Abstract：
:Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding site, thus competing with ribosomes that would initiate secA translation. Mapping of the geneX-secA mRNA secondary structure has demonstrated that the RNA can adopt two distinct conformations in solution. The first conformation arises from the base-pairing of the secA Shine-Dalgarno (SD) sequence with the geneX terminus. The second conformation, in which the secA SD sequence is no longer paired with the geneX terminus, contains a GC-rich stem upstream of the secA SD sequence. The presence of this GC-rich stem is supported by structure mapping of a mutant RNA containing a deletion in the geneX terminus. The former structure appears to be involved in translational coupling by directly linking the geneX and secA sequences, where geneX translation activates secA translational initiation through the unpairing and unmasking of the secA SD sequence. As indicated by SecA-RNA binding assays, the latter structure is probably involved in SecA binding and translational repression of the secA gene. The stabilizing effect of magnesium ions toward occlusion of the secA SD sequence supports the presence of RNA tertiary structure in this regulatory domain.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Kiser KB,Schmidt MGdoi
10.1007/s002849900413subject
Has Abstractpub_date
1999-02-01 00:00:00pages
113-21issue
2eissn
0343-8651issn
1432-0991journal_volume
38pub_type
杂志文章abstract：:Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3844-0
更新日期：2003-03-01 00:00:00
abstract：:Ansamycins is a group of type I polyketides characterized by the unique starter unit 3-amino-5-hydroxybenzoic acid. This family of secondary metabolites shows diverse biological activities, well-known members of which include rifamycin, geldanamycin, and maytansine. Previously, we isolated an AHBA synthase gene-positi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0798-6
更新日期：2015-06-01 00:00:00
abstract：:Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial Sau3A digestion were cloned into the unique BamHI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC)promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900171
更新日期：1997-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9880-x
更新日期：2011-05-01 00:00:00
abstract：:Pseudomonas aeruginosa is a serious pathogen involved in nosocomial infections. Its pathogenicity is owed to rich production of virulence factors (VIFs) regulated by several complex hierarchical signal systems depending on environmental conditions, medium composition, and the presence of certain active compounds in it...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0441-7
更新日期：2007-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01871-w
更新日期：2020-05-01 00:00:00
abstract：:Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to determine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the or...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0095-4
更新日期：2002-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9762-7
更新日期：2011-02-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-006-0349-2
更新日期：2007-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0623-7
更新日期：2014-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0868-9
更新日期：2015-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-016-1094-9
更新日期：2016-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9158-0
更新日期：2008-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9570-0
更新日期：2010-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9427-6
更新日期：2009-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0243-z
更新日期：2013-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0435-1
更新日期：2014-01-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010128
更新日期：2000-10-01 00:00:00
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journal_title：Current microbiology
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更新日期：2011-04-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/pl00006793
更新日期：1999-04-01 00:00:00
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更新日期：2015-01-01 00:00:00
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journal_title：Current microbiology
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更新日期：2014-06-01 00:00:00
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journal_title：Current microbiology
pub_type： 新闻
doi：10.1007/s00284-003-4025-5
更新日期：2003-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2000-12-01 00:00:00
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journal_title：Current microbiology
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doi：10.1007/s00284-017-1400-1
更新日期：2018-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-001-0039-7
更新日期：2002-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900240
更新日期：1997-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010287
更新日期：2001-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4288-5
更新日期：2004-09-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02221-x
更新日期：2020-12-01 00:00:00
