Cgr1p, a novel nucleolar protein encoded by Saccharomyces cerevisiae orf YGL0292w.

Abstract:

:Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgr1p depletion on cell growth. Tagging Cgr1p with the green fluorescent protein (GFP) or the myc epitope showed focal accumulation of the fusion protein in the yeast nucleolus, and this localization overlapped with the distribution of the nucleolar protein Nop1p. Cells depleted of CGR1 mRNA were growth impaired and hypersensitive to the translational inhibitor paromomycin, and this phenotype was complemented by episomal expression of the CGR1-GFP fusion gene. These results identify Cgr1p as a novel component of the yeast nucleolus and suggest a potential role in ribosome biogenesis.

journal_name

Curr Microbiol

journal_title

Current microbiology

authors

Sun J,McFarland M,Boettner D,Panepinto J,Rhodes JC,Askew DS

doi

10.1007/s002840010180

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

65-9

issue

1

eissn

0343-8651

issn

1432-0991

pii

10.1007/s002840010180

journal_volume

42

pub_type

杂志文章
  • Proteomic analysis of the effect of cyanide on Klebsiella oxytoca.

    abstract::Cyanide has been proved to be degraded by Klebsiella oxytoca. In order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-DE) electrophoresis approach and MALDI-TOF-MS allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mM cyanid...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-009-9529-1

    authors: Tang P,Hseu YC,Chou HH,Huang KY,Chen SC

    更新日期:2010-03-01 00:00:00

  • Characterization of Comamonas thiooxidans sp. nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti.

    abstract::Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9602-9

    authors: Narayan KD,Pandey SK,Das SK

    更新日期:2010-10-01 00:00:00

  • The ability of 2-deoxyglucose to promote the lysis of Streptococcus bovis JB1 via a mechanism involving cell wall stability.

    abstract::The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cu...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900258

    authors: Russell JB,Wells JE

    更新日期:1997-11-01 00:00:00

  • Deletion of the CgTPI gene encoding triose phosphate isomerase of Candida glycerinogenes inhibits the biosynthesis of glycerol.

    abstract::The yeast Candida glycerinogenes produces a high yield of glycerol only in response to a medium-osmotic stress, but little is known about the relationship between osmoadaptation and glycerol metabolism. The CgTPI gene encoding triose phosphate isomerase of C. glycerinogenes was cloned and sequenced, and its functional...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-007-0070-9

    authors: Yongguang Z,Wei S,Zhiming R,Huiying F,Jian Z

    更新日期:2007-08-01 00:00:00

  • Identification of Cross Reactive Antigens of C. botulinum Types A, B, E & F by Immunoproteomic Approach.

    abstract::Diseases triggered by microorganisms can be controlled by vaccines, which need neutralizing antigens. Hence, it is very crucial to identify extremely efficient immunogens for immune prevention. Botulism, a fatal neuroparalytic disease, is caused by botulinum neurotoxins produced by the anaerobic, Gram-positive spore-f...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1413-9

    authors: Sharma A,Ponmariappan S,Sarita R,Alam SI,Kamboj DV,Shukla S

    更新日期:2018-05-01 00:00:00

  • Changes in Clostridium (Clostridioides) difficile PCR-Ribotype Distribution and Antimicrobial Resistance in a German Tertiary Care Hospital Over the Last 10 Years.

    abstract::Clostridium (Clostridioides) difficile ribotype 027 (RT027) was detected in Germany for the first time in 2007 during an outbreak in the region of Trier, Rhineland-Palatinate and is today the most prevalent ribotype (RT) in Europe. We aimed to determine the changes in RT distribution and corresponding antimicrobial re...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01654-3

    authors: Piepenbrock E,Stelzer Y,Berger F,Jazmati N

    更新日期:2019-04-01 00:00:00

  • Characterization of a novel Cr6+ reducing Pseudomonas sp. with plant growth-promoting potential.

