A novel antifungal Pseudomonas fluorescens isolated from potato soils in Greenland.

Abstract:

:A rhizobacterium with high antifungal activity was isolated from a potato field at Inneruulalik, South Greenland. Phylogenetic analysis based on multi locus sequence typing showed that the bacterium was affiliated with strains of Pseudomonas fluorescens. The bacterium, denoted as Pseudomonas fluorescens In5, inhibited in vitro a broad range of phytopathogenic fungi, and the antifungal activity increased with decreasing temperature. Microcosm experiments demonstrated that P. fluorescens In5 protected tomato seedlings from Rhizoctonia solani. Transposon mutagenesis showed that the major cause for the antifungal activity of P. fluorescens In5 was a novel non-ribosomal peptide synthase (NRPS) gene. In addition, transposon mutagenesis showed that P. fluorescens In5 also contained a putative quinoprotein glucose dehydrogenase gene, which was involved in growth inhibition of phytopathogenic fungi. Although P. fluorescens In5 contained the capacity to synthesize hydrogen cyanide, β-1,3-glucanase, protease, and chitinase, these did not seem to play a role in the in vitro and microcosm antifungal assays.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Michelsen CF,Stougaard P

doi

10.1007/s00284-010-9846-4

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

1185-92

issue

4

eissn

0343-8651

issn

1432-0991

journal_volume

62

pub_type

杂志文章
  • Detection and Determination of Fumonisins B1, B2, and B3 Contaminating Japanese Domestic Wine by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS).

    abstract::Nine domestic wine samples collected from a Japanese winery were examined for the presence of fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3), as well as ochratoxin A (OTA) and ochratoxin B (OTB). Wine samples spiked with 13C-labeled internal standards (13C34-FB1 and 13C20-OTA) were diluted with phospha...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02113-0

    authors: Nakagawa H,Hashimoto R,Matsuo Y,Sago Y,Yokoyama K,Takahashi H

    更新日期:2020-10-01 00:00:00

  • Identification and partial sequence of the BchA gene of Rhodospirillum rubrum.

    abstract::The DNA sequence was determined for a region upstream of the puf operon of Rhodospirillum rubrum. A partial ORF was identified. The DNA sequence and the inferred amino acid sequences were aligned with those of bchA of Rhodobacter capsulatus and other phototrophic bacteria. Based on this alignment and genetic evidence,...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01570863

    authors: Lee IY,Collins ML

    更新日期:1993-08-01 00:00:00

  • Biofilm Formation by Avian Pathogenic Escherichia coli is Not Related to In Vivo Pathogenicity.

    abstract::Avian pathogenic Escherichia coli (APEC) is one of the pathogens that most concerns the poultry industry worldwide due to the economic losses it can cause. APEC persistence and survival, both in the environment and in the host, may be a consequence of biofilm-producing capabilities. The aim of this study was to evalua...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1608-8

    authors: Rodrigues SV,Laviniki V,Borges KA,Furian TQ,Moraes HLS,Nascimento VP,Salle CTP

    更新日期:2019-02-01 00:00:00

  • Pseudopontivivens aestuariicola gen. nov., sp. nov., a Novel Bacterium of the Class Alphaproteobacteria Isolated from a Tidal Flat.

    abstract::A Gram-stain-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, OITF-57T, which was isolated from a tidal flat sediment in South Korea, was characterized taxonomically. Strain OITF-57T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain OITF-57T exhibited the high...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1553-6

    authors: Park S,Choi J,Park JM,Yoon JH

    更新日期:2018-11-01 00:00:00

  • Nucleotide sequence of the gene coding for non-proteolytic Clostridium botulinum type B neurotoxin: comparison with other clostridial neurotoxins.

    abstract::The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primer...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01569055

    authors: Hutson RA,Collins MD,East AK,Thompson DE

    更新日期:1994-02-01 00:00:00

  • Development of a Semi-nested PCR-Based Method for Specific and Rapid Detection of Alternaria solani Causing Potato Early Blight in Soil.

