Enzyme activity of lectins from the nitrogen-fixing soil bacterium Bacillus polymyxa.

Abstract:

:Lectins LI and LII, localized on the surface of the nitrogen-fixing soil bacterium Bacillus polymyxa 1460, were shown to possess proteolytic activity. A relationship was found between the proteolytic and hemagglutinating activities of the lectins. Blocking of hemagglutinating activity with specific carbohydrate haptens led to significant changes in the enzyme activity of both lectins. When lectin activity was blocked with glucuronic acid and fructose-1, 6-diphosphate, the proteolytic activity of both LI and LII declined, whereas incubation with d-galactosamine and d-glucosamine promoted increases in the proteolytic activity of LII. This study proposes that the molecules of the B. polymyxa lectins may have two centers on their surfaces: one responsible for lectin activity and the other for proteolytic activity.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Mel'nikova UY,Karpunina LV,Ostakhina NV,Ignatov VV

doi

10.1007/s002840010128

keywords:

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

246-9

issue

4

eissn

0343-8651

issn

1432-0991

pii

10.1007/s002840010128

journal_volume

41

pub_type

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