Abstract：
:The enumeration of Archaea in deep-sea sediment samples is still limited, although different methodological procedures have been applied. Among these, catalysed reporter deposition-fluorescence in situ hybridisation (CARD-FISH) technique is a promising tool for estimation of archaeal abundance in deep-sea sediment samples. Comparing different permeabilisation treatments, the best results obtained both on archaeal pure cultures and on natural assemblages were with hydrochloric acid (0.1 M) and proteinase K (0.004 U/ml) treatments. The application of CARD-FISH on deep-sea sediments revealed that Archaea reach up to 41% of total prokaryotic cells. Specific probes for planktonic Archaea showed that marine Crenarchaea dominated archaeal seafloor communities. No clear bathymetric trends were observed for archaeal abundances and the morphology of continental margin (slope vs. canyon) seems not to have a direct influence on archaeal relative abundances. The site-specific sediment habitat-both abiotic environmental setting and sedimentary organic matter quality-explain up to 65% of variance of archaeal, crenarchaeal and euryarchaeal relative abundance, suggesting a wide ecophysiological adaptation to deep-sea benthic ecosystems. The findings demonstrate that Archaea are an important component of benthic microbial assemblages so far neglected, and hence they lay the groundwork for more focused research on their ecological importance in the functioning of deep-sea benthic ecosystems.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Molari M,Manini Edoi
10.1007/s00284-011-0056-5subject
Has Abstractpub_date
2012-03-01 00:00:00pages
242-50issue
3eissn
0343-8651issn
1432-0991journal_volume
64pub_type
杂志文章abstract：:An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02130-z
更新日期：2020-10-01 00:00:00
abstract：:The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3886-3
更新日期：2003-04-01 00:00:00
abstract：:Bacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, and chikungunya. Previous work has shown that Cry4B binds to sever...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-012-0178-4
更新日期：2012-10-01 00:00:00
abstract：:Antibiotics are generally applied for treatment or as subtherapeutic agents to overcome diseases caused by pathogenic bacteria including Escherichia coli, Salmonella and Enterococcus species in poultry. However, due to their possible adverse effects on animal health and to maintain food safety, probiotics, prebiotics,...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-020-02091-3
更新日期：2020-09-01 00:00:00
abstract：:Bacillus sp. B25 is an effective biocontrol agent against the maize pathogenic fungus Fusarium verticillioides (Fv). Previous in vitro assays have shown that B25 has protease, glucanase, and chitinase activities and siderophores production; however, specific mechanisms by which B25 controls Fv are still unknown. To de...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-017-1372-1
更新日期：2018-03-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-011-9939-8
更新日期：2011-07-01 00:00:00
abstract：:The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45 degrees C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37 degrees C. The invertase activity was significantly inhibited by bivalent meta...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910036
更新日期：2000-03-01 00:00:00
abstract：:Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. dril...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0565-0
更新日期：2014-08-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-4540-7
更新日期：2005-09-01 00:00:00
abstract：:PCR has been widely used to identify cry-type genes, to determine their distribution, to detect new such genes and to predict insecticidal activities. We describe here a molecular approach to analyze the genetic diversity of B. thuringiensis cry-like genes based on denaturing gradient gel electrophoresis (DGGE). This ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-010-9776-1
更新日期：2011-03-01 00:00:00
abstract：:Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for inciden...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9243-4
更新日期：2008-12-01 00:00:00
abstract：:A moderately psychrophilic bacterium Corynebacterium paurometabolum MTCC 6841 (gram positive, short rod type) producing extracellular alkaline lipase was isolated from Lake Naukuchiatal, Uttaranchal, India. The bacterium was able to grow within a broad range of pH (5-10). Soyabean oil and olive oil served as the best ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0224-6
更新日期：2006-05-01 00:00:00
abstract：:By cloning and sequencing specific randomly amplified polymorphic DNA (RAPD) products, we have developed pairs of PCR primers that can be used to detect Xylella fastidiosa in general, and X. fastidiosa that cause citrus variegated chlorosis (CVC) specifically. We also identified a CVC-specific region of the X. fastidi...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/BF00294703
