Involvement of glycine and aspartate residues in the binding capacity of FAD in the NADH dehydrogenase from an alkaliphilic Bacillus.

abstract：:A genetic fingerprinting analysis of Caribbean isolates of M. avium complex (MAC) from AIDS patients by a Southern blotting technique after Pstl digestion with nonradioactive DNA probes coding for single-copy sequences DT1 and DT6 was performed. In parallel, a selective amplification of a 187-bp fragment within the DT...

journal_title：Current microbiology

authors： Sola C,Devallois A,Goh KS,Legrand E,Rastogi N

更新日期：1996-12-01 00:00:00

abstract：:The thermophilic fungus Thermomyces lanuginosus, which is able to use dextran as primary carbon source for growth, excreted during the early phases of growth an enzyme activity capable of degrading dextran. The activity peaked at 22 h and decreased rapidly after the culture entered the stationary phase, probably cause...

journal_title：Current microbiology

authors： Jensen B,Olsen J

更新日期：1996-09-01 00:00:00

abstract：:In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...

journal_title：Current microbiology

authors： Lienemann T,Pitkänen T,Antikainen J,Mölsä E,Miettinen I,Haukka K,Vaara M,Siitonen A

更新日期：2011-04-01 00:00:00

abstract：:Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacter...

journal_title：Current microbiology

authors： Kasana RC,Sharma UK,Sharma N,Sinha AK

更新日期：2007-06-01 00:00:00

abstract：:A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...

journal_title：Current microbiology

authors： Zhang H,Zhao HZ,Liu J,Qin W,Huang X

更新日期：2020-11-01 00:00:00

abstract：:Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological control activities, promote plant health or cause plant disease. Here, we present the draft genome sequence of the kiwifrui...

journal_title：Current microbiology

authors： Zhang L,Wu Z,Wang X,Tan G,Song J

更新日期：2019-05-01 00:00:00

abstract：:The objective of this study was to evaluate the ability of the human colonic bacterium Bacteroides ovatus V975 to ferment fenugreek fiber (Fenufibers), psyllium husk (Metamucil), and wheat bran (Wheat Chex). Strain V975 was incubated in basal medium that contained 0.1 g of each fiber source for 0, 24, or 48 h. Little ...

journal_title：Current microbiology

authors： Al-Khaldi SF,Martin SA,Prakash L

更新日期：1999-10-01 00:00:00

abstract：:A bacterium capable of utilizing p-cresol as sole source of carbon and energy was isolated from soil and identified as a Bacillus species. The organism also utilized phenol, o-cresol, m-cresol, 4-hydroxybenzoic acid, and gentisic acid as growth substrates. The organism degraded p-cresol to 4-hydroxybenzoic acid, which...

journal_title：Current microbiology

authors： Tallur PN,Megadi VB,Kamanavalli CM,Ninnekar HZ

更新日期：2006-12-01 00:00:00

abstract：:Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...

journal_title：Current microbiology

authors： Alam MJ,Renter D,Taylor E,Mina D,Moxley R,Smith D

更新日期：2009-04-01 00:00:00

abstract：:The duodenal ulcer promoting gene (dupA), located in the plasticity region of Helicobacter pylori (H. pylori), is predicted to form a type IV secretory system (T4SS) with vir genes around dupA. In the study, we investigated the association between the dupA cluster status and the virulence of H. pylori in a littoral re...

journal_title：Current microbiology

authors： Wang MY,Shao C,Li J,Yang YC,Wang SB,Hao JL,Wu CM,Gao XZ,Shao SH

更新日期：2015-07-01 00:00:00

abstract：:Endophytes are beneficial microbes that reside intercellularly inside the plants. Interaction of endophytes with the host plants and their function within their host are important to address ecological relevance of endophyte. Four endophytic bacteria OS-9, OS-10, OS-11, and OS-12 were isolated from healthy leaves of O...

journal_title：Current microbiology

authors： Tiwari R,Kalra A,Darokar MP,Chandra M,Aggarwal N,Singh AK,Khanuja SP

更新日期：2010-03-01 00:00:00

abstract：:Amino acid residues in the active site of quinoline 2-oxidoreductase (Qor) that are deemed important for substrate binding and turnover were replaced by site-directed mutagenesis. The apparent k(cat) values for quinoline were reduced 2.4-, 38-, 40-, and 199-fold in the protein variants QorA259G, QorW331G, QorV373A, an...

journal_title：Current microbiology

authors： Purvanov V,Fetzner S

更新日期：2005-04-01 00:00:00

abstract：:The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira inte...

journal_title：Current microbiology

authors： Hartwig DD,Seixas FK,Cerqueira GM,McBride AJ,Dellagostin OA

更新日期：2011-04-01 00:00:00

abstract：:Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...

journal_title：Current microbiology

authors： Gírio FM,Roseiro JC,Silva AI

更新日期：1998-06-01 00:00:00

abstract：:Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the gre...

journal_title：Current microbiology

authors： Sun J,McFarland M,Boettner D,Panepinto J,Rhodes JC,Askew DS

更新日期：2001-01-01 00:00:00

abstract：:Gram-negative bacteria are generally more tolerant to disinfectants than Gram-positive bacteria due to outer membrane (OM) barrier, but the tolerant mechanism is not well characterized. We have utilized comparative proteomic methodologies to characterize the OM proteins of E. coli K-12 K99+ in response to phenol stres...

