Abstract:
:A total, 58 strains of Bacillus thuringiensis were isolated from soils of various regions in Korea. Serological tests showed that B. thuringiensis isolates represented 10 H serotypes, indicating a varied flora of B. thuringiensis. But the H serotypes did not have a significantly uneven distribution, ranging from 1 to 11 isolates. In toxicity tests, 35% of all isolates were toxic to lepidoptera, 20% were toxic to diptera, and 9% were non-toxic isolates. Especially, a large number of lepidopteran/dipteran-active isolates (36%) were found. Forty all lepidopteran-active isolates produced typical rhomboidial inclusions, and the remainder, which belong to dipteran-active and non-toxic isolates, were spherical in shape. In addition, lepidopteran/dipteran-active isolates produced rhomboidal or spherical inclusions. PCR analysis using cryI, II, III, IV, and V gene-specific primers showed that the frequency of the cryIC gene (57%) predominated, followed by the cryIA(b) (45%) and cryIIA genes (34%). But, the cryIE, cryIF, cryIII, cryIVC and cryV genes were not reactive. Several isolates had unusual PCR products and multiple insecticidal crystal protein genes. PCR results showed varied distribution of the cry-type gene. Seven isolates were selected for evaluation of novel activity according to the following criteria: flagellar serotypes, parasporal inclusion morphology, SDS-PAGE, plasmid DNA patterns, toxicity, and the cry-type gene in PCR analysis. Two isolates, named S333 (H7) and S225 (H7), among them synthesized PCR products of the cryIC gene, but the S333 isolate producing rhomboidal inclusion was toxic to both Plutella xylostella and Culex pipiens, whereas the S225 isolate having toxicity to only C. pipiens produced spherical inclusion.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Kim HS,Lee DW,Woo SD,Yu YM,Kang SKdoi
10.1007/s002849900363subject
Has Abstractpub_date
1998-09-01 00:00:00pages
195-200issue
3eissn
0343-8651issn
1432-0991journal_volume
37pub_type
杂志文章abstract::Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9608-3
更新日期:2010-10-01 00:00:00
abstract::Insecticidal proteins produced by strains of Bacillus thuringenesis are specific toward target pests. One of the Bt proteins, Cry 1Ac has been used successfully for controlling crop predation by polyphagous pests Helicoverpa armigera. Structurally, Bt proteins consist of three domains; domain I and III are fairly homo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9749-4
更新日期:2011-02-01 00:00:00
abstract::This study is to investigate and characterize the microbiota composition on eggshells from 3 different areas of Shaanxi province (Yulin, Hanzhong and Xi'an). The eggs were stored at 25 °C for 56 days and bacterial samples were collected from eggshells on day 0, 14, 28, 42 and 56. Denaturing gradient gel electrophoresi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02212-y
更新日期:2020-12-01 00:00:00
abstract::Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01570083
更新日期:1992-07-01 00:00:00
abstract::Pasteurella haemolytica (Ph) is the most important cause of the bovine acute fibrinohemorrhagic pneumonia that occurs in market stressed calves after shipment to feedyards. Recent characterization of neuraminidase production by these organisms has shown that all 16 serotypes produce an immunologically similar form of ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900372
更新日期:1998-10-01 00:00:00
abstract::Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both mo...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900163
更新日期:1997-03-01 00:00:00
abstract::The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF02601668
更新日期:1978-01-01 00:00:00
abstract::Pantoea beijingensis, a gram-negative pathogenic bacterium, causes soft rot disease in the fungus Pleurotus eryngii in China. However, the taxonomic classification of this pathogen is controversial due to close relationships between bacteria of the genera Pantoea and Erwinia. This study aimed to resolve the identity o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02253-3
更新日期:2021-01-01 00:00:00
abstract::A common activity in the global search for useful Cry toxins is the microscopic screening of bacterial colonies for the presence of Bacillus thuringiensis. High-throughput screens require that aliquots from large numbers of colonies be arrayed on a microscopic slide. However, precisely placing a small amount of bacter...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0870-2
更新日期:2015-09-01 00:00:00
abstract::The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-012-0243-z
更新日期:2013-01-01 00:00:00
abstract::Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encodin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4288-5
更新日期:2004-09-01 00:00:00
abstract::The influence of Bacillus thuringiensis subsp. kurstaki HD-1 spores upon the toxicity of purified Cry1Ab and Cry1C crystal proteins toward susceptible and BT-resistant Indianmeal moth (IMM, Plodia interpunctella) larvae was investigated. With susceptible larvae, HD-1 spores were toxic in the absence of crystal protein...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900074
更新日期:1996-07-01 00:00:00
abstract::Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1225-y
更新日期:2017-05-01 00:00:00
abstract::Biofilm-forming bacteria Pseudomonas aeruginosa is a common pathogen in mechanically ventilated newborns, which can cause life-threatening infections. Alginate of mucoid Pseudomonas aeruginosa biofilms is considered an important virulence factor which contributes to the resistance to antibiotics. Traditionally, ambrox...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9142-8
更新日期:2008-07-01 00:00:00
abstract::The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010111
更新日期:2000-09-01 00:00:00
abstract::Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9602-9
更新日期:2010-10-01 00:00:00
abstract::Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many species, including pigs, horses and humans. Biofilm formation is essential for pathogenesis, and the ability to resist antibiotic treatment results in difficult-to-treat and persistent infections. However, the ability o...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0574-z
更新日期:2014-09-01 00:00:00
abstract::The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3767-9
更新日期:2002-12-01 00:00:00
abstract::To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, p...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9867-7
更新日期:2011-05-01 00:00:00
abstract::Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900438
更新日期:1999-09-01 00:00:00
abstract::The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylor...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4244-4
更新日期:2004-07-01 00:00:00
abstract::Butterfly stain, common in Chilean tepa (Laureliopsis philippiana [Looser] Schodde) trees, appears in cross-section of the stem as a series of partially overlapping orange-brown arcs that in early stages resembles a butterfly. Bacterial isolates from stained tepa wood samples cultured under aerobic and anaerobic condi...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849910016
更新日期:2000-02-01 00:00:00
abstract::The natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, enviro...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0023-1
更新日期:2011-12-01 00:00:00
abstract::There is a region exhibiting a similarity of amino acid sequence near the carboxyl-terminal segment of each FAD-containing oxidoreductase. In this region, four amino acid residues-Thr, Ala, Gly, and Asp-are highly conserved. To determine the involvement of the four amino acid residues (Thr-469, Ala-476, Gly-478, and A...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3911-6
更新日期:2003-06-01 00:00:00
abstract::To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9960-y
更新日期:2011-08-01 00:00:00
abstract::Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9585-6
更新日期:2010-08-01 00:00:00
abstract::The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA,...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9698-y
更新日期:2011-01-01 00:00:00
abstract::Corrinoids in several diverse species of methanogens were quantified by a bioassay utilizingEscherichia coli 113-3, a corrinoid auxotroph. All five species examined contained >0.65 nmol corrinoid/mg dry cells when grown on H2/CO2 as carbon and energy source. The highest corrinoid levels (4.1 nmol/mg cells) were found ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF02602456
更新日期:1980-07-01 00:00:00
abstract::Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9347-x
更新日期:2009-04-01 00:00:00
abstract::Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-014-0651-3
更新日期:2014-11-01 00:00:00