Abstract：
:A novel thermostable phytase gene was cloned from Aspergillus fumigatus WY-2. It was 1459 bp in size and encoded a polypeptide of 465 amino acids. The gene was expressed in Pichia pastoris GS115 as an extracellular enzyme. The expressed enzyme was purified to homogeneity and biochemically characterized. The purified enzyme had a specific activity of 51 U/mg with an approximate molecular mass of 88 kDa. The optimum pH and temperature for activity were pH 5.5 and 55 degrees C, respectively. After incubation at 90 degrees C for 15 min, it still remained at 43.7% of the initial activity. The enzyme showed higher affinity for sodium phytate than other phosphate conjugates, and the K(m) and K(cat) for sodium phytate were 114 microM: and 102 s(-1), respectively. Incubated with pepsin at 37 degrees C for 2 h at the ratio (pepsin/phytase, wt/wt) of 0.1, it still retained 90.1% residual activity. These exceptional properties give the newly cloned enzyme good potential in animal feed applications.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Wang Y,Gao X,Su Q,Wu W,An Ldoi
10.1007/s00284-006-0613-5subject
Has Abstractpub_date
2007-07-01 00:00:00pages
65-70issue
1eissn
0343-8651issn
1432-0991journal_volume
55pub_type
杂志文章abstract：:Burkholderia cepacia is an opportunistic pathogen that causes serious pulmonary infections in cystic fibrosis patients. Although several potential virulence factors-a protease, lipase, and two phospholipases C (one hemolytic and one nonhemolytic)-have been identified, only two, the protease and the lipase, have been d...
journal_title：Current microbiology
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更新日期：2020-10-01 00:00:00
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journal_title：Current microbiology
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更新日期：2010-07-01 00:00:00
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更新日期：2002-10-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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更新日期：2006-11-01 00:00:00
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journal_title：Current microbiology
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更新日期：2002-06-01 00:00:00
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pub_type： 杂志文章,已发布勘误
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更新日期：2019-01-01 00:00:00
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更新日期：2002-11-01 00:00:00
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更新日期：2005-01-01 00:00:00
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journal_title：Current microbiology
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journal_title：Current microbiology
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更新日期：2000-04-01 00:00:00
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更新日期：2002-12-01 00:00:00
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更新日期：2006-06-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2020-04-01 00:00:00
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更新日期：2001-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章,评审
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journal_title：Current microbiology
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更新日期：2011-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 信件
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更新日期：2017-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0218-4
更新日期：2006-02-01 00:00:00