更新日期：1995-12-01 00:00:00
abstract：:Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further e...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-005-0218-4
更新日期：2006-02-01 00:00:00
abstract：:Thiothrix spp., sulfide-oxidizing filamentous bacteria, were found to be a principal bacterial component of aquatic biofilms causing biofouling in selected municipal water storage tanks, private wells, and drip irrigation systems in Florida. Treatments of up to 200 ppm chlorine in the affected systems could not preven...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900233
更新日期：1997-09-01 00:00:00
abstract：:The growth-inhibitory effect of 23 carbohydrate monoesters synthesized by lipases and proteases were assayed to obtain antibacterial agents that suppress the cell growth of Streptococcus mutans. Among the carbohydrate esters synthesized, galactose and fructose laurates showed the highest growth-inhibitory effect, whil...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010121
更新日期：2000-09-01 00:00:00
abstract：:A plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 (B-30488) was isolated from cows' milk. Bacterial colonization and growth responses of different plant species after inoculation with B-30488 were evaluated in a controlled environment and in microplot assays. Survival and colonization of B-30488 in the p...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0261-9
更新日期：2006-12-01 00:00:00
abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-008-9288-4
更新日期：2009-02-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010180
更新日期：2001-01-01 00:00:00
abstract：:Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial Sau3A digestion were cloned into the unique BamHI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC)promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900171
更新日期：1997-04-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900372
更新日期：1998-10-01 00:00:00
abstract：:Fourteen forms of endopolygalacturonases were purified from the culture medium of Sclerotinia sclerotiorum with ion exchange chromatographies. Individual forms differ in their isoelectric point but exhibit similar molecular masses. On the basis of the cross-reactions to antibodies raised against a purified acidic endo...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849900107
更新日期：1996-10-01 00:00:00
abstract：:Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with ...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-019-01737-1
更新日期：2019-10-01 00:00:00
abstract：:The antilisterial efficiency of three bacteriocins from lactic acid bacteria, lactocin 705 (produced by L. casei CRL705, 17000 AU/ml), enterocin CRL35 (produced by E. faecium CRL35, 17000 AU/ml), and nisin (2000 IU/ml), was tested in broth, individually and in combination against Listeria monocytogenes and Listeria in...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002840010159
更新日期：2000-12-01 00:00:00
abstract：:Lamprey was considered to be one of the most basal jawless vertebrate representatives for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. Here we investigated the effect of the gut-derived Aeromonas on the lamprey leukocytes proteome using the label-free liquid chromatography-ta...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-018-1468-2
更新日期：2018-07-01 00:00:00
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journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0440-8
更新日期：2007-06-01 00:00:00
abstract：:Cronobacter sakazakii is now recognized as an opportunistic pathogen and has been implicated in rare but severe cases of necrotizing enterocolitis, meningitis, and sepsis in neonates. The first step in bacterial pathogenesis requires that the organism adheres to host cells surfaces; therefore, agents that inhibit adhe...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-014-0623-7
更新日期：2014-10-01 00:00:00
abstract：:The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-006-0352-7
更新日期：2007-01-01 00:00:00
abstract：:A modification is described of the plate method for the detection of microorganisms with overall cellulolytic activity, including those like Cytophaga, in which the activity is cell bound. Within a few days of incubation colonies of cellulose-degrading bacteria formed holes in discs of lens paper placed on freshly ino...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s002849910027
更新日期：2000-02-01 00:00:00
abstract：:There is a region exhibiting a similarity of amino acid sequence near the carboxyl-terminal segment of each FAD-containing oxidoreductase. In this region, four amino acid residues-Thr, Ala, Gly, and Asp-are highly conserved. To determine the involvement of the four amino acid residues (Thr-469, Ala-476, Gly-478, and A...
journal_title：Current microbiology
pub_type： 杂志文章
doi：10.1007/s00284-002-3911-6
更新日期：2003-06-01 00:00:00