    abstract::The isolate RNP4 obtained from a long-term tannery waste contaminated soil was characterized and presumptively identified as Pseudomonas sp. The strain RNP4 tolerated concentrations up to 450 mg Cr(6+)/L on a Luria-Bartani (LB) agar medium and reduced a substantial amount of Cr(6+) to Cr(3+) in the LB liquid medium. T...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-4470-4

    authors: Rajkumar M,Nagendran R,Lee KJ,Lee WH

    更新日期:2005-05-01 00:00:00

  • Influence of Phenotypic Dissociation in Bacillus subtilis Strain ET-1 on Iturin A Production.

    abstract::Iturin A is a very important cyclic lipopeptide produced by several B. subtilis strains and has large commercial and therapeutic application potentials but its production on industrial scale has not been realized yet. In the present study, we have observed that the strain ET-1 of Bacillus subtilis, a producer of Ituri...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01764-y

    authors: Ambrico A,Trupo M,Magarelli RA

    更新日期:2019-12-01 00:00:00

  • Antagonism Against Fish Pathogens by Cellular Components/Preparations of Bacillus coagulans (MTCC-9872) and It's In Vitro Probiotic Characterisation.

    abstract::Bacterial fish pathogens are pervasive in aquaculture. Control of bacterial fish pathogen is a difficult task among aquaculture practitioners. A large number of antibiotics are used for the control of prevalent bacterial pathogens in aquaculture. This may lead to drug resistance among pathogens and further treatment w...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-018-1506-0

    authors: Midhun SJ,Neethu S,Vysakh A,Radhakrishnan EK,Jyothis M

    更新日期:2018-09-01 00:00:00

  • Green fluorescent protein labeling Escherichia coli TG1 confirms intestinal bacterial translocation in a rat model of chemotherapy.

    abstract::It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-0177-9

    authors: Song D,Shi B,Xue H,Li Y,Yu B,Xu Z,Liu F,Li J

    更新日期:2006-01-01 00:00:00

  • Aniline-induced tryptophan production and identification of indole derivatives from three purple bacteria.

    abstract::Growth on aniline by three purple non-sulfur bacteria (Rhodospirillum rubrum ATCC 11170, Rhodobacter sphaeroides DSM 158, and Rubrivivax benzoatiliticus JA2) as nitrogen, or carbon source could not be demonstrated. However in its presence, production of indole derivatives was observed with all the strains tested. At l...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9609-2

    authors: Mujahid M,Sasikala Ch,Ramana ChV

    更新日期:2010-10-01 00:00:00

  • In-Vitro Prebiotic Analysis of Microbiota Accessible Pectic Polysaccharides.

    abstract::Pectin is a diverse polysaccharide comprising of polygalacturonic acid, rhamnogalactouronan, and neutral polysaccharides (arabinan and arabinogalactan) as side chains or branches. They are resistant to salivary amylase and gastric juice. In the present study the prebiotic potentials of different pectic derived substra...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-019-01781-x

    authors: Tingirikari JMR

    更新日期:2019-12-01 00:00:00

  • The community dynamics of major bioleaching microorganisms during chalcopyrite leaching under the effect of organics.

    abstract::To determine the effect of organics (yeast extract) on microbial community during chalcopyrite bioleaching at different temperature, real-time polymerase chain reaction (PCR) was employed to analyze community dynamics of major bacteria applied in bioleaching. The results showed that yeast extract exerted great impact ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-9960-y

    authors: Li Q,Tian Y,Fu X,Yin H,Zhou Z,Liang Y,Qiu G,Liu J,Liu H,Liang Y,Shen L,Cong J,Liu X

    更新日期:2011-08-01 00:00:00

  • Analysis of cell wall constituents of biocide-resistant isolates from dental-unit water line biofilms.

    abstract::In past years, the significance of microbial resistance to biocides has increased. Twenty biocide-resistant bacterial strains were isolated from dental-unit water line biofilm. All strains resisted high biocide concentrations (up to 100 microg ml(-1)): sodium dodecyl sulphate, hydrogen peroxide, sodium hypochlorite, p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-008-9200-2

    authors: Liaqat I,Sabri AN

    更新日期:2008-10-01 00:00:00

  • Enhanced tolerance against salt-stress and freezing-stress of Escherichia coli cells expressing algal bbc1 gene.