    abstract::Early blight, caused by Alternaria solani, is one of the most devastating diseases of potato that causes severe yield loss worldwide. The infected potato debris existed in the soil serve as the initial infection sources for the next growing potato. Current identification of A. solani in soil relies primarily on cultur...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1284-0

    authors: Gu Q,Yang ZH,Zhao DM,Zhang D,Wang Q,Ma LS,Zhu JH

    更新日期:2017-09-01 00:00:00

  • Cloning, expression, and enzyme characterization of an acid heat-stable phytase from Aspergillus fumigatus WY-2.

    abstract::A novel thermostable phytase gene was cloned from Aspergillus fumigatus WY-2. It was 1459 bp in size and encoded a polypeptide of 465 amino acids. The gene was expressed in Pichia pastoris GS115 as an extracellular enzyme. The expressed enzyme was purified to homogeneity and biochemically characterized. The purified e...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0613-5

    authors: Wang Y,Gao X,Su Q,Wu W,An L

    更新日期:2007-07-01 00:00:00

  • Presence of qacEΔ1 gene and susceptibility to a hospital biocide in clinical isolates of Pseudomonas aeruginosa resistant to antibiotics.

    abstract::Biocides play an important role in healthcare-associated infection control by either minimizing or preventing microorganism dissemination. This study evaluated the susceptibility of Pseudomonas aeruginosa clinical isolates to a quaternary ammonium (QAC) disinfectant and antibiotics, and verified the presence of qacEΔ1...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9934-0

    authors: Romão C,Miranda CA,Silva J,Mandetta Clementino M,de Filippis I,Asensi M

    更新日期:2011-07-01 00:00:00

  • Bacteria associated with butterfly stain of Chilean tepa.

    abstract::Butterfly stain, common in Chilean tepa (Laureliopsis philippiana [Looser] Schodde) trees, appears in cross-section of the stem as a series of partially overlapping orange-brown arcs that in early stages resembles a butterfly. Bacterial isolates from stained tepa wood samples cultured under aerobic and anaerobic condi...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849910016

    authors: McLemore JB,Tainter FH,McElreath SD

    更新日期:2000-02-01 00:00:00

  • Transformation in heterokaryons of Neurospora crassa is nuclear rather than cellular phenomenon.

    abstract::In Neurospora crassa, multinucleate macroconidia are used for genetic transformation. The barrier for such a transformation can be either at the cell membrane level or at the nuclear membrane level. For assessment of these possibilities, a forced heterokaryon (containing two genetically marked nuclei and auxotrophic f...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-001-0004-x

    authors: Dev K,Maheshwari R

    更新日期:2002-05-01 00:00:00

  • Rhesus monkey TRIM5α has distinct HIV-1 restriction activity among different mammalian cell lines.

    abstract::Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-0009-z

    authors: Gong J,Shen XH,Qiu H,Chen C,Yang RG

    更新日期:2011-12-01 00:00:00

  • Identification and Analysis of a Novel Gene Cluster Involves in Fe2+ Oxidation in Acidithiobacillus ferrooxidans ATCC 23270, a Typical Biomining Acidophile.

    abstract::Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for the dissolution of sulfide minerals. However, molecular mechanisms of their iron oxidation still remain elusive. A nove...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1453-9

    authors: Ai C,Liang Y,Miao B,Chen M,Zeng W,Qiu G

    更新日期:2018-07-01 00:00:00

  • The metabolism of aluminum citrate and biosynthesis of oxalic acid in Pseudomonas fluorescens.

    abstract::(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3944-x

    authors: Appanna VD,Hamel RD,Lévasseur R

    更新日期:2003-07-01 00:00:00

  • Identification of a novel virulence-related gene in Streptococcus suis type 2 strains.

