Abstract:
:The DNA sequence was determined for a region upstream of the puf operon of Rhodospirillum rubrum. A partial ORF was identified. The DNA sequence and the inferred amino acid sequences were aligned with those of bchA of Rhodobacter capsulatus and other phototrophic bacteria. Based on this alignment and genetic evidence, this ORF was identified as R. rubrum bchA, which encodes an enzyme involved in bacteriochlorophyll biosynthesis. An additional ORF was identified in the intergenic region between bchA and pufB. Sequences possibly having a role in puf expression were identified.
journal_name
Curr Microbioljournal_title
Current microbiologyauthors
Lee IY,Collins MLdoi
10.1007/BF01570863subject
Has Abstractpub_date
1993-08-01 00:00:00pages
85-90issue
2eissn
0343-8651issn
1432-0991journal_volume
27pub_type
杂志文章abstract::Strain YA was newly isolated from an enrichment culture of river sediment and was identified as Janibacter sp. It was able to utilize dibenzofuran as the sole source of carbon and energy. Strain YA degraded > 90% of 1-chloro-dibenzo-p-dioxin (1-CDD) and > 80% of 2-chloro-dibenzo-p-dioxin in 18 hours with each initial ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0099-6
更新日期:2005-11-01 00:00:00
abstract::To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9426-7
更新日期:2009-09-01 00:00:00
abstract::The esterase-encoding gene, estA, was cloned from Acinetobacter lwoffii I6C-1 genomic DNA into Escherichia coli BL21(DE3) with plasmid vector pET-22b (pEM1). pEM1 has a 4.4-kb EcoRI insert that contained the complete estA gene. A 2.4-kb AvaI- SphI DNA fragment was subcloned (pEM3) and sequenced. estA gene encodes a pr...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3886-3
更新日期:2003-04-01 00:00:00
abstract::Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM an...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900319
更新日期:1998-06-01 00:00:00
abstract::Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further e...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0218-4
更新日期:2006-02-01 00:00:00
abstract::The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/BF01577379
更新日期:1993-04-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9256-z
更新日期:2009-01-01 00:00:00
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journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-02028-w
更新日期:2020-09-01 00:00:00
abstract::Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D, and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were r...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s002849900438
更新日期:1999-09-01 00:00:00
abstract::Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9572-y
更新日期:2010-07-01 00:00:00
abstract::The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unc...
journal_title:Current microbiology
pub_type: 信件
doi:10.1007/s00284-017-1207-0
更新日期:2017-03-01 00:00:00
abstract::The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. or...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1350-7
更新日期:2018-01-01 00:00:00
abstract::Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment len...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0355-9
更新日期:2006-05-01 00:00:00
abstract::Mycoplasma penetrans invades HeLa cells and survives within them for prolonged periods of time. The intracellular distribution of M. penetrans within HeLa cells was studied utilizing the acidotropic dye LysoTracker (green), which permeates cell membranes and upon protonation remains trapped in acidic compartments. The...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-004-4298-3
更新日期:2004-08-01 00:00:00
abstract::This study genetically screened endophytic and epiphytic bacteria associated with ethnomedicinal plants for the presence of the non-ribosomal peptide synthetase (NRPS) gene and identified horizontal gene transfer (HGT) of the NRPS gene between the bacterial species. NRPSs are large multimodular enzymes that synthesize...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-015-0910-y
更新日期:2016-01-01 00:00:00
abstract::Yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental pH. Previous results have indicated that secretion of the alkaline protease is under control of the pH signaling Pal/Rim pathway originally described in Aspergillus nidulans. Several Y. lipol...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-005-0070-6
更新日期:2006-07-01 00:00:00
abstract::Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for inciden...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9243-4
更新日期:2008-12-01 00:00:00
abstract::Cutaneous fungal infections are common and widespread. Antifungal agents used for the treatment of these infections often have undesirable side effects. Furthermore, increased resistance of the microorganisms to the antifungal drugs becomes the growing problem. Accordingly, the search for natural antifungal compounds ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-014-0585-9
更新日期:2014-09-01 00:00:00
abstract::A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9469-9
更新日期:2009-11-01 00:00:00
abstract::This work compared the inhibition effect of the commercially available mouthwash Corsodyl, containing 0.1% chlorhexidine digluconate, and photodynamic inactivation (PDI) employing methylene blue (MB) with irradiation from a red laser on 24-h biofilms formed by Streptococcus mutans strains on hydroxyapatite surfaces. T...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-020-01901-y
更新日期:2020-06-01 00:00:00
abstract::The biogenic amine-producing capability of several Oenococcus oeni strains, originally isolated from different Italian wines, was determined. The amine-producing capability was quali-quantitatively variable among the strains: out of the 44 strains investigated under optimal growth conditions, more than 60% were able t...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-001-0021-9
更新日期:2002-05-01 00:00:00
abstract::The authors would like to correct the errors in the publication of the original article. ...
journal_title:Current microbiology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00284-020-01947-y
更新日期:2020-08-01 00:00:00
abstract::In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study m...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-013-0317-6
更新日期:2013-06-01 00:00:00
abstract::Serratia marcescens is an opportunistic pathogen responsible for causing nosocomial infections, corneal ulcer, necrotizing fasciitis, cellulites, and brain abscess. Alkaline phosphatase (APase) is believed to play an important role in the survival of several intracellular pathogens and their adaptation. We have studie...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-002-3740-7
更新日期:2002-10-01 00:00:00
abstract::Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-009-9386-y
更新日期:2009-06-01 00:00:00
abstract::In this study, fatty-acid content and patterns were analyzed in order to distinguish Nostoc flagelliforme, an edible terrestrial cyanobacterium, from other Nostoc species and representatives typical of its close neighbors (genera Anabaena, Microcystis, and Synechococcus). According to the Kenyon-Murata classification ...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-003-4059-8
更新日期:2003-12-01 00:00:00
abstract::The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extract...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-011-9977-2
更新日期:2011-09-01 00:00:00
abstract::The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P29...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-017-1402-z
更新日期:2018-04-01 00:00:00
abstract::The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-008-9351-1
更新日期:2009-05-01 00:00:00
abstract::Comamonas thiooxidans (strain S23(T)) capable of oxidizing thiosulfate under a mixotrophic growth condition was isolated from a sulfur spring. DNA-DNA homology study showed 55% similarity with Comamonas testosteroni KCTC2990(T) and 52% with Comamonas composti LMG24008(T), the nearest phylogenetic relative (16S rRNA se...
journal_title:Current microbiology
pub_type: 杂志文章
doi:10.1007/s00284-010-9602-9
更新日期:2010-10-01 00:00:00