Abstract：
:A common activity in the global search for useful Cry toxins is the microscopic screening of bacterial colonies for the presence of Bacillus thuringiensis. High-throughput screens require that aliquots from large numbers of colonies be arrayed on a microscopic slide. However, precisely placing a small amount of bacteria on a slide, and at a density that is useful for microscopic examination, is both difficult to achieve and time consuming. Herein we share a simple technique that utilizes a hooked wand and small polystyrene beads to quickly collect, and uniformly apply, aliquots of bacterial colonies onto gridded microscope slides in a manner optimal for viewing. If desired, libraries of examined bacteria can simultaneously be generated by discharging the beads into indexed multiwell plates. This simple and inexpensive method is robust, suitable for both light and phase contrast microscopy, and has been also used successfully to screen randomly mutated bacteria for phenotypic changes.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Ammons D,Rampersad Jdoi
10.1007/s00284-015-0870-2subject
Has Abstractpub_date
2015-09-01 00:00:00pages
412-4issue
3eissn
0343-8651issn
1432-0991journal_volume
71pub_type
杂志文章abstract：:Rhodomyrtus tomentosa leaf has been traditionally used to treat many infections. This plant species has been documented to possess a wide spectrum of pharmacological effects. This study aimed to determine the effects of Rhodomyrtus tomentosa leaf extract and its potent purified compound, rhodomyrtone, on Streptococcus...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02164-3
更新日期：2020-11-01 00:00:00
abstract：:The rates of urinary tract infection with multidrug-resistant (MDR) Escherichia coli have dramatically increased and the treatment of these infections with single and double antibiotic combinations became limited or ineffective. The present study aimed at finding effective antibiotic combinations against MDR uropathog...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01871-w
更新日期：2020-05-01 00:00:00
abstract：:The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9351-1
更新日期：2009-05-01 00:00:00
abstract：:Colonial aggregation of Microcystis plays a key role in bloom formation. Limited studies have been reported about effects of environmental factors on the aggregation of Microcystis. Calcium is an important chemical element in water system. In this study, we investigated the effects of a low- (0.015 g l⁻¹) and a high-c...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9762-7
更新日期：2011-02-01 00:00:00
abstract：:Antimicrobial-resistant Klebsiella pneumoniae represent a global public health concern. K. pneumoniae strains isolated during 2010 and 2014-2016 within a single hospital of Molise Region, Central Italy, were analyzed testing antimicrobial susceptibility, clonality by pulsed-field gel electrophoresis (PFGE) and random ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1475-3
更新日期：2018-08-01 00:00:00
abstract：:Expression from the secA gene, encoding a key component of the general secretory pathway of Escherichia coli, is influenced by the secretion status of the cell, autogenous translational repression, and translational coupling to the upstream gene, X. SecA binds to its mRNA in a region overlapping its ribosome binding s...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900413
更新日期：1999-02-01 00:00:00
abstract：:A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0693-6
更新日期：2015-01-01 00:00:00
abstract：:One hundred fifty-five isolates of Bipolaris sorokiniana of wheat were studied for their morphopathological characterization. These isolates were grouped in five categories--black, brown/dull black, gray cottony growth, dull white/greenish black, and white--on the basis of their growth pattern. The frequency of the bl...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0035-z
更新日期：2007-08-01 00:00:00
abstract：:A NaCl(r) mutant of the diazotrophic cyanobacterium Anabaena variabilis has been isolated by NTG mutagenesis and selection for NaCl resistance. The NaCl(r) strain has been characterized with respect to its mechanism of NaCl tolerance and regulation of Na(+) influx. NaCl(r) strain exhibits low Na(+) influx, accumulated...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010186
更新日期：2001-02-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This wo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9608-3
更新日期：2010-10-01 00:00:00
abstract：:Lectins LI and LII, localized on the surface of the nitrogen-fixing soil bacterium Bacillus polymyxa 1460, were shown to possess proteolytic activity. A relationship was found between the proteolytic and hemagglutinating activities of the lectins. Blocking of hemagglutinating activity with specific carbohydrate hapten...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010128
更新日期：2000-10-01 00:00:00
abstract：:A screen of thermophilic lactic acid bacteria identified Streptococcus thermophilus strain ST110 as the putative producer of a bacteriocin with high level of activity against pediococci. Thermophilin 110 was isolated from culture supernatant after 16 h of growth and partially purified by a chloroform extraction proced...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4540-7
更新日期：2005-09-01 00:00:00
abstract：:Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both mo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900163
