Abstract：
:Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α(rh) can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α(rh). These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α(rh). We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Gong J,Shen XH,Qiu H,Chen C,Yang RGdoi
10.1007/s00284-011-0009-zsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
531-7issue
6eissn
0343-8651issn
1432-0991journal_volume
63pub_type
杂志文章abstract：:The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. The gene of ferred...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-9025-4
更新日期：2007-12-01 00:00:00
abstract：:A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0-8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular pola...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01823-4
更新日期：2020-02-01 00:00:00
abstract：:The establishment of human flora-associated animal models allows the in vivo manipulation of host, microbial, and environmental parameters to influence the gut microbial community. However, it is difficult to simulate infant gut microbiota in germ-free animals because of the variation and dynamic state of infant micro...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0360-3
更新日期：2013-09-01 00:00:00
abstract：:Multi-drug resistance and transfer of mobile genetic elements among enteric pathogens is being reported to have increased rapidly. Commensal Escherichia coli was previously known to acquire mobile genetic elements from other genus/species. E. coli is also capable of disseminating these elements containing antimicrobia...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01676-x
更新日期：2019-06-01 00:00:00
abstract：:The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined. The viable numbers ranged between 10(4) and 10(6) cell g(-1) cyanobacterial biomass. A total of 112 morphotypes, representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated fr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0243-z
更新日期：2013-01-01 00:00:00
abstract：:AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus ...
journal_title：Current microbiology
pub_type： 信件
doi：10.1007/s00284-013-0477-4
更新日期：2014-03-01 00:00:00
abstract：:The cluster of genes encoding components of the progenitor botulinum neurotoxin complex has been mapped and cloned in Clostridium botulinum type G strain ATCC 27322. Determination of the nucleotide sequence of the region has revealed open reading frames encoding nontoxic components of the complex, upstream of the gene...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900240
更新日期：1997-10-01 00:00:00
abstract：:Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9572-y
更新日期：2010-07-01 00:00:00
abstract：:Streptococcus bovis is commonly present in the rumen, but strains of S. bovis have also occasionally been isolated from human blood or fecal samples. Studies were undertaken with 16s rRNA gene sequences and DNA hybridizations to define the genetic relationships between these two groups of strains. Ruminal strains were...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00314583
更新日期：1995-11-01 00:00:00
abstract：:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors tha...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900462
更新日期：1999-11-01 00:00:00
abstract：:Leucoagaricus gongylophorus, the fungus cultured by the leaf-cutting ant Atta sexdens, produces polysaccharidases that degrade leaf components by generating nutrients believed to be essential for ant nutrition. We evaluated pectinase, amylase, xylanase, and cellulase production by L. gongylophorus in laboratory cultur...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0431-1
更新日期：2006-07-01 00:00:00
abstract：:Interactions between Bacillus anthracis (B. anthracis) and host cells are of particular interest given the implications of anthrax as a biological weapon. Inhaled B. anthracis endospores encounter alveolar macrophages as the first line of defense in the innate immune response. Yet, the consequences of this interaction...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9654-x
更新日期：2010-12-01 00:00:00
abstract：:The goal of this study was to evaluate how two new hydrolysates from poultry by-products act on ten lactobacilli growth kinetics when supplemented to the growth medium. These effects were compared with ones induced by two most common commercial hydrolysates, i.e., tryptone and peptone. Growth medium, supplemented with...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-013-0445-z
更新日期：2014-01-01 00:00:00
abstract：:Enterotoxigenic Escherichia coli causes diarrhea by producing several virulence factors including heat-labile enterotoxin (LT). LT is maximally expressed at 37 degrees C. The histone-like nucleoid structuring protein (H-NS) appears to inhibit LT expression by binding to a downstream regulatory element (DRE) at low tem...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-004-4282-y
更新日期：2004-11-01 00:00:00