journal_title：Current microbiology

authors： Zhang DF,Li H,Lin XM,Wang SY,Peng XX

更新日期：2011-03-01 00:00:00

abstract：:Four cell lysis methods (NaOH-SDS solubilization, French press treatment, sonication, mutanolysin treatment) and three methods of protein assays (Lowry, Bradford, Pierce) were studied for their applicability to determination of cell volume in Clostridium perfringens NCTC 8798 cell suspensions. Protein contents were hi...

journal_title：Current microbiology

authors： Guerlava P,Izac V,Tholozan JL

更新日期：1998-03-01 00:00:00

abstract：:A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain YIM132180T, was isolated from a Lepraria sp. lichen collected from Pu'er, Yunnan Province, China. The strain grew at 15-35 °C (optimum, 25-28 °C), at 0-2% (w/v) NaCl (optimum, 0-1%) and at pH 6.0-9.0 (optimum, pH 7.0). The 16S rRNA ge...

journal_title：Current microbiology

authors： Zhang K,Jiang LQ,Wang LS,An DF,Lang L,Li GD,Wang XY,Shi SB,Li QY,Jiang CL,Jiang Y

更新日期：2020-02-01 00:00:00

abstract：:Fifty-one Bifidobacterium strains were isolated from the feces of healthy adults (30-40 years old) and seniors (older than 70 years of age). B. adolescentis, B. breve, B. infantis, and B. longum were isolated from the healthy adults and B. adolescentis and B. longum from elderly subjects. The tested bacteria bound, in...

journal_title：Current microbiology

authors： He F,Ouwehand AC,Isolauri E,Hosoda M,Benno Y,Salminen S

更新日期：2001-11-01 00:00:00

abstract：:The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patie...

journal_title：Current microbiology

authors： Cho YS,Schiller NL,Oh KH

更新日期：2008-12-01 00:00:00

abstract：:Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml(-1) in SF and 21.5 U g(-1) in SSF; these values were approximately ...

journal_title：Current microbiology

authors： De Azeredo LA,De Lima MB,Coelho RR,Freire DM

更新日期：2006-10-01 00:00:00

abstract：:Thiothrix spp., sulfide-oxidizing filamentous bacteria, were found to be a principal bacterial component of aquatic biofilms causing biofouling in selected municipal water storage tanks, private wells, and drip irrigation systems in Florida. Treatments of up to 200 ppm chlorine in the affected systems could not preven...

journal_title：Current microbiology

authors： Brigmon RL,Martin HW,Aldrich HC

更新日期：1997-09-01 00:00:00

abstract：:Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...

journal_title：Current microbiology

authors： Castellanos MI,Chauvet A,Deschamps A,Barreau C

更新日期：1996-08-01 00:00:00

abstract：:The histidine decarboxylase gene cluster of Morganella morganii DSM30146(T) was sequenced, and four open reading frames, named hdcT1, hdc, hdcT2, and hisRS were identified. Two putative histidine/histamine antiporters (hdcT1 and hdcT2) were located upstream and downstream the hdc gene, codifying a pyridoxal-P dependen...

journal_title：Current microbiology

authors： Ferrario C,Borgo F,de Las Rivas B,Muñoz R,Ricci G,Fortina MG

更新日期：2014-03-01 00:00:00

abstract：:The isolate RNP4 obtained from a long-term tannery waste contaminated soil was characterized and presumptively identified as Pseudomonas sp. The strain RNP4 tolerated concentrations up to 450 mg Cr(6+)/L on a Luria-Bartani (LB) agar medium and reduced a substantial amount of Cr(6+) to Cr(3+) in the LB liquid medium. T...

journal_title：Current microbiology

authors： Rajkumar M,Nagendran R,Lee KJ,Lee WH

更新日期：2005-05-01 00:00:00

abstract：:The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whe...

journal_title：Current microbiology

authors： Reddy YC,Venkateswerlu G

更新日期：2002-12-01 00:00:00

abstract：:The glucose 1-phosphate uridylyltransferase (GalU) is absolutely required for the biosynthesis of capsular polysaccharide, the sine qua non virulence factor of Streptococcus pneumoniae. The pneumococcal GalU protein was overexpressed in Escherichia coli, and purified. GalU showed a pI of 4.23, and catalyzed the revers...

journal_title：Current microbiology

authors： Bonofiglio L,García E,Mollerach M

更新日期：2005-10-01 00:00:00

abstract：:Oxylipin metabolism represents one of the important hormonal and defensive mechanisms employed by plants, algae, or animals. It begins mostly with the reaction of lipoxygenases (LOXs), which catalyze the oxygenation of polyunsaturated fatty acids to form the corresponding hydroperoxides. At present, little information...

journal_title：Current microbiology

authors： Koeduka T,Kajiwara T,Matsui K

更新日期：2007-04-01 00:00:00

abstract：:With human saliva as an inoculum, microcosm dental plaques were grown on dental amalgam in a constant-depth film fermentor (CDFF) in the presence (suc +ve) and absence (suc -ve) of sucrose. The biofilms were then exposed to 0.2% chlorhexidine gluconate (CHG) for 1, 5, or 60 min and the survivors enumerated. Suc +ve bi...

journal_title：Current microbiology

authors： Wilson M,Patel H,Noar JH

更新日期：1998-01-01 00:00:00

abstract：:The natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, enviro...

journal_title：Current microbiology

authors： Arboleda Valencia JW,Valencia Jiménez A,Gonçalves de Siqueira F,Dussan Medina K,Restrepo Franco GM,Filho EX,Siegfried BD,Grossi-de-Sa MF

更新日期：2011-12-01 00:00:00