    abstract::The trag is a novel infection-related factor identified using in vivo-induced antigen technology (IVIAT) from SS2 expression libraries with swine convalescent sera. PCR-based studies of various S. suis strains collected from different sources revealed that the trag gene was present in all the virulent SS2 strains, but...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9643-0

    authors: Zhang H,Fan H,Lu C

    更新日期:2010-12-01 00:00:00

  • Tightly regulated expression vectors for Ochrobactrum anthropi.

    abstract::Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9532-6

    authors: Alqublan H,Seleem MN,Boyle SM,Sriranganathan N

    更新日期:2010-04-01 00:00:00

  • The tracing of mycobacteria in drinking water supply systems by culture, conventional, and real time PCRs.

    abstract::Mycobacteria are widely present in diverse aquatic habitats, where they can survive for months or years while some species can even proliferate. The resistance of different mycobacterial species to disinfection methods like chlorination or ozonation could result in their presence in the final tap water of consumers. I...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-013-0427-1

    authors: Klanicova B,Seda J,Slana I,Slany M,Pavlik I

    更新日期:2013-12-01 00:00:00

  • Complete Genome Sequences of Leclercia sp. W6 and W17 Isolated from a Gastric Cancer Patient.

    abstract::Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-020-02075-3

    authors: Xu YY,Huang CJ,Xu L,Jiang XW,Xu XW,Xu XW

    更新日期:2020-10-01 00:00:00

  • Differential roles of the two-component peptides of lactocin 705 in antimicrobial activity.

    abstract::Lactobacillus casei CRL705 produces a class IIb bacteriocin, lactocin 705, which relies on the complementary action of two components, Lac705alpha and Lac705beta. These peptides exert a bactericidal effect on the indicator strain Lactobacillus plantarum CRL691, with an optimal Lac705alpha/Lac705beta peptide ratio of 1...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3844-0

    authors: Cuozzo SA,Castellano P,Sesma FJ,Vignolo GM,Raya RR

    更新日期:2003-03-01 00:00:00

  • Carbon catabolite control is important for Listeria monocytogenes biofilm formation in response to nutrient availability.

    abstract::The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. The biofilm formation is strongly influenced by the availability of nutrients and environmental conditions, and particularly enhanced in poor minimal essen...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-012-0125-4

    authors: Zhou Q,Feng X,Zhang Q,Feng F,Yin X,Shang J,Qu H,Luo Q

    更新日期:2012-07-01 00:00:00

  • Clone Q379 immunoscreened from a Chinese HCV cDNA lambda gt11 library.

    abstract::A Chinese HCV cDNA clone, designated as Q379, 379 nucleotides in length, was selected by recombinant immunoscreening from a random-primed Chinese HCV lambda gt11 library. DNA sequencing demonstrated that Q379 situated from positions 7314 to 7695 at NS5 region corresponded with the American prototype HCV nucleotide seq...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01571058

    authors: Qi Z,Arima T

    更新日期:1994-03-01 00:00:00

  • Evaluation of the Pseudalert/Quanti-Tray MPN Test for the Rapid Enumeration of Pseudomonas aeruginosa in Swimming Pool and Spa Pool Waters.

    abstract::This study assessed the performance of a new most probable number test (Pseudalert/Quanti-Tray) for the enumeration of Pseudomonas aeruginosa from swimming pool and spa pool waters by comparing it to the international and national membrane filtration-based culture methods for P. aeruginosa: ISO 16266:2006 and UK The M...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0905-8

    authors: Sartory DP,Brewer M,Beswick A,Steggles D

    更新日期:2015-12-01 00:00:00

  • Long-term survival of Bacillus spores in alcohol and identification of 90% ethanol as relatively more spori/bactericidal.

    abstract::This study was taken up with a view to generate basic information on spore hardiness to ethanol in various Bacillus species and related genera, and to assess the effectiveness of different levels of ethanol as a bacterial disinfectant. Predominantly spore-bearing cultures of five Bacillus spp. (B. pumilus, B. subtilis...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-0040-0

    authors: Thomas P

    更新日期:2012-02-01 00:00:00

  • Cloning and characterization of two novel crystal protein genes, cry54Aa1 and cry30Fa1, from Bacillus thuringiensis strain BtMC28.