abstract：:The mechanisms used by Campylobacter jejuni to colonize the (chicken) intestinal tract have not been defined. In this study, we obtained evidence that in the presence of chicken serum and mucus, C. jejuni secreted proteins that may play a role in the colonization of chicken gut (Campylobacter invasion antigen = Cia). ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0295-z
更新日期：2007-03-01 00:00:00
abstract：:Betel vine is an edible creeper used in folk medicine to aid digestion since time immemorial. It is an ideal candidate deemed for the bioprospection of endophytic microorganisms with valuable attributes. This study aimed at the characterization of potential bacteria from fermented betel leaves. We report the presence ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02270-2
更新日期：2021-01-01 00:00:00
abstract：:The immunosuppressant leflunomide (LFM) inhibits the growth of cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of eukaryotic microorganism, Saccharomyces cerevisiae. As a first step to elucidate the molecular mechanism of LFM action in human, yeast gene which suppresses the an...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010267
更新日期：2001-08-01 00:00:00
abstract：:Nitric oxide synthase (NOS), the enzyme responsible for the production of endogenous nitric oxide from arginine, has been recently discovered in a number of Gram-positive bacteria. While bacterial NOS has been implicated in mediating nitrosative stress, much remains unknown about the functional role of endogenous nitr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0039-6
更新日期：2012-02-01 00:00:00
abstract：:Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0349-2
更新日期：2007-07-01 00:00:00
abstract：:Bacteria acquire new DNA in a process known as horizontal gene transfer (HGT). To investigate the evolutionary impact of this transfer of DNA, various methods have been developed to detect past HGT events. For example, codon usage-based methods detect the presence of transferred genes by identifying atypical patterns ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0205-5
更新日期：2012-11-01 00:00:00
abstract：:The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment sam...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-0056-5
更新日期：2012-03-01 00:00:00
abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF01577445
更新日期：1994-11-01 00:00:00
abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9288-4
更新日期：2009-02-01 00:00:00
abstract：:The organization of the clusters of genes encoding proteins of the botulinum neurotoxin (BoNT) progenitor complex was elucidated in a strain of Clostridium botulinum producing type B and F neurotoxins. With PCR and sequencing strategies, the type B BoNT-gene cluster was found to be composed of genes encoding BoNT/B, n...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900384
更新日期：1998-11-01 00:00:00
abstract：:Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294521
更新日期：1995-01-01 00:00:00
abstract：:Sheath rot disease of rice caused by Sarocladium oryzae (Sawada) (=Acrocylindrium oryzae, Sawada) has become an important production constraint in all rice-growing countries. Pathogenicity, phytotoxic metabolites, and random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4509-6
更新日期：2005-06-01 00:00:00
abstract：:Several rhizobial strains possess the ability to modulate leguminous plants ethylene levels by producing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase. While the effect of ACC deaminase has been studied in several rhizobia belonging to the Alphaproteobacteria class, not much is understood about its impa...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1474-4
更新日期：2018-08-01 00:00:00
abstract：:Endophytes are beneficial microbes that reside intercellularly inside the plants. Interaction of endophytes with the host plants and their function within their host are important to address ecological relevance of endophyte. Four endophytic bacteria OS-9, OS-10, OS-11, and OS-12 were isolated from healthy leaves of O...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-009-9520-x
更新日期：2010-03-01 00:00:00
abstract：:Enterovirus 71 (EV71) is the main pathogen of the hand, foot, and mouth disease. It was firstly isolated from sputum specimens of infants with central nervous system diseases in California in 1969, and has been repeatedly reported in various parts of the world, especially in the Asia-Pacific region. EV71 3C protein is...
journal_title：Current microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00284-020-02082-4
更新日期：2020-09-01 00:00:00
abstract：:The crystal morphology and the profiles of genes encoding protein toxins (Cry and Cyt) were analyzed in 12 Bacillus thuringiensis strains isolated during epizootics in laboratory culture lines of Cydia pomonella, 2 isolates cultured from Leucoma salicis larvae, and 9 reference strains. Epizootic isolates produced crys...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-007-0085-2
更新日期：2007-09-01 00:00:00
