Abstract:
:Aminopeptidases (APN) may play a role in host colonization of M. gallinarum. Characterization of endogenous APN activity suggests that the leucine APN (LAP) of M. gallinarum is a metallo-aminopeptidase activated by Mn2+ and is present in the cytosol and possibly associated with the inner leaflet of the membrane. A 1.36-kb open reading frame (ORF) identified from overlapping genomic phage clones showed 68% nucleotide identity and 51% amino acid identity with the M. salivarium LAP gene. This ORF is expressed as a 1.5-kb monocistronic transcript and is present as a single copy in M. gallinarum. This gene sequence was modified to account for codon usage, and expression in E. coli produced a 51-kDa protein, which compares well with the product predicted from the ORF. This ORF is a strong candidate for contributing the LAP activity of M. gallinarum protein extracts.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Wan X,Branton SL,Hanson LA,Pharr GTdoi
10.1007/s00284-003-4098-1keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
32-8issue
1eissn
0343-8651issn
1432-0991journal_volume
48pub_type
杂志文章abstract::Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0133-8
更新日期:2006-03-01 00:00:00
abstract::Grasslands are suffering from long-term overgrazing because of the population inflation. Furthermore, nitrous oxide (N2O) is a major greenhouse gas that also depletes stratospheric ozone. However, the emission rate of grassland N2O and underlying mechanisms remained unclear under different grazing intensities. We cond...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-019-01668-x
更新日期:2019-05-01 00:00:00
abstract::The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002840010111
更新日期:2000-09-01 00:00:00
abstract::A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterizatio...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900470
更新日期:1999-12-01 00:00:00
abstract::Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to s...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0009-z
更新日期:2011-12-01 00:00:00
abstract::Soil microcosm experiments were used to investigate the effects on growth and activity of soil microorganisms of an herbicide combination (60% bromoxynil + 3% prosulfuron) frequently used to provide a broad spectrum control of weed species. Culturable aerobic bacteria, fungi, and actinomycetes, the fundamental groups ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0116-4
更新日期:2006-09-01 00:00:00
abstract::An isolate of Lactococcus lactis from fermented milk was found to produce a bacteriocin peptide. The isolate could grow in a medium with an initial pH of 11.0, in which it produced the bacteriocin extracellularly at the highest level. The level of the bacteriocin in the medium increased in parallel to the bacterial gr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-4545-2
更新日期:2005-09-01 00:00:00
abstract::The limitation of phosphate concentrations in effluents is of international concern because of the risk of eutrophication.Acinetobacter spp. have often been isolated from activated sludge plants exhibiting enhanced phosphate removal. The ability to remove phosphate from mixed liquor byAcinetobacter isolates and refere...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01576016
更新日期:1993-09-01 00:00:00
abstract::This study was taken up with a view to generate basic information on spore hardiness to ethanol in various Bacillus species and related genera, and to assess the effectiveness of different levels of ethanol as a bacterial disinfectant. Predominantly spore-bearing cultures of five Bacillus spp. (B. pumilus, B. subtilis...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-0040-0
更新日期:2012-02-01 00:00:00
abstract::Pseudomonas aeruginosa is a serious pathogen involved in nosocomial infections. Its pathogenicity is owed to rich production of virulence factors (VIFs) regulated by several complex hierarchical signal systems depending on environmental conditions, medium composition, and the presence of certain active compounds in it...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0441-7
更新日期:2007-04-01 00:00:00
abstract::A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02210-0
更新日期:2020-11-01 00:00:00
abstract::Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians re...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1272-4
更新日期:2017-08-01 00:00:00
abstract::Leclercia sp. W6 and W17, which belong to the Enterobacteriaceae, were isolated from a stomach sample from a 78-year-old female gastric cancer patient, and genomic sequencing and analysis were performed. The genome of Leclercia sp. W6 consists of one chromosome with a size of 4,945,486 bp, while that of Leclercia sp. ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02075-3
更新日期:2020-10-01 00:00:00
abstract::The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected str...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1428-2
更新日期:2018-05-01 00:00:00
abstract::A novel bacterium, designated U33(T), was isolated from a soil sample collected in Mykhailyky, Poltavs'ka oblast, Ukraine. The bacterium was aerobic, Gram-positive, spore-forming, and consists of motile rods. The taxonomic position of strain U33(T) was studied by a polyphasic approach, and the results clearly showed t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0930-7
更新日期:2016-02-01 00:00:00
abstract::(13)CNMR and (1)HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells. This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3944-x
更新日期:2003-07-01 00:00:00
abstract::Invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. Candida albicans is the main pathological agent followed by Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis. These pathogens colonize different host tissues in humans as they are able t...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-014-0651-3
更新日期:2014-11-01 00:00:00
abstract::A novel isolate of a bacterium, capable of degrading trichloroethylene (TCE) and growing on this as the sole carbon source is reported. The test strain was isolated by an enrichment technique with trichloroethylene as the substrate. The isolated strain belongs to the genus Bacillus. The practical utility of cleaning u...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9427-6
更新日期:2009-09-01 00:00:00
abstract::A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0495-2
更新日期:2014-04-01 00:00:00
abstract::Favre and coworkers (Favre et al., Biochimie 73:235-244, 1991) previously reported that the Bordetella pertussis recA gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in Escherichia coli RecA- mutants. Reexamination of these phenotypes demonstrated that, ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900029
更新日期:1996-03-01 00:00:00
abstract::The main studies on the corrosion of metals induced by microorganisms are directed only to the surface of the metal, without considering the presence of welds between these surfaces. For this reason, we evaluated the difference of microbial community grown in carbon steel coupons, and two different types of welds, E70...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01898-4
更新日期:2020-06-01 00:00:00
abstract::Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-006-0600-x
更新日期:2007-08-01 00:00:00
abstract::Forty-eight isolates resistant to at least two antibiotics were selected from 53 antibiotic-resistant enterococci from chicken and pig meat and faeces and analysed for specific resistance determinants. Of the 48 multidrug-resistant (MDR) strains, 31 were resistant to two antibiotics (29 to erythromycin and tetracyclin...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9880-x
更新日期:2011-05-01 00:00:00
abstract::Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the c...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-018-1444-x
更新日期:2018-06-01 00:00:00
abstract::One of the main requirements for probiotics is their ability to survive during passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibito...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1017-9
更新日期:2016-06-01 00:00:00
abstract::There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suita...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1429-1
更新日期:2018-06-01 00:00:00
abstract::Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main fl...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-016-1094-9
更新日期:2016-10-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (E. coli) O157:H7, a gram-negative bacteria identified as a foodborne pathogen causing severe disease is of great concern worldwide. The pathogenicity of E. coli O157:H7 is due to the presence of some virulence factors and its ability to form biofilm which resist antimicrobial compou...
journal_title:Current microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00284-017-1314-y
更新日期:2017-12-01 00:00:00
abstract::Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900462
更新日期:1999-11-01 00:00:00
abstract::We have shown previously that Spo0A approximately P-dependent sinIR operon expression was substantially down-regulated in abrB null mutant backgrounds. In this report, we show that loss of function mutations in abrB also cause phosphorelay gene expression to be down regulated. abrB null mutations caused diminished veg...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4186-2
更新日期:2004-04-01 00:00:00