    abstract::The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in th...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002840010199

    authors: Tanaka S,Ikeda K,Miyasaka H

    更新日期:2001-03-01 00:00:00

  • Characterization of exopolysaccharides produced by cyanobacteria isolated from Polynesian microbial mats.

    abstract::Six cyanobacterial isolates recovered from Polynesian microbial mats, called "kopara," were cultured using laboratory-closed photobioreactors and were shown to produce exopolymers as released and capsular exopolysaccharides (EPS). These polymers have been chemically characterized using colorimetric and elemental assay...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-0069-z

    authors: Richert L,Golubic S,Guédès RL,Ratiskol J,Payri C,Guezennec J

    更新日期:2005-12-01 00:00:00

  • Cloning and expression of an alpha-amylase gene from Phanerochaete chrysosporium.

    abstract::Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an alpha-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known alpha-amylases ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0600-x

    authors: Wu B,Hu GK,Feng H,Wu JM,Zhang YZ

    更新日期:2007-08-01 00:00:00

  • Characterization and transcription of the genes encoding enzymes involved in butyrate production in Butyrivibrio fibrisolvens.

    abstract::Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not pr...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-002-3976-2

    authors: Asanuma N,Kawato M,Ohkawara S,Hino T

    更新日期:2003-09-01 00:00:00

  • Identification and partial sequence of the BchA gene of Rhodospirillum rubrum.

    abstract::The DNA sequence was determined for a region upstream of the puf operon of Rhodospirillum rubrum. A partial ORF was identified. The DNA sequence and the inferred amino acid sequences were aligned with those of bchA of Rhodobacter capsulatus and other phototrophic bacteria. Based on this alignment and genetic evidence,...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01570863

    authors: Lee IY,Collins ML

    更新日期:1993-08-01 00:00:00

  • PCR methods for identification and specific detection of probiotic lactic acid bacteria.

    abstract::Probiotics are defined as "microbes improving animal feed." Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide difference...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900082

    authors: Castellanos MI,Chauvet A,Deschamps A,Barreau C

    更新日期:1996-08-01 00:00:00

  • Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth.

    abstract::A total of 216 bacterial strains were isolated from rice rhizospheric soils in Northern Thailand. The bacterial strains were initially tested for solubilization of inorganic phosphate, indole acetic acid (IAA) production, selected strains were then tested for optimized conditions for IAA production and whether these c...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9674-6

    authors: Chaiharn M,Lumyong S

    更新日期:2011-01-01 00:00:00

  • Cloning of a laccase gene from a novel basidiomycete Trametes sp. 420 and its heterologous expression in Pichia pastoris.

    abstract::The laccase gene lacD, cloned from a novel laccase-producing basidiomycete Trametes sp. 420, contained 2,052 base pairs (bp) interrupted by 8 introns. lacD displayed a relatively high homology with laccase genes from other white rot fungi, whereas the homology between lacD and laccase genes from plants, insects, or ba...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0068-8

    authors: Hong YZ,Zhou HM,Tu XM,Li JF,Xiao YZ

    更新日期:2007-04-01 00:00:00

  • Isolation and characterization of transcription signal sequences from Streptococcus thermophilus.

    abstract::Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial Sau3A digestion were cloned into the unique BamHI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC)promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s002849900171

    authors: Solaiman DK,Somkuti GA

    更新日期:1997-04-01 00:00:00

  • Co-expression and synergism analysis of Vip3Aa29 and Cyt2Aa3 insecticidal proteins from Bacillus thuringiensis.

