Molecular cloning and characterization of nodD genes from Rhizobium sp. SIN-1, a nitrogen-fixing symbiont of Sesbania and other tropical legumes.

abstract：:In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasm...

journal_title：Current microbiology

authors： Liu Z,Xie Y,Zhang X,Hu X,Li Y,Ding X,Xia L,Hu S

更新日期：2016-04-01 00:00:00

abstract：:Yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental pH. Previous results have indicated that secretion of the alkaline protease is under control of the pH signaling Pal/Rim pathway originally described in Aspergillus nidulans. Several Y. lipol...

journal_title：Current microbiology

authors： González-López CI,Ortiz-Castellanos L,Ruiz-Herrera J

更新日期：2006-07-01 00:00:00

abstract：:Lysobacter enzymogenes OH11 produces heat-stable antifungal factor (HSAF) and lytic enzymes possessing antifungal activity. This study bio-prospected for other potential antifungal factors besides those above. The cells and extracellular metabolites of L. enzymogenes OH11 and the mutants ΔchiA, ΔchiB, ΔchiC, Δclp, Δpk...

journal_title：Current microbiology

authors： Odhiambo BO,Xu G,Qian G,Liu F

更新日期：2017-04-01 00:00:00

abstract：:PCR has been widely used to identify cry-type genes, to determine their distribution, to detect new such genes and to predict insecticidal activities. We describe here a molecular approach to analyze the genetic diversity of B. thuringiensis cry-like genes based on denaturing gradient gel electrophoresis (DGGE). This ...

journal_title：Current microbiology

authors： Vidal-Domínguez ME,Perez-Cenci M,Salerno GL,Berón CM

更新日期：2011-03-01 00:00:00

abstract：:A total, 58 strains of Bacillus thuringiensis were isolated from soils of various regions in Korea. Serological tests showed that B. thuringiensis isolates represented 10 H serotypes, indicating a varied flora of B. thuringiensis. But the H serotypes did not have a significantly uneven distribution, ranging from 1 to ...

journal_title：Current microbiology

authors： Kim HS,Lee DW,Woo SD,Yu YM,Kang SK

更新日期：1998-09-01 00:00:00

abstract：:A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest ...

journal_title：Current microbiology

authors： Lee JJ,Srinivasan S,Lim S,Joe M,Im S,Bae SI,Park KR,Han JH,Park SH,Joo BM,Park SJ,Kim MK

更新日期：2014-09-01 00:00:00

abstract：:One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the bl...

journal_title：Current microbiology

authors： Jaiswal SK,Sweta,Prasad LC,Sharma S,Kumar S,Prasad R,Pandey SP,Chand R,Joshi AK

更新日期：2007-08-01 00:00:00

abstract：:Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...

journal_title：Current microbiology

authors： Radice F,Orlandi V,Massa V,Cavalca L,Demarta A,Wood TK,Barbieri P

更新日期：2006-05-01 00:00:00

abstract：:To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and...

journal_title：Current microbiology

authors： Liu H,Bao X

更新日期：2009-03-01 00:00:00

abstract：:A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S ...

journal_title：Current microbiology

authors： Stackebrandt E,Frühling A,Cousin S,Brambilla E,Lünsdorf H,Verbarg S

更新日期：2008-04-01 00:00:00

abstract：:A Gram-positive bacterium, designated strain XY18(T), was isolated from a cured vanilla bean in Hainan province, China. Cells were rod-shaped, endospore producing, and peritrichous flagella. Strain XY18(T) grew at salinities of 0-8 % (w/v) NaCl (optimally 1-4 %), pH 4.0-8.0 (optimally 5.0-7.0 %) and temperature range ...

journal_title：Current microbiology

authors： Chen YG,Gu FL,Li JH,Xu F,He SZ,Fang YM

更新日期：2015-02-01 00:00:00

abstract：:Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (C...

journal_title：Current microbiology

authors： Sugiarto H,Yu PL

更新日期：2007-07-01 00:00:00

abstract：:Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...

journal_title：Current microbiology

authors： Gírio FM,Roseiro JC,Silva AI

更新日期：1998-06-01 00:00:00

abstract：:Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A...

journal_title：Current microbiology

authors： Iwaki H,Nakai E,Nakamura S,Hasegawa Y

更新日期：2008-08-01 00:00:00

abstract：:A 4.5-kilobase DNA fragment from Buchnera aphidicola, the endosymbiont of the aphid Schizaphis graminum, was cloned and sequenced. On the basis of homology to Escherichia coli, the following genes were found in the order listed: aroH-thrS-infC-rpmI-rplT. AroH corresponds to the E. coli tryptophan-inhibited 3-deoxy-D-a...

journal_title：Current microbiology

authors： Kolibachuk D,Rouhbakhsh D,Baumann P

更新日期：1995-05-01 00:00:00

abstract：:The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using ...