更新日期：1997-03-01 00:00:00
abstract：:A novel isolate of a bacterium, capable of degrading trichloroethylene (TCE) and growing on this as the sole carbon source is reported. The test strain was isolated by an enrichment technique with trichloroethylene as the substrate. The isolated strain belongs to the genus Bacillus. The practical utility of cleaning u...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9427-6
更新日期：2009-09-01 00:00:00
abstract：:Previous studies have established that psyllids (Hemiptera, Psylloidea) contain primary endosymbionts, designated as Carsonella ruddii, which cospeciate with the psyllid host. This association appears to be the consequence of a single infection of a psyllid ancestor with a bacterium. Some psyllids may have additional ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010138
更新日期：2000-10-01 00:00:00
abstract：:Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1272-4
更新日期：2017-08-01 00:00:00
abstract：:Termites are global pests and can cause serious damage to buildings, crops, and plantation forests. The symbiotic intestinal flora plays an important role in the digestion of cellulose and nitrogen in the life of termites. Termites and their symbiotic microbes in the gut form a synergistic system. These organism work ...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-018-1502-4
更新日期：2019-06-01 00:00:00
abstract：:Reindeer (Rangifer tarandus tarandus) may include large proportions of lichens in their winter diet. These dietary lichens are rich in phenolic secondary compounds, the most well-known being the antimicrobial usnic acid. Previous studies have shown that reindeer host rumen bacteria resistant to usnic acid and that usn...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0534-7
更新日期：2014-06-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake in the provided KEMB accession number. ...
journal_title：Current microbiology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00284-018-1592-z
更新日期：2019-01-01 00:00:00
abstract：:Actinomycetes are a wide group of Gram positive prokaryotes, the soil being their most characteristic habitat, where they play important ecological functions. Their immense biotechnological potential as producers of bioactive molecules of great commercial and industrial interest is exemplified by most antibiotics for ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02030-2
更新日期：2020-09-01 00:00:00
abstract：:There is a region exhibiting a similarity of amino acid sequence near the carboxyl-terminal segment of each FAD-containing oxidoreductase. In this region, four amino acid residues-Thr, Ala, Gly, and Asp-are highly conserved. To determine the involvement of the four amino acid residues (Thr-469, Ala-476, Gly-478, and A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3911-6
更新日期：2003-06-01 00:00:00
abstract：:Ansamycins is a group of type I polyketides characterized by the unique starter unit 3-amino-5-hydroxybenzoic acid. This family of secondary metabolites shows diverse biological activities, well-known members of which include rifamycin, geldanamycin, and maytansine. Previously, we isolated an AHBA synthase gene-positi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0798-6
更新日期：2015-06-01 00:00:00
abstract：:Forty-eight isolates resistant to at least two antibiotics were selected from 53 antibiotic-resistant enterococci from chicken and pig meat and faeces and analysed for specific resistance determinants. Of the 48 multidrug-resistant (MDR) strains, 31 were resistant to two antibiotics (29 to erythromycin and tetracyclin...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9880-x
更新日期：2011-05-01 00:00:00
abstract：:To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9334-2
更新日期：2009-03-01 00:00:00
abstract：:DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) is one of the pesticides that are hazardous for the environment and human health. Effective environmental-friendly treatment using co-cultures of fungi and bacteria is needed. In this study, the bacteria Bacillus subtilis at various volumes of 1, 3, 5, 7, and 10 mL (...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1286-y
更新日期：2017-09-01 00:00:00
abstract：:We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia, Cardinium, Rickettsia, Spiroplasma, Arsenophonus, and Hamiltonella. Our research, for the first time, revealed the presence of Cardinium in Mic...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01717-5
更新日期：2019-09-01 00:00:00
abstract：:To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested. For dispersion, stirring with cation exch...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/pl00006809
更新日期：1999-06-01 00:00:00
abstract：:Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and posse...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0717-2
更新日期：2015-02-01 00:00:00
abstract：:This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9038-z
更新日期：2008-01-01 00:00:00
abstract：:The duodenal ulcer promoting gene (dupA), located in the plasticity region of Helicobacter pylori (H. pylori), is predicted to form a type IV secretory system (T4SS) with vir genes around dupA. In the study, we investigated the association between the dupA cluster status and the virulence of H. pylori in a littoral re...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-015-0812-z
更新日期：2015-07-01 00:00:00