    abstract::Vegetative insecticidal protein (Vip3) from Bacillus thuringiensis shows high activity against lepidopteran insects. Cytolytic δ-endotoxin (Cyt) also has high toxicity to dipteran larvae and synergism with other crystal proteins (Cry), but synergism between Cyt and Vip3 proteins has not been tested. We analyzed for sy...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-011-0070-7

    authors: Yu X,Liu T,Sun Z,Guan P,Zhu J,Wang S,Li S,Deng Q,Wang L,Zheng A,Li P

    更新日期:2012-04-01 00:00:00

  • Degradation of mono-chlorinated dibenzo-p-dioxins by Janibacter sp. strain YA isolated from river sediment.

    abstract::Strain YA was newly isolated from an enrichment culture of river sediment and was identified as Janibacter sp. It was able to utilize dibenzofuran as the sole source of carbon and energy. Strain YA degraded > 90% of 1-chloro-dibenzo-p-dioxin (1-CDD) and > 80% of 2-chloro-dibenzo-p-dioxin in 18 hours with each initial ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-005-0099-6

    authors: Iwai S,Yamazoe A,Takahashi R,Kurisu F,Yagi O

    更新日期:2005-11-01 00:00:00

  • Shiga toxin-producing Escherichia coli O100:H⁻: stx2e in drinking water contaminated by waste water in Finland.

    abstract::In November 2007, 450 m(3) of treated wastewater leaked into the drinking water distribution system contaminating the drinking water of over 10,000 inhabitants of Nokia, Southern Finland. Nearly 1,000 people visited the health centre because of gastroenteritis during the following 5 weeks. A wide range of enteric path...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-010-9832-x

    authors: Lienemann T,Pitkänen T,Antikainen J,Mölsä E,Miettinen I,Haukka K,Vaara M,Siitonen A

    更新日期:2011-04-01 00:00:00

  • iTRAQ-MS/MS Proteomic Analysis Reveals Differentially Expressed Proteins During Post-harvest Maturation of the White Button Mushroom Agaricus bisporus.

    abstract::Agaricus bisporus (J. E. Lange) Imbach, well known as the common cultivated mushroom or white button mushroom, is widely cultivated all over the world. We used iTRAQ-MS/MS (i.e., isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry) to identify p...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-017-1225-y

    authors: Chen M,Liao J,Li H,Cai Z,Guo Z,Wach MP,Wang Z

    更新日期:2017-05-01 00:00:00

  • Effects of cations on antimicrobial activity of ostricacins-1 and 2 on E. coli O157:H7 and S. aureus 1056MRSA.

    abstract::Ostricacin-1 and ostricacin-2 (Osp-1 and Osp-2) were beta-defensins antimicrobial peptides that were purified from ostrich leukocytes using a cation-exchange column and a semi-prep RP-HPLC column. Both ostricacins were subjected to increased concentrations of monovalent cations (K(+) and Na(+)) and divalent cations (C...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-006-0554-z

    authors: Sugiarto H,Yu PL

    更新日期:2007-07-01 00:00:00

  • Roles of RPS41 in Biofilm Formation, Virulence, and Hydrogen Peroxide Sensitivity in Candida albicans.

    abstract::In eukaryotes, loss of cytoplasmic ribosomal proteins (RPs) results in a reduced growth rate and other phenotypic defects. The ability to transition from a unicellular budding yeast to a filamentous form is very important for biofilm formation and virulence in Candida albicans. Our recent study found that loss of the ...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-016-1019-7

    authors: Lu H,Xiong J,Shang Q,Jiang Y,Cao Y

    更新日期:2016-06-01 00:00:00

  • Fatty Acids Composition and Biofilm Production of Attenuated Salmonella typhimurium dam and seqA Mutants After Exposure to UV-C.

    abstract::The goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqA mutants in Salmonella typhimurium bacterial cells and UV-C radiations. The exposure changed the fatty acids composition of dam and seqA/dam strai...

    journal_title:Current microbiology

    pub_type: 杂志文章

    doi:10.1007/s00284-015-0858-y

    authors: Abdelwaheb C,Lobna M,Bouchra BA,Selma K,Ahmed L

    更新日期:2015-10-01 00:00:00