journal_title：Current microbiology

authors： Goto K,Ohashi H,Takakura A,Itoh T

更新日期：2000-09-01 00:00:00

abstract：:A lytic Pseudomonas aeruginosa phage vB_PaeP_LP14 belonging to the family Podoviridae was isolated from infected mink. The microbiological characterization revealed that LP14 was stable at 40 to 50 °C and stable over a broad range of pH (5 to 12). The latent period was 5 min, and the burst size was 785 pfu/infected ce...

journal_title：Current microbiology

authors： Shi X,Zhao F,Sun H,Yu X,Zhang C,Liu W,Pan Q,Ren H

更新日期：2020-09-01 00:00:00

abstract：:Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and ex...

journal_title：Current microbiology

authors： Nerey Md Mdel C,Pichuantes SE,Saavedra CP,Araya MA,Tantaleán JC,Vásquez CC

更新日期：2002-09-01 00:00:00

abstract：:Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. ...

journal_title：Current microbiology

authors： Dong Q,Xin Y,Wang L,Meng X,Yu X,Lu L,Xuan S

更新日期：2017-02-01 00:00:00

abstract：:Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties ...

journal_title：Current microbiology

authors： Bozkurt-Guzel C,Inci G,Oyardi O,Savage PB

更新日期：2020-08-01 00:00:00

abstract：:Nitrate assimilation-defective mutants SP7, SP9, and SP17 of the cyanobacterium Anabaena sp. PCC 7120 were isolated by use of transposon mutagenesis and screened on medium containing chlorate. SP7 and SP17 represented nitrate reductase-defective nature, while mutant SP9 appeared to be a regulatory mutant exhibiting pl...

journal_title：Current microbiology

authors： Rai AK,Tiwari SP

更新日期：1999-11-01 00:00:00

abstract：:The Gram-stain-negative, rod-shaped, aerobic strain, designated YC1039T, was isolated from a seamount northern Mariana Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC1039T was related to the genus Mesoflavibacter and had highest 16S rRNA gene sequence...

journal_title：Current microbiology

authors： Wang DY,Wang Q,Liu J,Zhang DC

更新日期：2018-09-01 00:00:00

abstract：:An aerobic, gram-reaction-negative, rod-shaped bacterium, designated strain Z6(T), was isolated from sediment collected at Mohe Basin, China. And its taxonomic position was investigated by applying a polyphasic approach. Growth occurs at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 7.0) and in the presence o...

journal_title：Current microbiology

authors： Han L,Teng YW,Feng QQ,Zhang RG,Zhao XM,Lv J,Xie B

更新日期：2016-09-01 00:00:00

abstract：:This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac ...

journal_title：Current microbiology

authors： Beard CE,Court L,Mourant RG,James B,Van Rie J,Masson L,Akhurst RJ

更新日期：2008-09-01 00:00:00

abstract：:Root colonization and diversity of arbuscular mycorrhizal fungi (AMF) were analyzed in plants growing in fly ash pond. Eight species could be separated morphologically, while phylogenetic analyses after PCR amplification of the ITS region followed by RFLP and sequencing revealed seven different AM fungal sequence type...

journal_title：Current microbiology

authors： Giridhar Babu A,Sudhakara Reddy M

更新日期：2011-09-01 00:00:00

abstract：:The complex gut microbiota plays a key role in host metabolism and health. However, the core microbial communities in the different aged Bactrian camels remain totally unclear. We used high-throughput 16S rRNA gene sequencing to examine the temporal variability of the fecal microbiota in Bactrian camels. At 2 months o...

journal_title：Current microbiology

authors： He J,Hai L,Orgoldol K,Yi L,Ming L,Guo F,Li G,Ji R

更新日期：2019-07-01 00:00:00

abstract：:The production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when Mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/L of Mg2+ at 23 degrees C for 5 days. Cultivation conditions were studied, and the results showed that (i) a suitabl...

journal_title：Current microbiology

authors： Xian M,Kang Y,Yan J,Liu J,Bi Y,Zhen K

更新日期：2002-02-01 00:00:00

abstract：:Plasmids play a central role in engineering recombinant bacteria because they are the primary vehicles used to manipulate targeted sequences. In some cases, bacteria of interest are poorly provided with suitable tools for these molecular or genetic manipulations. In this context, we constructed from two shuttle clonin...

journal_title：Current microbiology

authors： Benachour A,Auffray Y,Hartke A

更新日期：2007-05-01 00:00:00

abstract：:Minimal inhibitory concentrations (MICs) of five antibiotics namely amikacin, gentamicin, tetracycline, ciprofloxacin, and nitrofurantoin for pork isolates of Yersinia enterocolitica increased two- to eightfold after bacteria were grown in the presence of 5 mm arsenite. For Y. enterocolitica isolates obtained from was...

journal_title：Current microbiology

authors： Virdi JS,Sinha I,Rajendran P,Singh I

更新日期：2001-08-01 00:00:00

abstract：:Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...

journal_title：Current microbiology

authors： Williams PD,Eichstadt SL,Kokjohn TA,Martin EL

更新日期：2007-07-01 00:00:00