    abstract::Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9386-y

    authors: Tan F,Zhu J,Tang J,Tang X,Wang S,Zheng A,Li P

    更新日期:2009-06-01 00:00:00

  • Biofilm formation by Salmonella enterica serovar 1,4,[5],12:i:- Portuguese isolates: a phenotypic, genotypic, and socio-geographic analysis.

    abstract::Biofilm-forming ability is well established as an important virulence factor. However, there are no studies available regarding biofilm formation of Salmonella Typhimurium 1,4,[5],12:i:-, the new pandemic serovar in Europe. To address this problem, biofilm expression by Salmonella 1,4,[5],12:i:- was evaluated using 13...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-014-0523-x

    authors: Seixas R,Machado J,Bernardo F,Vilela C,Oliveira M

    更新日期:2014-05-01 00:00:00

  • Ruminal Microbial Populations and Fermentation Characteristics in Beef Cattle Grazing Tropical Forage in Dry Season and Supplemented with Different Protein Levels.

    abstract::We tested the hypothesis that supplementation with protein improves fermentation parameters without damaging the rumen microbial populations of beef cattle grazing Urochloa brizantha cv. Marandu during the dry season. Four rumen-cannulated Nellore bulls (571 ± 31 kg of body weight) were used in a 4 × 4 Latin square de...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01631-w

    authors: da Silva-Marques RP,Zervoudakis JT,Nakazato L,Hatamoto-Zervoudakis LK,da Silva Cabral L,do Nascimento Matos NB,da Silva MIL,Feliciano AL

    更新日期:2019-03-01 00:00:00

  • Enhanced Production of Recombinant Alcohol Dehydrogenase Using the Genetically Engineered Escherichia coli Strain that Heterologously Expresses Carrot Heat Shock Protein 70.

    abstract::Escherichia coli (E. coli) has been widely used as a host organism for producing recombinant proteins such as biocatalysts, antibody fragments, and therapeutic hormones. To enhance recombinant protein production, many E. coli strains have been genetically engineered on practical purposes. In this study, we developed t...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01730-8

    authors: Kim M,Jang B,Ahn YJ

    更新日期:2019-11-01 00:00:00

  • Physical mapping of the Bacillus thuringiensis subsp. kurstaki and alesti chromosomes.

    abstract::Two strains of the well-known insect pathogen and biopesticide, Bacillus thuringiensis (Bt), belonging to subspecies alesti (strain Bt5) and kurstaki (strain Bt213), were chosen for genetic characterization. The two strains belong to different serotypes and are currently classified into different subspecies, although ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-001-0055-z

    authors: Lövgren A,Carlson CR,Kang D,Eskils K,Kolstø AB

    更新日期:2002-02-01 00:00:00

  • Isolation and identification of a novel strain of Pseudomonas chlororaphis capable of transforming isoeugenol to vanillin.

    abstract::Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0627-z

    authors: Kasana RC,Sharma UK,Sharma N,Sinha AK

    更新日期:2007-06-01 00:00:00

  • Single-Nucleotide Polymorphisms in the Whole-Genome Sequence Data of Shiga Toxin-Producing Escherichia coli O157:H7/H- Strains by Cultivation.

    abstract::Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleoti...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1208-z

    authors: Yokoyama E,Hirai S,Ishige T,Murakami S

    更新日期:2017-04-01 00:00:00

  • Diversity of bacteria and fungi in aerosols during screening in a green waste composting plant.

    abstract::This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was exa...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9438-3

    authors: Bru-Adan V,Wéry N,Moletta-Denat M,Boiron P,Delgènes JP,Godon JJ

    更新日期:2009-09-01 00:00:00