Current microbiology
pub_type： 杂志文章,评审
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更新日期：2017-12-01 00:00:00
abstract：:Insecticidal proteins produced by strains of Bacillus thuringenesis are specific toward target pests. One of the Bt proteins, Cry 1Ac has been used successfully for controlling crop predation by polyphagous pests Helicoverpa armigera. Structurally, Bt proteins consist of three domains; domain I and III are fairly homo...
journal_title：Current microbiology
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doi：10.1007/s00284-010-9749-4
更新日期：2011-02-01 00:00:00
abstract：:Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the gre...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010180
更新日期：2001-01-01 00:00:00
abstract：:The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010111
更新日期：2000-09-01 00:00:00
abstract：:Lactic-acid-producing bacteria (LABs) are known to have immunomodulating activity. In the current study, various LABs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. Viable but not heat-killed cells of Weissella kimchii strain PL9001, Lactobacillus fermentum strain ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4377-5
更新日期：2005-01-01 00:00:00
abstract：:Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. dril...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0565-0
更新日期：2014-08-01 00:00:00
abstract：:We present a novel light-dependent metabolism of an aromatic compound (trans-cinnamate) that is assimilatory rather than dissimilatory. Light-dependent assimilation of trans-cinnamate was observed by both growing and resting cells of Rhodobacter sphaeroides OU5. Trans-cinnamate assimilation could be correlated with si...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0440-8
更新日期：2007-06-01 00:00:00
abstract：:The relationship between 16S rRNA gene sequence-derived phylogeny and the bacterial production of diterpenoids from 18 isolates of marine bacteria belonging to the genus Saprospira was determined. Restriction fragment length polymorphism (RFLP) analysis of the PCR amplified 16S rRNA genes of these isolates indicated f...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4358-8
更新日期：2004-10-01 00:00:00
abstract：:Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein wa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0570-3
更新日期：2014-08-01 00:00:00
